| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,073 |
| تعداد مقالات | 34,300 |
| تعداد مشاهده مقاله | 47,126,498 |
| تعداد دریافت فایل اصل مقاله | 78,033,437 |
Effect of Epididymal Spermatozoa of Local Iraqi Goat on in vitro Fertilization and Evolution of Embryos | ||
| Archives of Razi Institute | ||
| مقاله 17، دوره 77، شماره 2، خرداد و تیر 2022، صفحه 641-646 اصل مقاله (366.3 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.357269.2008 | ||
| نویسندگان | ||
| S Shakir Hammoud* ؛ M Adil Jebur | ||
| Al-Farabi University College, Baghdad, Iraq | ||
| چکیده | ||
| In vitro fertilization (IVF) has been considered one of the greatest improvements in livestock science and production. Epididymal spermatozoon is a valuable gamete source obtained from slaughtered animals, and it has great importance in livestock IVF. This study aimed to create caprine embryos in Baghdad using local Iraqi goats. Ovaries were obtained from 50 female local goats at the Al-shu'alah abattoir. The ovaries were transported to the laboratory in physiological saline solution at 38°C. After oocyte recovery from the mature antral follicle, the oocytes with grades A or B were used for in vitro maturation (IVM). Moreover, 10 testicles were obtained from slaughtered adult goats, and the sperm was recovered from the caudal epididymis. All the stage-specific culture mediums were incubated at 39°C with 5% CO2 and 90% relative humidity. In this study, embryonic development on a 24-h basis was evaluated and in vitro culture media was replaced 50% of the volume every day for nine days with fresh media until an enlarged or hatched blastocyst appeared. The recorded data showed that the mean size of the large follicles was 6.23±1.34 mm, while the small follicles were in the mean size of 3.10±0.62. On the other hand, the results showed a significant increase in the oocyte recovery rate from the large size follicle (81.22%), compared to the small size follicle (62.67%). The recorded data showed that the total number of retrieved oocytes with grades A and B was 290 (79.5%) (Table 1). The recorded data showed that 50.1% of these oocyte cohorts had reached metaphase II (145/290). Furthermore, 45.45% of the recovered oocytes by aspiration from large follicles were developed to the blastocyst stage, compared to 25% of recovered oocytes from the small follicles reaching the same developmental stage (blastocyst). On the other hand, the recorded data showed that the oocyte recovery method had a great influence on the oocyte competence regardless of the follicle size. The results revealed that the number of recovered oocytes by aspiration was greater than slicing. In conclusion, the results approved that the slicing method could be considered a safe and more efficient method in oocyte recovery from the slaughtered animals, compared to the aspiration method. On the other hand, according to the findings, the epididymal spermatozoa from local Iraqi goats are a reliable source of oocytes for IVF and in vitro conversion. | ||
| کلیدواژه‌ها | ||
| Dead؛ Embryo Production؛ Epididymis؛ In Vitro؛ Local Iraqi Goat | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
1. Dong Q, Rodenburg SE, Huang C, Vandevoort CA. Cryopreservation of Rhesus Monkey (Macaca mulatta) Epididymal Spermatozoa Before and After Refrigerated Storage. J Androl. 2008;29(3):283-92.
2. Hafez ESE, Hafez B. Folliculogenesis, Egg Maturation, and Ovulation. Reproduction in Farm Animals. 2000. p. 68-81.
3. Brüssow K, Torner H, Rátky J, Hunter M, Nürnberg G. Ovum pick up in swine: the influence of aspiration vacuum pressure on oocyte recovery from preovulatory follicles. Acta Vet Hung. 1997;45:189-96.
4. Hoque SM, Kabiraj SK, Khandoker MY, Mondai A, Tareq K. Effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation and fertilization of goat oocytes. Afr J Biotechnol. 2011;10(45):9177-88.
5. Wani N, Wani G, Khan M, Salahudin S. Effect of oocyte harvesting techniques on in vitro maturation and in vitro fertilization in sheep. Small Rumin Res. 2000;36(1):63-7.
6. Zeinoaldini S, Jafari Z, Sarmast F, Torbati E, Davachi ND. Different harvesting techniques used in ovine in vitro embryo production. Scimetr. 2013;1(1).
7. Galli C, Duchi R, Crotti G, Turini P, Ponderato N, Colleoni S, et al. Bovine embryo technologies. Theriogenology. 2003;59(2):599-616.
8. Liu W, Yin Y, Long X, Luo Y, Jiang Y, Zhang W, et al. Derivation and characterization of human embryonic stem cell lines from poor quality embryos. J Genet Genomics. 2009;36(4):229-39.
9. Davachi ND, Shahneh AZ, Kohram H, Zhandi M, Dashti S, Shamsi H, et al. In vitro ovine embryo production: the study of seasonal and oocyte recovery method effects. Iran Red Crescent Med J. 2014;16((9.
10. Davachi ND, Kohram H, Zainoaldini S. Cumulus cell layers as a critical factor in meiotic competence and cumulus expansion of ovine oocytes. Small Rumin Res. 2012;102(1):37-42.
11. Davachi ND, Shahneh AZ, Kohram H, Zhandi M, Shamsi H, Hajiyavand AM, et al. Differential influence of ampullary and isthmic derived epithelial cells on zona pellucida hardening and in vitro fertilization in ovine. Reprod Biol. 2016;16(1):61-9.
12. Kharche S, Goel P, Kumar Jha B, Goel A, Jindal S. Factors influencing in-vitro embryo production efficiency of caprine oocytes: A review. Indian J Anim Sci. 2011;81(4):344.
13. Farag I, Girgis S, Hassan N, Khalil W, Sakr A, Ali N. Effect of protein additives on in vitro maturation of Egyptian sheep oocytes with reference to seasonal variation effects on yield and quality of oocytes. J Am Sci. 2010;6(10):588-9.
14. Majeed A, Saied I, Al-Saigh M. Effect of collection techniques on recovery, and in vitro maturation of Black Iraqi Goats oocytes. Al-Anbar J Vet Sci. 2011;4(2):178-81.
15. Hafez ESE, Hafez B. Reproductive Cycles. Reproduction in Farm Animals. 2000. p. 55-67.
16. Wani N. In vitro maturation and in vitro fertilization of sheep oocytes. Small Rumin Res. 2002;44(2):89-95.
17. Younis A, Zuelke K, Harper K, Oliveira M, Brackett B. In vitro fertilization of goat oocytes. Biol Reprod. 1991;44(6):1177-82.
18. Abdullah R, Liow S, Rahman A, Chan W, Wan-Khadijah W, Ng S. Prolonging the interval from ovarian hyperstimulation to laparoscopic ovum pick-up improves oocyte yield, quality, and developmental competence in goats. Theriogenology. 2008;70(5):765-71.
19. Lonergan P, Monaghan P, Rizos D, Boland M, Gordon I. Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization, and culture in vitro. Mol Reprod Dev. 1994;37(1):48-53.
20. Wang Z, Xu Z, Yu S. Effects of oocyte collection techniques and maturation media on. Vitro. 2007.
21. Khatun M, Bhuiyan MMU, Ahmed JU, Haque A, Rahman MB, Shamsuddin M. In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes. J Vet Sci. 2011;12(1):75-82.
22. Sogorescu E, Zamfierescu S, Anghel AH, Dorina N. The influence of new media on the developmental competence of goat and sheep oocytes. Rom Biotechnol Lett. 2010;15(3):19-25. | ||
|
آمار تعداد مشاهده مقاله: 1,745 تعداد دریافت فایل اصل مقاله: 2,660 |
||
