اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها3,020
تعداد مقالات33,742
تعداد مشاهده مقاله44,988,900
تعداد دریافت فایل اصل مقاله47,434,364
Shnewer Mahdi Al-Turfi, Z, Al-Hadrawy, S. M. J, Abadi Mohammed, J, Chasib Jabal, B. (1401). Evaluation of the Effect of Alcoholic Extract of Laurus Nobilis Leaves on Blood Biochemical Parameters and Histological Changes in the Liver and Kidney among Female Wistar Rats Treated with Depakene (Sodium Valproate). سامانه مدیریت نشریات علمی, 77(3), 981-989. doi: 10.22092/ari.2022.357272.2011
Z Shnewer Mahdi Al-Turfi; S. M. J Al-Hadrawy; J Abadi Mohammed; B Chasib Jabal. "Evaluation of the Effect of Alcoholic Extract of Laurus Nobilis Leaves on Blood Biochemical Parameters and Histological Changes in the Liver and Kidney among Female Wistar Rats Treated with Depakene (Sodium Valproate)". سامانه مدیریت نشریات علمی, 77, 3, 1401, 981-989. doi: 10.22092/ari.2022.357272.2011
Shnewer Mahdi Al-Turfi, Z, Al-Hadrawy, S. M. J, Abadi Mohammed, J, Chasib Jabal, B. (1401). 'Evaluation of the Effect of Alcoholic Extract of Laurus Nobilis Leaves on Blood Biochemical Parameters and Histological Changes in the Liver and Kidney among Female Wistar Rats Treated with Depakene (Sodium Valproate)', سامانه مدیریت نشریات علمی, 77(3), pp. 981-989. doi: 10.22092/ari.2022.357272.2011
Shnewer Mahdi Al-Turfi, Z, Al-Hadrawy, S. M. J, Abadi Mohammed, J, Chasib Jabal, B. Evaluation of the Effect of Alcoholic Extract of Laurus Nobilis Leaves on Blood Biochemical Parameters and Histological Changes in the Liver and Kidney among Female Wistar Rats Treated with Depakene (Sodium Valproate). سامانه مدیریت نشریات علمی, 1401; 77(3): 981-989. doi: 10.22092/ari.2022.357272.2011

Evaluation of the Effect of Alcoholic Extract of Laurus Nobilis Leaves on Blood Biochemical Parameters and Histological Changes in the Liver and Kidney among Female Wistar Rats Treated with Depakene (Sodium Valproate)

Archives of Razi Institute
مقاله 8، دوره 77، شماره 3، مرداد و شهریور 2022، صفحه 981-989    اصل مقاله (1.09 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357272.2011
نویسندگان
Z Shnewer Mahdi Al-Turfi* 1؛ S. M. J Al-Hadrawy2؛ J Abadi Mohammed1؛ B Chasib Jabal3
1University of Kufa, Faculty of Education for Girls, Department of Biology, Kufa, Iraq
2University of Kufa, Faculty of Science, Department of Biology, Kufa, Iraq
3The Islamic University, College of Medical Technology, Medical Laboratory
چکیده
This study aimed to evaluate the effects of Laurus Nobilis (Bay leaves) alcoholic extract on glucose, hemoglobin A1c (HbA1c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine, and urea levels; moreover, it was attempted to examine the histological changes induced in the liver and kidney among female albino rats treated with Depakene (Sodium Valproate). The L. nobilis leaves were dried in the shade, and they were then ground in mechanical processing. The resulting substance (250 gm) was processed in 70% ethanol for 24 h using a Soxhlet extractor at 45°C. Before being measured, the extract was concentrated in vacuo and stored in a vacuum desiccator until the elimination of all the solvents. In total, 20 female adult Wistar rats (230-250 g) were bred in the Animal House Lab at the University of Kufa, Faculty of Education for Girls, Kufa, Iraq. These animals were randomly divided into four groups (n=5), housed in a typical laboratory setting, and given a standard diet and water. Each animal received the treatments intraperitoneally for 30 days. The experimental groups were designed as follows: group 1 (the control) was given only physiological saline solution; group 2 received alcoholic extract of L. nobilis leaves at a dose of 150 mg/kg BW; group 3 received Depakene (Sodium Valproate) at a dose of 500 mg/kg BW; and group 4 received alcoholic extract+Depakene at a dose of 150 mg/kg BW and 500 mg/kg BW. The animals were euthanized following anaesthesia 24 h after the last day of the experiment. Heart blood samples were gathered in gel tubes, the serum was then centrifuged for 15 min at 3000 rpm to measure the biochemical parameter levels, which included glucose, HbA1C, ALT, AST, creatinine, and urea. The liver and kidney organs were removed and placed in a 10% formaldehyde solution instantly. Following fixation, they were processed as usual before being embedded in paraffin for histological analysis. Morphological changes were assessed using hematoxylin and eosin staining techniques. The recorded data showed a major drop (P<0.05) in blood glucose and HbA1c levels in group 2 which was given ethanol extract, compared to the other groups. Interestingly, the level of blood glucose and HbA1c levels reduced significantly in group 4, which was given L. nobilis+Depakene, compared to the control and the animals treated with only Depakene. Moreover, the results showed a major rise (P<0.05) in the liver enzyme among the animals treated with Depakene, compared to other groups. On the other hand, the recorded data showed a substantial drop (P<0.05) in creatinine levels in the animals treated with L. nobilis leaves extract (group 2) and group 4, compared to group 3 and the control group, respectively. However, no changes were recorded in the case of urea levels among the groups. Finally, the findings of this study showed that the ethanol extract of L. nobilis leaves was effectively reduced the adverse effects of Depakene. On the other hand, it had a significant effect on the reduction of blood glucose.
کلیدواژه‌ها
Depakene drug؛ Glucose level؛ Laurus Nobilis leaves؛ Liver and kidney function
مراجع
  1. American Diabetes A. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2013;36(1):67-74.
  2. Yu T, Robotham JL, Yoon Y. Increased production of reactive oxygen species in hyperglycemic conditions requires dynamic change of mitochondrial morphology. Proc Natl Acad Sci U S A. 2006;103(8):2653-8.
  3. Nandhini LP, Kamalanathan S, Sahoo J. Definition, Diagnostic Criteria, Screening, Diagnosis, and Classification of Diabetes and Categories of Glucose Intolerance. In: Rodriguez-Saldana J, editor. The Diabetes Textbook: Clinical Principles, Patient Management and Public Health Issues. Cham: Springer International Publishing; 2019. p. 71-85.
  4. Rahimi M. A review: antidiabetic medicinal plants used for diabetes mellitus. Bull Environ Pharmacol Life Scienes. 2015;4(2):163-80.
  5. Guyton A, Hall J. Textbook of medical physiology, 11th. Elsevier Inc.; 2006.
  6. Singh A, Sharma O. Clinical Biochemistry of Hepatotoxicity. J Clinic Toxicol. 2011:1-19.
  7. Yakubu MT, Adebayo OJ, Egwim EC, Owoyele VB. Increased liver alkaline phosphatase and aminotransferase activities following administration of ethanolic extract of Khaya senegalensis stem bark to rats. Biokemistri. 2005;17:27-32.
  8. Guyton AC, Hall JE. Textbook of Medical physiology 14th, editor: Elsevier Inc; 2021.
  9. Qutob FT, . Medical plants:Planting and Component.1981.
  10. Al-Janabi B. Herbs and Seasoning.1988.
  11. Chahal K, Kaur M, Bhardwaj U, Singla N, Kaur A, Kaur M, et al. A review on chemistry and biological activities of Laurus nobilis L. essential oil. J pharmacogn. phytochem. 2017;6(4):1153-61.
  12. Yahyaa M, Berim A, Isaacson T, Marzouk S, Bar E, Davidovich-Rikanati R, et al. Isolation and Functional Characterization of Carotenoid Cleavage Dioxygenase-1 from Laurus nobilis L. (Bay Laurel) Fruits. J Agric Food Chem. 2015;63(37):8275-82.
  13. Elena T, Zhilyakova* OO, Novikov DI, Pisarev AY, Malyutina NN, Boyko. Studying The Polyphenolic Structure Of Laurus Nobilis L. Leaves. Indo Am J Pharm Sci. 2017;04:3066–74.
  14. Mansour O, Darwish M, Ismail G, Douba ZA-A, Ismaeel A, Eldair K. Review Study on the Physiological Properties and Chemical Composition of the Laurus nobilis. 2018;5.
  15. TADA H, TAKEDA K, Bulletin P. Sesquiterpenes of lauraceae plants. IV. Germacranolides from Laurus nobilis L. Chem Pharm Bull 1976;24(4):667-71.
  16. Matsuda H, Kagerura T, Toguchida I, Ueda H, Morikawa T, Yoshikawa M. Inhibitory effects of sesquiterpenes from bay leaf on nitric oxide production in lipopolysaccharide-activated macrophages: Structure requirement and role of heat shock protein induction. Life Sci. 2000;66(22):2151-7.
  17. Santos FA, Rao VSN. Antiinflammatory and antinociceptive effects of 1,8-cineole a terpenoid oxide present in many plant essential oils. Phytother Res. 2000;14(4):240-4.
  18. Silva N, Fernandes Júnior A. Biological properties of medicinal plants: a review of their antimicrobial activity. J Venom Anim Toxins Incl Trop Dis.2010;16(3):402-13.
  19. Jian Ik, Alzobidy AMH. Study the effect of bay leaf extraction the some biochemical parameters in diabetic male rat Iinduced by alloxan. Bas J Vet Res. 2018;17(1).
  20. Mohammed RR, Omer AK, Yener Z, Uyar A, Ahmed AK. Biomedical effects of Laurus nobilis L. leaf extract on vital organs in streptozotocin-induced diabetic rats: Experimental research. Ann Med Surg. 2021;61:188-97.
  21. Rishan AM, Aziz RA, Al-Temimi SS. Determine the chemical content of Bay (Laurus nobilis L.) leaves extract and its effectivenss against some bacterial species: Determine the chemical content of Bay (Laurus nobilis L.) leaves extract and its effectivenss against some bacterial species. Iraq J Mark Res Consum Protect. 2016;8(1):281-301.
  22. Fagundes SBR. Ácido Valpróico:: revisão. Revista Neurociências. 2008;16(2):130-6.
  23. Gayam V, Mandal AK, Khalid M, Shrestha B, Garlapati P, Khalid M. Valproic acid induced acute liver injury resulting in hepatic encephalopathy- a case report and literature review. J Community Hosp Intern Med Perspect. 2018;8(5):311-4.
  24. Ibrahim. Evaluation of Hepatotoxicity of Valproic acid in albino mice , Histological and Histoistochemical studies. Life Sci J.2012;9(4) :153-159
  25. Ghodke-Puranik Y, Thorn CF, Lamba JK, Leeder JS, Song W, Birnbaum AK, et al. Valproic acid pathway: pharmacokinetics and pharmacodynamics. Pharmacogenet Genomics. 2013;23(4):236-41.
  26. Chang TK, Abbott FS. Oxidative stress as a mechanism of valproic acid-associated hepatotoxicity.
    Drug Metab Rev. 2006;38(4):627-39.
  27. A M, Avanapu SR. Comparative Hepatoprotective activity of Selected Indian Medicinal Plants. Glob J Med Res. 2013;13:15-21.
  28. Alchalabi S, Majeed D, Jasim A, Al-Azzawi K. Effect of ethanolic extract of Bay leaves on blood sugar level. Ann Trop Med Public Health. 2020;23.
  29. Dias MI, Barros L, Duenas M, Alves RC, Oliveira MB, Santos-Buelga C, et al. Nutritional and antioxidant contributions of Laurus nobilis L. leaves: would be more suitable a wild or a cultivated sample? Food Chem. 2014;156:339-46.
  30. Ham A-R, Shin J-H, Oh K-B, Lee S-J, Nam K-W, Koo U, et al. . Neuroprotective Effect of the n-Hexane Extracts of Laurus nobilis L. in Models of Parkinson’s Disease. Biomol Ther. 2011;19: 118–25.
  31. Vardapetyan H, Tiratsuyan S, Hovhannisyan  A, Rukhkyan  M, Hovhannisyan D. Phytochemical composition and biological activityof Laurus nobilis L. leaves collected from two regions of South Caucasus. J Exp Biol Agric Sci. 2013;1(45-51).
  32. G G, Tiratsuyan S, Kazarian S, H V. Effect of Laurus nobilis extract on the functioning of liver against CCL4 induced toxicity. J Exp Biol Agric Sci. 2015;3:174-83.
  33. Kaurinovic B, Popovic M, Vlaisavljevic S. In vitro and in vivo effects of Laurus nobilis L. leaf extracts. Molecules. 2010;15(5):3378-90.
  34. Casamassima D, Chiosi F, Vizzarri F, Palazzo M, Costagliola C. The effect of Laurus nobilis on the blood and lenses antioxidant activity in rabbit under fat-enriched diet. Physiol Res. 2017;66(2):325-33.
  35. Patrakar R, Mansuriya M, Patil PP, editors. Phytochemical and Pharmacological Review on Laurus Nobilis.2012.
  36. Ravindran CA, Murugaiyah V, Kok-Khiang P, Xavior R. Hepatoprotective activity of leaf of methanol extract of Laurus nobilis.L against paracetamol induced hepatotoxicity in rats. Asian J Pharm Clin Res. 2013;6:153-7.
  37. Mittler R, Vanderauwera S, Suzuki N, Miller G, Tognetti VB, Vandepoele K, et al. ROS signaling: the new wave? Trends Plant Sci. 2011;16(6):300-9.
  38. Khan S, Shakoor K, Jan M, Khattak A, Shah S. Study of Histopathologic changes in the Liver of Albino Rats, Induced by Toxic doses of Valproic Acid. Gomal J Med Sci. 2005;3:15-8.
آمار
تعداد مشاهده مقاله: 1,837
تعداد دریافت فایل اصل مقاله: 1,731


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب