اخبار و اعلانات

 آمار

تعداد نشریات285
تعداد نشریات سازمان83
تعداد شماره‌ها3,085
تعداد مقالات34,435
تعداد مشاهده مقاله48,796,994
تعداد دریافت فایل اصل مقاله79,286,776
Shakir Alkhafaji, R, Muhsin Khalfa, H, LF Almsaid, H. (1401). Rat Hepatocellular Primary Cells: A Cellular and Genetic Assessment of the Chitosan Nanoparticles-Induced Damage and Cytotoxicity. سامانه مدیریت نشریات علمی, 77(2), 579-584. doi: 10.22092/ari.2022.357103.1974
R Shakir Alkhafaji; H Muhsin Khalfa; H LF Almsaid. "Rat Hepatocellular Primary Cells: A Cellular and Genetic Assessment of the Chitosan Nanoparticles-Induced Damage and Cytotoxicity". سامانه مدیریت نشریات علمی, 77, 2, 1401, 579-584. doi: 10.22092/ari.2022.357103.1974
Shakir Alkhafaji, R, Muhsin Khalfa, H, LF Almsaid, H. (1401). 'Rat Hepatocellular Primary Cells: A Cellular and Genetic Assessment of the Chitosan Nanoparticles-Induced Damage and Cytotoxicity', سامانه مدیریت نشریات علمی, 77(2), pp. 579-584. doi: 10.22092/ari.2022.357103.1974
Shakir Alkhafaji, R, Muhsin Khalfa, H, LF Almsaid, H. Rat Hepatocellular Primary Cells: A Cellular and Genetic Assessment of the Chitosan Nanoparticles-Induced Damage and Cytotoxicity. سامانه مدیریت نشریات علمی, 1401; 77(2): 579-584. doi: 10.22092/ari.2022.357103.1974

Rat Hepatocellular Primary Cells: A Cellular and Genetic Assessment of the Chitosan Nanoparticles-Induced Damage and Cytotoxicity

Archives of Razi Institute
مقاله 9، دوره 77، شماره 2، خرداد و تیر 2022، صفحه 579-584    اصل مقاله (640.72 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357103.1974
نویسندگان
R Shakir Alkhafaji؛ H Muhsin Khalfa* ؛ H LF Almsaid
Department of Biology, Faculty of Sciences, University of Kufa, Kufa, Iraq
چکیده
Chitosan (CH) is a non-toxic vital polymer that is derived naturally from chitin. Due to its anti-bacterial and anti-fungal properties, it has attracted researchers’ attention. The anti-bacterial activity of 1-3 CH is ideal in an acidic medium due to its weak solubility at pH levels higher than 6.5. The type of CH and the degree of its polymerization affect its anti-microbial activity, as well as some of its other chemical and physical properties. The present study was conducted to investigate the damage induced by chitosan nanoparticles (CHNPs) at various concentrations on the cultured rat hepatic cells. The CHNPs were synthesized by the ionotropic gelation of CH with sodium tripolyphosphate anions. Hepatic cells were cultured from tissues freshly isolated from the liver of normal laboratory rats. Cells were allowed to reach a confluence level before the treatment with CHNPs. In total, five different concentrations of CHNPs were used, and cell cytotoxicity was evaluated using the MTT assay. The genetic expression of P2Y1, P2Y2, and P2Y4 purinergic receptors was evaluated on the cellular level using Qualitative Reverse Transcription Polymerase Chain Reaction technique. The primary culture of rat hepatic cells was thoroughly exposed to a range of CHNPs. Under normal conditions, the cells showed normal cellular morphology with clearly defined borders and normal nuclear structure. Apoptotic cellular damage was observed in the cultured hepatic cells when exposed to CHNPs. Moreover, irregular cellular morphology and heavy pigmentation were noticed in the hepatic cells when exposed to a high concentration of CHNPs. Purinergic receptor gene expression indicated an inflammatory response by an increased gene fold change post-exposure to CHNPs. This study concludes that CHNPs have a strong cytotoxic effect on the cultured rat hepatic cells. Overall, CHNPs showed an inhibitory response to hepatic cells evoking a purine receptor-mediated inflammatory response.
کلیدواژه‌ها
Cell culture؛ Chitosan nanoparticles؛ Hepatic
سایر فایل های مرتبط با مقاله
مراجع
  1. Tiyaboonchai W. Chitosan nanoparticles: a promising system for drug delivery. NUJST. 2013;11(3):51-66.
  2. Bhumkar DR, Joshi HM, Sastry M, Pokharkar VB. Chitosan reduced gold nanoparticles as novel carriers for transmucosal delivery of insulin. Pharm Res. 2007;24(8):1415-26.
  3. Regiel-Futyra A, Kus-Liśkiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel Gy, et al. Development of noncytotoxic chitosan–gold nanocomposites as efficient antibacterial materials. ACS Appl Mater Interfaces. 2015;7(2):1087-99.
  4. Md S, Khan RA, Mustafa G, Chuttani K, Baboota S, Sahni JK, et al. Bromocriptine loaded chitosan nanoparticles intended for direct nose to brain delivery: pharmacodynamic, pharmacokinetic and scintigraphy study in mice model. Eur J Pharm Sci. 2013;48(3):393-405.
  5. Kandra P, Kalangi HPJ. Current understanding of synergistic interplay of chitosan nanoparticles and anticancer drugs: merits and challenges. Appl Microbiol Biotechnol. 2015;99(5):2055-64.
  6. Li D, Wang Y, Zhang L, Luo X, Li J, Chen X, et al. Roles of Purinergic Receptor P2Y, G Protein–Coupled 12 in the Development of Atherosclerosis in Apolipoprotein E–Deficient Mice. Arterioscler Thromb Vasc Biol. 2012;32(8):e81-e9.
  7. Alarcón-Vila C, Pizzuto M, Pelegrín P. Purinergic receptors and the inflammatory response mediated by lipids. Curr Opin Pharmacol. 2019;47:90-6.
  8. Albideri A. Histological and cytoarchitectural measurements of human epidermis in different anatomical sites of embryonic, fetal and neonatal Iraqi subjects in Al-Hilla/Iraq Maternity Hospital. People also ask. 2018;10(4):812-8.
  1. Liu S, Zhang H, Duan E. Epidermal development in mammals: key regulators, signals from beneath, and stem cells. Int J Mol Sci. 2013;14(6):10869-95.
  1. Peng H, Hao Y, Mousawi F, Roger S, Li J, Sim JA, et al. Purinergic and store‐operated Ca2+ signaling mechanisms in mesenchymal stem cells and their roles in ATP‐induced stimulation of cell migration. Stem Cells. 2016;34(8):2102-14.
  2. Neary JT, Kang Y, Bu Y, Yu E, Akong K, Peters CM. Mitogenic signaling by ATP/P2Y purinergic receptors in astrocytes: involvement of a calcium-independent protein kinase C, extracellular signal-regulated protein kinase pathway distinct from the phosphatidylinositol-specific phospholipase C/calcium pathway. J Neurosci. 1999;19(11):4211-20.
  1. Allcorn S, Catsicas M, Mobbs P. Developmental expression and self‐regulation of Ca2+ entry via AMPA/KA receptors in the embryonic chick retina. Eur J Neurosci. 1996;8(12):2499-510.
  2. khalfa HM, al-msaid Hl, abood Ah, naji Ma, Hussein Sk, editors. Cellular genetic expression of purinergic receptors in different organs of male rats injected with cyclophosphoamide. AIP Conference Proceedings; 2020: AIP Publishing LLC.
آمار
تعداد مشاهده مقاله: 1,621
تعداد دریافت فایل اصل مقاله: 2,599


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب