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Mohammadi Mollaahmadi, C, Anzabi, Y, Shayegh, J. (1400). Comparison of the Frequency of Biofilm-Forming Genes (icaABCD) in Methicillin-Resistant S. aureus Strains Isolated from Human and Livestock. سامانه مدیریت نشریات علمی, 76(6), 1655-1663. doi: 10.22092/ari.2020.351381.1522
C Mohammadi Mollaahmadi; Y Anzabi; J Shayegh. "Comparison of the Frequency of Biofilm-Forming Genes (icaABCD) in Methicillin-Resistant S. aureus Strains Isolated from Human and Livestock". سامانه مدیریت نشریات علمی, 76, 6, 1400, 1655-1663. doi: 10.22092/ari.2020.351381.1522
Mohammadi Mollaahmadi, C, Anzabi, Y, Shayegh, J. (1400). 'Comparison of the Frequency of Biofilm-Forming Genes (icaABCD) in Methicillin-Resistant S. aureus Strains Isolated from Human and Livestock', سامانه مدیریت نشریات علمی, 76(6), pp. 1655-1663. doi: 10.22092/ari.2020.351381.1522
Mohammadi Mollaahmadi, C, Anzabi, Y, Shayegh, J. Comparison of the Frequency of Biofilm-Forming Genes (icaABCD) in Methicillin-Resistant S. aureus Strains Isolated from Human and Livestock. سامانه مدیریت نشریات علمی, 1400; 76(6): 1655-1663. doi: 10.22092/ari.2020.351381.1522

Comparison of the Frequency of Biofilm-Forming Genes (icaABCD) in Methicillin-Resistant S. aureus Strains Isolated from Human and Livestock

Archives of Razi Institute
مقاله 10، دوره 76، شماره 6، بهمن و اسفند 2021، صفحه 1655-1663  XML اصل مقاله (535.55 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2020.351381.1522
نویسندگان
C Mohammadi Mollaahmadi1؛ Y Anzabi email orcid 2، 3؛ J Shayegh4
1Faculty of Basic Sciences, Department of Microbiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3Biotechnology Research Center, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4Department of Veterinary Medicine, Islamic Azad University, Shabestar Branch, Islamic Azad University, Shabestar, Iran
چکیده
Methicillin-resistant Staphylococcus aureus (MRSA) can cause infections in both human and animal groups, which is a serious threat to public health worldwide. Attachment and colonization are the first steps for S. aureus pathogenesis, and biofilm-mediated infections have a significant negative impact on human and animal health. The MRSA can adapt to different environments and give rise to different strains of human and animal MRSA, causing transmissions of the disease between humans and animals. This study aimed to investigate biofilm production in vitro, and the presence of icaABCD genes in MRSA isolates in both human as well as the disease transmission between human and animal strains. In total, 39 human and 35 livestock isolates were evaluated by the Congo Red Agar method. The presence of mecA and icaABCDR genes were assessed by polymerase chain reaction (PCR), and finally, the PCR products were examined by agarose gel electrophoresis. The results showed that the mecA gene frequency in human and animal isolates was 64.1% and 36.1%, respectively, and there was a significant relationship between mecA and icaAD in human isolates. In addition, significant relationships were found between icaA and Rifampicin and also between icaC and Chloramphenicol and Penicillin in human isolates. In animal isolates, there was a significant relationship between mecA and Trimethoprim as well as between icaR and Rifampicin. It was concluded that all operon ica genes were involved in biofilm production, but icaA and icaD genes in MRSA were more closely associated with mecA. Both animal and human strains can be involved in disease transmission, but this conclusion should be made cautiously.
کلیدواژه‌ها
Bovine mastitis؛ Clinical؛ icaABCD؛ MRSA؛ Staphylococcus aureus
عنوان مقاله [English]
مقایسه فراوانی ژنهای سازنده بیوفیلم (icaABCD) در سویه های مقاوم به متی سیلین استافیلوکوکوس اورئوس جدا شده از انسان و دام
چکیده [English]
MRSA که باعث ایجاد عفونت در هر دو گروه انسانی و حیوانی می شود، تهدیدی جدی برای بهداشت عمومی در سراسر جهان است. اتصال و کلونیزاسیون اولین مرحله برای پاتوژنز استافیلوکوکوس اورئوس می باشند و عفونت های بیوفیلم تأثیر منفی قابل توجهی بر سلامت انسان و دام دارند. استافیلوکوکوس اورئوس مقاوم به متی سیلین می تواند با محیط های مختلف سازگار گشته، باعث بوجود آمدن سویه های مختلف استافیلوکوکوس اورئوس مقاوم به متی سیلین در انسان و حیوان شده و بینشان انتقال یابد. این مطالعه با هدف بررسی تولید بیوفیلم در شرایط in vitro و وجود ژن های icaABCD در جدایه های S. aureus مقاوم به متی سیلین (MRSA) در انسان و بررسی انتقال بیماری بین سویه های انسانی و حیوانی انجام می شود. در مجموع، 39 جدایه انسانی و 35 جدایه دامی با استفاده از روش کنگورد آگار مورد ارزیابی قرار گرفتند. حضور ژن های mecA و icaABCDR توسط PCR بررسی شده و در نهایت، محصولات PCR توسط الکتروفورز با ژل آگارز مورد بررسی قرار گرفتند. نتایج حاصله نشانگر این است که فراوانی ژن mecA در جدایه های انسان و حیوان به ترتیب 64.1 و 36.1 درصد بوده و در جدایه های انسانی بین mecA و icaAD رابطه معنی داری وجود داشت. علاوه بر این، ارتباط معناداری بین icaA و ریفامپیسین و همچنین بین icaC و کلرامفنیکل و پنی سیلین در جدایه های انسانی یافت شد. در جدایه های دامی نیز، رابطه معنی داری بین mecA و تریمتوپریم و همچنین بین icaR و ریفامپیسین وجود داشت. نتیجه گیری شد که همه ژن های اپرون ica در تولید بیوفیلم نقش دارند، ولی ژن های icaA و icaD در استافیلوکوکوس اورئوس مقاوم به متی سیلین با mecA همراهی بیشتری داشتند. همچنین سویه های حیوانی و انسانی می توانند در انتقال بیماری نقش داشته باشند، اما این نتیجه گیری باید با احتیاط بیشتری بیان شود.
کلیدواژه‌ها [English]
استافیلوکوکوس اورئوس, استافیلوکوکوس اورئوس های مقاوم به متی سیلین, بالینی, ورم پستان گاوی, icaABCD
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