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Osanloo, Elham, Soleymani, Ali Asghar, Taheri, Marziyeh, Hosseini, Sareh Sadat, Javadi, Fatemeh, Karimi-Yazdi, Mohammadmahdi, Agha Gholizadeh, Majid, Hakemi-Vala, Mojdeh. (1402). Evaluation of Antibacterial, Antibiofilm and Cytotoxicity Effect of Crude Ethanolic Extract of Cuminum cyminum Against Streptococcus mutans. سامانه مدیریت نشریات علمی, 12(2), 191-196. doi: 10.22092/jmpb.2021.356243.1412
Elham Osanloo; Ali Asghar Soleymani; Marziyeh Taheri; Sareh Sadat Hosseini; Fatemeh Javadi; Mohammadmahdi Karimi-Yazdi; Majid Agha Gholizadeh; Mojdeh Hakemi-Vala. "Evaluation of Antibacterial, Antibiofilm and Cytotoxicity Effect of Crude Ethanolic Extract of Cuminum cyminum Against Streptococcus mutans". سامانه مدیریت نشریات علمی, 12, 2, 1402, 191-196. doi: 10.22092/jmpb.2021.356243.1412
Osanloo, Elham, Soleymani, Ali Asghar, Taheri, Marziyeh, Hosseini, Sareh Sadat, Javadi, Fatemeh, Karimi-Yazdi, Mohammadmahdi, Agha Gholizadeh, Majid, Hakemi-Vala, Mojdeh. (1402). 'Evaluation of Antibacterial, Antibiofilm and Cytotoxicity Effect of Crude Ethanolic Extract of Cuminum cyminum Against Streptococcus mutans', سامانه مدیریت نشریات علمی, 12(2), pp. 191-196. doi: 10.22092/jmpb.2021.356243.1412
Osanloo, Elham, Soleymani, Ali Asghar, Taheri, Marziyeh, Hosseini, Sareh Sadat, Javadi, Fatemeh, Karimi-Yazdi, Mohammadmahdi, Agha Gholizadeh, Majid, Hakemi-Vala, Mojdeh. Evaluation of Antibacterial, Antibiofilm and Cytotoxicity Effect of Crude Ethanolic Extract of Cuminum cyminum Against Streptococcus mutans. سامانه مدیریت نشریات علمی, 1402; 12(2): 191-196. doi: 10.22092/jmpb.2021.356243.1412

Evaluation of Antibacterial, Antibiofilm and Cytotoxicity Effect of Crude Ethanolic Extract of Cuminum cyminum Against Streptococcus mutans

Journal of Medicinal plants and By-product
دوره 12، شماره 2، شهریور 2023، صفحه 191-196    اصل مقاله (611.59 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2021.356243.1412
نویسندگان
Elham Osanloo1؛ Ali Asghar Soleymani1؛ Marziyeh Taheri2؛ Sareh Sadat Hosseini2؛ Fatemeh Javadi1؛ Mohammadmahdi Karimi-Yazdi3؛ Majid Agha Gholizadeh1؛ Mojdeh Hakemi-Vala* 2
1Department of Pediatric Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences (SBMU) Tehran, Iran
2Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences (SBMU) Tehran, Iran
3Faculty of Paramedical Sciences, Mazandaran University of Medical Sciences, Sari, Iran
چکیده
Although, oral health is important for its role in the overall health but oral health care does not have an integrated model. So, today meeting the needs of oral health requires strong interactions between health research policy and oral health researchThe aim of this study investigates the effects of ethanolic crud extract of Cuminum cyminum L. on S. mutans in terms of antibacterial, antibiofilm and, its cytotoxicity properties. The present experimental study which has conducted in 2019-2020. The crude alcoholic extract of C. cyminum seeds was prepared by the maceration method. Then, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of crude ethanolic extract of C. cyminum was determined by micro-dilution method according to CLSI protocol on Streptococcus mutans (PTCC1683). In addition, its antibiofilm effect was investigated by the previous method using crystal violet. As well, an MTT test was performed to evaluate its cytotoxicity on SW480 cells. Statistical analysis was done by SPSS V.22 software. MIC and MBC concentrations of C. cyminum extract on S. mutans were found to be 0.62 mg/mL and 1.25 mg/mL. The effect of inhibiting biofilm production was also observed at a concentration of 40 mg/mL.  In MTT assay showed a greater cytotoxic effect on SW480 cancer cells at higher concentrations for longer periods of time. The present study showed that the cumin ethanolic crude extract has antimicrobial properties, inhibits the growth of S. mutans biofilms and has no toxic properties.
MIC: minimum inhibitory concentration. MBC: minimum bactericidal concentration. MTT: 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyl-2H- tetrazolium bromide. DMSO: dimethyl sulfoxide
کلیدواژه‌ها
Cumin؛ Dental carriers؛ Biofilm؛ S. mutans
مراجع
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