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Salih, R. A, Mohamed, N. S, Taha, A. A. (1401). Genetic Sequence of Coronavirus Strains Isolated from Iraqi Patients and their Relationship with some Liver Enzymes and Interleukins. سامانه مدیریت نشریات علمی, 77(2), 809-819. doi: 10.22092/ari.2022.357089.1967
R. A Salih; N. S Mohamed; A. A Taha. "Genetic Sequence of Coronavirus Strains Isolated from Iraqi Patients and their Relationship with some Liver Enzymes and Interleukins". سامانه مدیریت نشریات علمی, 77, 2, 1401, 809-819. doi: 10.22092/ari.2022.357089.1967
Salih, R. A, Mohamed, N. S, Taha, A. A. (1401). 'Genetic Sequence of Coronavirus Strains Isolated from Iraqi Patients and their Relationship with some Liver Enzymes and Interleukins', سامانه مدیریت نشریات علمی, 77(2), pp. 809-819. doi: 10.22092/ari.2022.357089.1967
Salih, R. A, Mohamed, N. S, Taha, A. A. Genetic Sequence of Coronavirus Strains Isolated from Iraqi Patients and their Relationship with some Liver Enzymes and Interleukins. سامانه مدیریت نشریات علمی, 1401; 77(2): 809-819. doi: 10.22092/ari.2022.357089.1967

Genetic Sequence of Coronavirus Strains Isolated from Iraqi Patients and their Relationship with some Liver Enzymes and Interleukins

Archives of Razi Institute
مقاله 38، دوره 77، شماره 2، خرداد و تیر 2022، صفحه 809-819    اصل مقاله (683.09 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357089.1967
نویسندگان
R. A Salih1؛ N. S Mohamed* 2؛ A. A Taha3
1Central Public Health Laboratory, Ministry of Health, Baghdad, Iraq
2Pharmacy College, Al- Nahrain University, Baghdad, Iraq
3Department of Applied Science, University of Technology, Baghdad, Iraq
چکیده
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which is a positive-sense single-stranded RNA virus from the genus Betacoronavirus causes COVID-19 (coronavirus disease 2019). According to daily reports issued by the Iraqi Ministry of Health, the SARS-COV-2 was firstly detected in Al-Najaf city in February 2020 and identified in the Central Public Health Laboratory (CPHL) in Baghdad, Iraq. The outcomes of this study were based on 100 nasopharyngeal swaps and venous blood samples from hospitalized patients in Al-Kindy and CPHL. Patients were assigned to five groups (Asymptomatic, Mild, Moderate, Severe, and Deceased) based on disease severity as indicated by World Health Organization (WHO). The positive samples were identified by real-time quantitative polymerase chain reaction (RT-PCR) and subjected to some liver enzyme assays and interleukins measurements, and the correlation with the genetic sequence was determined by Illumina Miseq technology. Liver enzymes levels of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) showed statistically significant differences, especially between the deceased groups. Interleukins (IL-10, IL-18, and TNF- α) significantly differed among groups. This study revealed that three isolates belonging to the original strain isolated from Wuhan (A19) and characterized by their virulence caused severe symptoms and led to admission to isolation hospitals and intensive care units, and the last two isolates of (UK alpha V1) appeared in Iraq in early 2021. These strains which were less virulent than the Wuhan strain spread faster and appear in moderate and asymptomatic patients.
کلیدواژه‌ها
SARS-COV-2؛ Genetic sequence؛ AST؛ ALT؛ LDH؛ TNF- α؛ Il -10؛ Il- 18
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