اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,041
تعداد مقالات34,003
تعداد مشاهده مقاله46,119,444
تعداد دریافت فایل اصل مقاله77,314,074
Mohammadi Mollaahmadi, C, Anzabi, Y, Shayegh, J. (1400). Comparison of the Frequency of Biofilm-Forming Genes (icaABCD) in Methicillin-Resistant S. aureus Strains Isolated from Human and Livestock. سامانه مدیریت نشریات علمی, 76(6), 1655-1663. doi: 10.22092/ari.2020.351381.1522
C Mohammadi Mollaahmadi; Y Anzabi; J Shayegh. "Comparison of the Frequency of Biofilm-Forming Genes (icaABCD) in Methicillin-Resistant S. aureus Strains Isolated from Human and Livestock". سامانه مدیریت نشریات علمی, 76, 6, 1400, 1655-1663. doi: 10.22092/ari.2020.351381.1522
Mohammadi Mollaahmadi, C, Anzabi, Y, Shayegh, J. (1400). 'Comparison of the Frequency of Biofilm-Forming Genes (icaABCD) in Methicillin-Resistant S. aureus Strains Isolated from Human and Livestock', سامانه مدیریت نشریات علمی, 76(6), pp. 1655-1663. doi: 10.22092/ari.2020.351381.1522
Mohammadi Mollaahmadi, C, Anzabi, Y, Shayegh, J. Comparison of the Frequency of Biofilm-Forming Genes (icaABCD) in Methicillin-Resistant S. aureus Strains Isolated from Human and Livestock. سامانه مدیریت نشریات علمی, 1400; 76(6): 1655-1663. doi: 10.22092/ari.2020.351381.1522

Comparison of the Frequency of Biofilm-Forming Genes (icaABCD) in Methicillin-Resistant S. aureus Strains Isolated from Human and Livestock

Archives of Razi Institute
مقاله 10، دوره 76، شماره 6، بهمن و اسفند 2021، صفحه 1655-1663    اصل مقاله (535.88 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2020.351381.1522
نویسندگان
C Mohammadi Mollaahmadi1؛ Y Anzabi* 2، 3؛ J Shayegh4
1Faculty of Basic Sciences, Department of Microbiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3Biotechnology Research Center, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4Department of Veterinary Medicine, Islamic Azad University, Shabestar Branch, Islamic Azad University, Shabestar, Iran
چکیده
Methicillin-resistant Staphylococcus aureus (MRSA) can cause infections in both human and animal groups, which is a serious threat to public health worldwide. Attachment and colonization are the first steps for S. aureus pathogenesis, and biofilm-mediated infections have a significant negative impact on human and animal health. The MRSA can adapt to different environments and give rise to different strains of human and animal MRSA, causing transmissions of the disease between humans and animals. This study aimed to investigate biofilm production in vitro, and the presence of icaABCD genes in MRSA isolates in both human as well as the disease transmission between human and animal strains. In total, 39 human and 35 livestock isolates were evaluated by the Congo Red Agar method. The presence of mecA and icaABCDR genes were assessed by polymerase chain reaction (PCR), and finally, the PCR products were examined by agarose gel electrophoresis. The results showed that the mecA gene frequency in human and animal isolates was 64.1% and 36.1%, respectively, and there was a significant relationship between mecA and icaAD in human isolates. In addition, significant relationships were found between icaA and Rifampicin and also between icaC and Chloramphenicol and Penicillin in human isolates. In animal isolates, there was a significant relationship between mecA and Trimethoprim as well as between icaR and Rifampicin. It was concluded that all operon ica genes were involved in biofilm production, but icaA and icaD genes in MRSA were more closely associated with mecA. Both animal and human strains can be involved in disease transmission, but this conclusion should be made cautiously.
کلیدواژه‌ها
Bovine mastitis؛ Clinical؛ icaABCD؛ MRSA؛ Staphylococcus aureus
عنوان مقاله [English]
مقایسه فراوانی ژنهای سازنده بیوفیلم (icaABCD) در سویه های مقاوم به متی سیلین استافیلوکوکوس اورئوس جدا شده از انسان و دام
چکیده [English]
MRSA که باعث ایجاد عفونت در هر دو گروه انسانی و حیوانی می شود، تهدیدی جدی برای بهداشت عمومی در سراسر جهان است. اتصال و کلونیزاسیون اولین مرحله برای پاتوژنز استافیلوکوکوس اورئوس می باشند و عفونت های بیوفیلم تأثیر منفی قابل توجهی بر سلامت انسان و دام دارند. استافیلوکوکوس اورئوس مقاوم به متی سیلین می تواند با محیط های مختلف سازگار گشته، باعث بوجود آمدن سویه های مختلف استافیلوکوکوس اورئوس مقاوم به متی سیلین در انسان و حیوان شده و بینشان انتقال یابد. این مطالعه با هدف بررسی تولید بیوفیلم در شرایط in vitro و وجود ژن های icaABCD در جدایه های S. aureus مقاوم به متی سیلین (MRSA) در انسان و بررسی انتقال بیماری بین سویه های انسانی و حیوانی انجام می شود. در مجموع، 39 جدایه انسانی و 35 جدایه دامی با استفاده از روش کنگورد آگار مورد ارزیابی قرار گرفتند. حضور ژن های mecA و icaABCDR توسط PCR بررسی شده و در نهایت، محصولات PCR توسط الکتروفورز با ژل آگارز مورد بررسی قرار گرفتند. نتایج حاصله نشانگر این است که فراوانی ژن mecA در جدایه های انسان و حیوان به ترتیب 64.1 و 36.1 درصد بوده و در جدایه های انسانی بین mecA و icaAD رابطه معنی داری وجود داشت. علاوه بر این، ارتباط معناداری بین icaA و ریفامپیسین و همچنین بین icaC و کلرامفنیکل و پنی سیلین در جدایه های انسانی یافت شد. در جدایه های دامی نیز، رابطه معنی داری بین mecA و تریمتوپریم و همچنین بین icaR و ریفامپیسین وجود داشت. نتیجه گیری شد که همه ژن های اپرون ica در تولید بیوفیلم نقش دارند، ولی ژن های icaA و icaD در استافیلوکوکوس اورئوس مقاوم به متی سیلین با mecA همراهی بیشتری داشتند. همچنین سویه های حیوانی و انسانی می توانند در انتقال بیماری نقش داشته باشند، اما این نتیجه گیری باید با احتیاط بیشتری بیان شود.
کلیدواژه‌ها [English]
استافیلوکوکوس اورئوس, استافیلوکوکوس اورئوس های مقاوم به متی سیلین, بالینی, ورم پستان گاوی, icaABCD
سایر فایل های مرتبط با مقاله
مراجع
  1. DeLeo FR, Chambers HF. Reemergence of antibiotic-resistant Staphylococcus aureus in the genomics era. J Clin Invest. 2009;119(9):2464-74.
  2. Ateba C, Bezuidenhout C, Moneoang M, Mbewe M. Antibiotic resistant Staphylococcus aureus isolated from milk in the Mafikeng Area, North-West Province, South Africa. S Afr J Sci. 2010;106:1-6.
  3. Fischetti VA, Novick RP, Ferretti JJ, Portnoy DA, Braunstein M, Rood JI. Gram-positive pathogens. 3rd ed John Wiley & Sons. 2019.
  4. Turlej A, Hryniewicz W, Empel J. Staphylococcal cassette chromosome mec (Sccmec) classification and typing methods: an overview. Pol J Microbiol. 2011;60(2):95-103.
  5. Arciola CR, Campoccia D, Montanaro L. Detection of biofilm-forming strains of Staphylococcus epidermidis and S. aureus. Expert Rev Mol Diagn. 2002;2(5):478-84.
  6. Fitzpatrick F, Humphreys H, O'Gara JP. The genetics of staphylococcal biofilm formation--will a greater understanding of pathogenesis lead to better management of device-related infection? Clin Microbiol Infect. 2005;11(12):967-73.
  7. Deurenberg RH, Vink C, Kalenic S, Friedrich AW, Bruggeman CA, Stobberingh EE. The molecular evolution of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect. 2007;13(3):222-35.
  8. Pontes DS, Pinheiro FA, Lima-Bittencourt CI, Guedes RL, Cursino L, Barbosa F, et al. Multiple antimicrobial resistance of gram-negative bacteria from natural oligotrophic lakes under distinct anthropogenic influence in a tropical region. Microb Ecol. 2009;58(4):762-72.
  9. Sabath LD, Wheeler N, Laverdiere M, Blazevic D, Wilkinson BJ. A new type of penicillin resistance of Staphylococcus aureus. Lancet (London, England). 1977;1(8009):443-7.
  10. de Almeida CC, Pizauro LJL, Soltes GA, Slavic D, de Avila FA, Pizauro JM, et al. Some coagulase negative Staphylococcus spp. isolated from buffalo can be misidentified as Staphylococcus aureus by phenotypic and Sa442 PCR methods. BMC Res Notes. 2018;11(1):346.
  11. García-Álvarez L, Holden MTG, Lindsay H, Webb CR, Brown DFJ, Curran MD, et al. Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. Lancet Infect Dis. 2011;11(8):595-603.
  12. Rohde H, Knobloch JK, Horstkotte MA, Mack D. Correlation of Staphylococcus aureus icaADBC genotype and biofilm expression phenotype. J Clin Microbiol. 2001;39(12):4595-6.
  13. Salas M, Wernecki M, Fernandez L, Iglesias B, Gutierrez D, Alvarez A, et al. Characterization of Clinical MRSA Isolates from Northern Spain and Assessment of Their Susceptibility to Phage-Derived Antimicrobials. Antibiotics (Basel). 2020;9(8).
  14. Pirolo M, Visaggio D, Gioffre A, Artuso I, Gherardi M, Pavia G, et al. Unidirectional animal-to-human transmission of methicillin-resistant Staphylococcus aureus ST398 in pig farming; evidence from a surveillance study in southern Italy. Antimicrob Resist Infect Control. 2019;8:187.
  15. Bardiau M, Yamazaki K, Duprez JN, Taminiau B, Mainil JG, Ote I. Genotypic and phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) isolated from milk of bovine mastitis. Lett Appl Microbiol. 2013;57(3):181-6.
  16. Martin MJ, Thottathil SE, Newman TB. Antibiotics Overuse in Animal Agriculture: A Call to Action for Health Care Providers. Am J Public Health. 2015;105(12):2409-10.
  17. Smith TC, Male MJ, Harper AL, Kroeger JS, Tinkler GP, Moritz ED, et al. Methicillin-resistant Staphylococcus aureus (MRSA) strain ST398 is present in midwestern U.S. swine and swine workers. PLoS One. 2009;4(1):4258.
  18. Wassenberg MW, Kluytmans JA, Box AT, Bosboom RW, Buiting AG, van Elzakker EP, et al. Rapid screening of methicillin-resistant Staphylococcus aureus using PCR and chromogenic agar: a prospective study to evaluate costs and effects. Clin Microbiol Infect. 2010;16(12):1754-61.
  19. Smith TC. Livestock-associated Staphylococcus aureus: the United States experience. PLoS Pathog. 2015;11(2):1004564.
  20. Smith DL, Harris AD, Johnson JA, Silbergeld EK, Morris JG, Jr. Animal antibiotic use has an early but important impact on the emergence of antibiotic resistance in human commensal bacteria. Proc Natl Acad Sci U S A. 2002;99(9):6434-9.
  21. Wu S, Piscitelli C, de Lencastre H, Tomasz A. Tracking the evolutionary origin of the methicillin resistance gene: cloning and sequencing of a homologue of mecA from a methicillin susceptible strain of Staphylococcus sciuri. Microb Drug Resist. 1996;2(4):435-41.
  22. Shore AC, Deasy EC, Slickers P, Brennan G, O'Connell B, Monecke S, et al. Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2011;55(8):3765-73.
  23. Lowder BV, Guinane CM, Ben Zakour NL, Weinert LA, Conway-Morris A, Cartwright RA, et al. Recent human-to-poultry host jump, adaptation, and pandemic spread of Staphylococcus aureus. Proc Natl Acad Sci U S A. 2009;106(46):19545-50.
  24. Sakwinska O, Giddey M, Moreillon M, Morisset D, Waldvogel A, Moreillon P. Staphylococcus aureus host range and human-bovine host shift. Appl Environ Microbiol. 2011;77(17):5908-15.
  25. Weese JS, Caldwell F, Willey BM, Kreiswirth BN, McGeer A, Rousseau J, et al. An outbreak of methicillin-resistant Staphylococcus aureus skin infections resulting from horse to human transmission in a veterinary hospital. Vet Microbiol. 2006;114(1-2):160-4.
  26. Spoor LE, McAdam PR, Weinert LA, Rambaut A, Hasman H, Aarestrup FM, et al. Livestock origin for a human pandemic clone of community-associated methicillin-resistant Staphylococcus aureus. mBio. 2013;4(4).
  27. Haenni M, Saras E, Chatre P, Medaille C, Bes M, Madec JY, et al. A USA300 variant and other human-related methicillin-resistant Staphylococcus aureus strains infecting cats and dogs in France. J Antimicrob Chemother. 2012;67(2):326-9.
  28. Price LB, Stegger M, Hasman H, Aziz M, Larsen J, Andersen PS, et al. Staphylococcus aureus CC398: host adaptation and emergence of methicillin resistance in livestock. mBio. 2012;3(1).
  29. de Boer E, Zwartkruis-Nahuis JT, Wit B, Huijsdens XW, de Neeling AJ, Bosch T, et al. Prevalence of methicillin-resistant Staphylococcus aureus in meat. Int J Food Microbiol. 2009;134(1-2):52-6.
  30. Pu S, Han F, Ge B. Isolation and characterization of methicillin-resistant Staphylococcus aureus strains from Louisiana retail meats. Appl Environ Microbiol. 2009;75(1):265-7.
  31. Hammad AM, Watanabe W, Fujii T, Shimamoto T. Occurrence and characteristics of methicillin-resistant and -susceptible Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci from Japanese retail ready-to-eat raw fish. Int J Food Microbiol. 2012;156(3):286-9.
  32. Chon J, Sung K, Khan S. Methicillin-Resistant Staphylococcus aureus (MRSA) in Food- Producing and Companion Animals and Food Products.  Frontiers in Staphylococcus aureus: InTech; 2017. p. 47- 102.
  1. Costerton JW, Montanaro L, Arciola CR. Biofilm in implant infections: its production and regulation. Int J Artif Organs. 2005;28(11):1062-8.
  2. Arciola CR, Baldassarri L, Montanaro L. Presence of icaA and icaD genes and slime production in a collection of staphylococcal strains from catheter-associated infections. J Clin Microbiol. 2001;39(6):2151-6.
  3. Piechota M, Kot B, Frankowska-Maciejewska A, Gruzewska A, Wozniak-Kosek A. Biofilm Formation by Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains from Hospitalized Patients in Poland. Biomed Res Int. 2018;2018:4657396.
  4. Bimanand L, Taherikalani M, Jalilian FA, Sadeghifard N, Ghafourian S, Mahdavi Z, et al. Association between biofilm production, adhesion genes and drugs resistance in different SCCmec types of methicillin resistant Staphylococcus aureus strains isolated from several major hospitals of Iran. Iran J Basic Med Sci. 2018;21(4):400-3.
  5. Ghasemian A, Najar Peerayeh S, Bakhshi B, Mirzaee M. Comparison of Biofilm Formation between Methicillin-Resistant and Methicillin-Susceptible Isolates of Staphylococcus aureus. Iran Biomed J. 2016;20(3):175-81.
  6. Serray B, Oufrid S, Hannaoui I, Bourjilate F, Soraa N, Mliji M, et al. Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco. J Infect Dev Ctries. 2016;10(8):863-9.
  7. Nourbakhsh F, Momtaz H. Evaluation of Phenotypic and Genotypic Biofilm Formation in Staphylococcus aureus Isolates Isolated from Hospital Infections in Shahrekord, 2015. J Arak Univ Med Sci. 2016;19(4):69-79.
  8. Ohadian Moghadam S, Pourmand MR, Aminharati F. Biofilm formation and antimicrobial resistance in methicillin-resistant Staphylococcus aureus isolated from burn patients, Iran. J Infect Dev Ctries. 2014;8(12):1511-7.
  9. Mirzaee M, Najar-Peerayeh S, Behmanesh M, Forouzandeh Moghadam M, Ghasemian A-M. Biofilm Formation and Presence of ica Genes in Staphylococcus aureus Isolated from Intensive Care Unit. J Maz Univ Med Sci. 2014;24(115):43-51.
 

آمار
تعداد مشاهده مقاله: 14,791
تعداد دریافت فایل اصل مقاله: 56,156


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب