اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,074
تعداد مقالات34,301
تعداد مشاهده مقاله47,134,474
تعداد دریافت فایل اصل مقاله78,041,650
Kadhim Mohammed, R. (1401). Investigation of the Role of Virulence Gene in Biofilm Formation of Escherichia coli Obtained from Clinical Specimens in Baghdad. سامانه مدیریت نشریات علمی, 77(2), 915-921. doi: 10.22092/ari.2022.357205.1995
R Kadhim Mohammed. "Investigation of the Role of Virulence Gene in Biofilm Formation of Escherichia coli Obtained from Clinical Specimens in Baghdad". سامانه مدیریت نشریات علمی, 77, 2, 1401, 915-921. doi: 10.22092/ari.2022.357205.1995
Kadhim Mohammed, R. (1401). 'Investigation of the Role of Virulence Gene in Biofilm Formation of Escherichia coli Obtained from Clinical Specimens in Baghdad', سامانه مدیریت نشریات علمی, 77(2), pp. 915-921. doi: 10.22092/ari.2022.357205.1995
Kadhim Mohammed, R. Investigation of the Role of Virulence Gene in Biofilm Formation of Escherichia coli Obtained from Clinical Specimens in Baghdad. سامانه مدیریت نشریات علمی, 1401; 77(2): 915-921. doi: 10.22092/ari.2022.357205.1995

Investigation of the Role of Virulence Gene in Biofilm Formation of Escherichia coli Obtained from Clinical Specimens in Baghdad

Archives of Razi Institute
مقاله 50، دوره 77، شماره 2، خرداد و تیر 2022، صفحه 915-921    اصل مقاله (464.94 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357205.1995
نویسنده
R Kadhim Mohammed*
Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq
چکیده
Several strains of Escherichia coli (E. coli) cause many diseases, including gastrointestinal illness, urinary tract infections, pericarditis, and septicemia. The present study aimed to evaluate the prevalence of the Universal Stress Protein (USP) virulence gene and the level of antibiotic resistance patterns associated with biofilm formation of E. coli in patients with infected burns, wounds, and urinary tract infections. Cases were selected from two hospitals of Al-Yarmouk Educational Hospitals and Baghdad Medical City, Baghdad, Iraq. The clinical specimens were classified as E. coli according to CLSI. The frequency of the USP gene was determined using the PCR technique. The rate of biofilm formation and antibiotic resistance were determined using microplate and agar diffusion methods, respectively. The recorded data on the distribution of E. coli isolates indicated that 33 (66%) of isolates were recovered from females and 17 (34%) of them were obtained from males (P=0.02). The results of the distribution of the isolates indicated that 16 (32%) and 18 (36%) isolates were recovered from 10-20 and 21-30 and 31-40 years old participants, respectively. The recorded data revealed that the highest rate of E. coli isolates was obtained from urine samples while the lowest one was recovered from burn samples (P<0.0001). The frequency of USP gene distribution from all strains was analyzed by the PCR and gel electrophoresis techniques. The results of the PCR test identified the USP gene (toxin gene) at 435 bp. The USP gene was presented in 41 (82%) E. coli isolates of all samples, including 28 isolates (46%) in women and 13 isolates (26%) in men with no significant association. Concerning the distribution due to the age groups, the USP gene was presented in 11 isolates (22%) in the age group of 10-20 years, while 14 (28%) and 16 (32%) isolates in the age groups of (21-30) and (31-40), respectively. Concerning the distribution of samples, the USP gene was presented in 1 isolate (2%) from the burn, 4 isolates (8%) from the wound, and 36 isolates (72%) from the urine. The microtiter plate method was used to evaluate biofilm formation and the results showed that 7 (14%), 28 (56%), and 15 (30%) isolates were weakly, moderately, and strongly adherent, respectively. These results filled the national gap about virulence and antimicrobial resistance of E. coli responsible for several diseases and should be used to improve the management of patients in Baghdad.
کلیدواژه‌ها
Antibiotic Resistance؛ Biofilm؛ E. coli؛ USP Gene
سایر فایل های مرتبط با مقاله
مراجع
  1. Ibrahim AA. Identification of iha and kpsMT virulence genes in Escherichia coli isolates with urinary tract infection in Iraqi patients. Indian J Nat Sci. 2019;52(9):16675-82.
  2. Özdemir F, Arslan S. Molecular Characterization and Biofilm Formation of Escherichia coli from Vegetables. Sakarya Univ J Sci. 2021;25(1):12-21.
  3. Lai YM, Zaw MT, Shamsudin SB, Lin Z. Polymerase chain reaction-restriction fragment length polymorphism method for differentiation of uropathogenic specific protein gene types. J Microbiol Immunol Infect. 2016;49(4):591-4.
  4. Saekhow P, Sriphannam C. Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli strains in dairy farm wastewater in Chiang Mai. Vet Integr Sci. 2021;19(3):349-62.
  5. Nipic D, Podlesek Z, Budic M, Crnigoj M, Zgur-Bertok D. Escherichia coli uropathogenic-specific protein, Usp, is a bacteriocin-like genotoxin. J Infect Dis. 2013;208(10):1545-52.
  6. Nikzad M, Mirnejad R, Babapour E. Evaluation of Antibiotic Resistance and Biofilm Formation Ability Uropathogenic E. coli (UPEC) Isolated From Pregnant Women in Karaj. Iran J Med Microbiol. 2021;15(2):195-211.
  7. Zhanel GG, Hisanaga TL, Laing NM, DeCorby MR, Nichol KA, Weshnoweski B, et al. Antibiotic resistance in Escherichia coli outpatient urinary isolates: final results from the North American Urinary Tract Infection Collaborative Alliance (NAUTICA). Int J Antimicrob Agents. 2006;27(6):468-75.
  8. Humphries RM, Ambler J, Mitchell SL, Castanheira M, Dingle T, Hindler JA, et al. CLSI Methods Development and Standardization Working Group Best Practices for Evaluation of Antimicrobial Susceptibility Tests. J Clin Microbiol. 2018;56(4).
  9. Dong G, Li J, Chen L, Bi W, Zhang X, Liu H, et al. Effects of sub-minimum inhibitory concentrations of ciprofloxacin on biofilm formation and virulence factors of Escherichia coli. Braz J Infect Dis. 2019;23(1):15-21.
  10. Valadbeigi H, Esmaeeli E, Ghafourian S, Maleki A, Sadeghifard N. Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections. Infect Disord Drug Targets. 2019;19(3):322-6.
  11. Vollmerhausen TL, Ramos NL, Gundogdu A, Robinson W, Brauner A, Katouli M. Population structure and uropathogenic virulence-associated genes of faecal Escherichia coli from healthy young and elderly adults. J Med Microbiol. 2011;60(5):574-81.
  12. Foxman B, Brown P. Epidemiology of urinary tract infections: transmission and risk factors, incidence, and costs. Infect Dis Clin. 2003;17(2):227-41.
  13. Foxman B. The epidemiology of urinary tract infection. Nat Rev Urol. 2010;7(12):653-60.
  14. Wiles TJ, Kulesus RR, Mulvey MA. Origins and virulence mechanisms of uropathogenic Escherichia coli. Exp Mol Pathol. 2008;85(1):11-9.
  1. Peerayeh SN, Navidinia M, Fallah F, Bakhshi B, Jamali J. Pathogenicity determinants and epidemiology of uropathogenic E. coli (UPEC) strains isolated from children with urinary tract infection (UTI) to define distinct pathotypes. Biom Res. 2018;29(10):2035-43.
  2. Paniagua-Contreras GL, Monroy-Perez E, Rodriguez-Moctezuma JR, Dominguez-Trejo P, Vaca-Paniagua F, Vaca S. Virulence factors, antibiotic resistance phenotypes and O-serogroups of Escherichia coli strains isolated from community-acquired urinary tract infection patients in Mexico. J Microbiol Immunol Infect. 2017;50(4):478-85.
  3. Al-Shamari RK, Al-Khteeb, Sumaya N. Molecular Characterization Aminoglycosids Resistance Pseudomonas aeruginosa. Iraqi J Sci. 2016;57(2):1150-7.
  4. Polse R, Yousif S, Assafi M. Prevalence and antimicrobial susceptibility patterns of uropathogenic E. coli among people in Zakho, Iraq. Int J Res Med Sci. 2016;4(4):1219-23.
  5. Goller CC. Regulation of β-1, 6-N-acetyl-D-glucosamine production and Escherichia coli biofilm formation: Emory University; 2008.
  6. Bohl LP, Isaac P, Breser ML, Orellano MS, Correa SG, Tolosa de Talamoni NG, et al. Interaction between bovine mammary epithelial cells and planktonic or biofilm Staphylococcus aureus: The bacterial lifestyle determines its internalization ability and the pathogen recognition. Microb Pathog. 2021;152:104604.
آمار
تعداد مشاهده مقاله: 1,574
تعداد دریافت فایل اصل مقاله: 2,580


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب