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Nama, M. A. (1401). Gene Silencing of Toll-like Receptor 2 Gene Expression as a Tactic to Control Mycobacterium Tuberculosis and Granuloma Formation. سامانه مدیریت نشریات علمی, 77(2), 827-834. doi: 10.22092/ari.2022.357152.1984
M. A Nama. "Gene Silencing of Toll-like Receptor 2 Gene Expression as a Tactic to Control Mycobacterium Tuberculosis and Granuloma Formation". سامانه مدیریت نشریات علمی, 77, 2, 1401, 827-834. doi: 10.22092/ari.2022.357152.1984
Nama, M. A. (1401). 'Gene Silencing of Toll-like Receptor 2 Gene Expression as a Tactic to Control Mycobacterium Tuberculosis and Granuloma Formation', سامانه مدیریت نشریات علمی, 77(2), pp. 827-834. doi: 10.22092/ari.2022.357152.1984
Nama, M. A. Gene Silencing of Toll-like Receptor 2 Gene Expression as a Tactic to Control Mycobacterium Tuberculosis and Granuloma Formation. سامانه مدیریت نشریات علمی, 1401; 77(2): 827-834. doi: 10.22092/ari.2022.357152.1984

Gene Silencing of Toll-like Receptor 2 Gene Expression as a Tactic to Control Mycobacterium Tuberculosis and Granuloma Formation

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مقاله 40، دوره 77، شماره 2، خرداد و تیر 2022، صفحه 827-834    اصل مقاله (632.27 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357152.1984
نویسنده
M. A Nama*
Microbiology, Basic Medical Science Department, Nursing College, Iraq
چکیده
Mycobacterium tuberculosis (MT) is the causative agent of tuberculosis (TB) in humans. Tuberculosis is one of the top 10 causes of mortality worldwide, resulting in 1.8 million deaths and 10.4 million new cases in 2016. Understanding the fundamental features of MT biology is critical to the eradication of MT in the future. Due to the increasing frequency of antimicrobial treatment resistance and problems in vaccine development, the pathogenesis of TB for its survival and growth is highly dependent on host lipids and stimulated-lipid droplets formation. Toll-like receptor 2 (TLR2) forms heterophilic dimers with TLR1 and TLR6, therefore, recognizing many MT components. Both of these receptors identify the invading antigen and activate downstream protein kinases. Some studies demonstrated that the cyclooxygenase-2 (COX-2) promoter-driven gene expression includes connecting sites for transcription factors, such as nuclear factor-kappa B, CREB, NFAT, and c/EBPβ. The current study aimed to investigate the role of the TLR2 receptor in positively regulating prostaglandin E2 production in M. bovis (BCG) infected macrophages in vivo using a human monocytic cell line THP-1. Our results revealed that MT infection triggers a time-dependent increase in COX-2 expression via pathways involving TLR2 receptor activation and enhances COX-2 expression, leading to an increase in lipid droplet formation and suppression of macrophage activation.
کلیدواژه‌ها
BCG؛ THP-1 cells model؛ PPARγ؛ TLR2 siRNA knockdown
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مراجع
  1. Zhang Z, Sheng H, Shao J, Beauchamp RD, DuBois RN. Posttranscriptional regulation of cyclooxygenase-2 in rat intestinal epithelial cells. Neoplasia. 2000;2(6):523-30.
  2. Ozato K, Tsujimura H, Tamura T. Toll-Like Receptor Signaling and Regulation of Cytokine Gene Expression in the Immune System. Biotechniques. 2002;33(4):66-75.
  3. Wang L, Zuo M, Chen H, Liu S, Wu X, Cui Z, et al. Mycobacterium tuberculosis Lipoprotein MPT83 Induces Apoptosis of Infected Macrophages by Activating the TLR2/p38/COX-2 Signaling Pathway. J Immunol. 2017;198(12):4772-80.
  4. LaPointe MC, Isenovic E. Interleukin-1beta regulation of inducible nitric oxide synthase and cyclooxygenase-2 involves the p42/44 and p38 MAPK signaling pathways in cardiac myocytes. Hypertension. 1999;33(1 Pt 2):276-82.
  5. Xiong W, Wen Q, Du X, Wang J, He W, Wang R, et al. Novel Function of Cyclooxygenase-2: Suppressing Mycobacteria by Promoting Autophagy via the Protein Kinase B/Mammalian Target of Rapamycin Pathway. J Infect Dis. 2018;217(8):1267-79.
  6. Shinohara T, Pantuso T, Shinohara S, Kogiso M, Myrvik QN, Henriksen RA, et al. Persistent inactivation of macrophage cyclooxygenase-2 in mycobacterial pulmonary inflammation. Am J Respir Cell Mol Biol. 2009;41(2):146-54.
  7. Almeida PE, Carneiro AB, Silva AR, Bozza PT. PPARgamma Expression and Function in Mycobacterial Infection: Roles in Lipid Metabolism, Immunity, and Bacterial Killing. PPAR Res. 2012;2012:383829.
  8. Faridgohar M, Nikoueinejad H. New findings of Toll-like receptors involved in Mycobacterium tuberculosis infection. Pathog Glob Health. 2017;111(5):256-64.
  9. Liu Y, Wang Y, Yamakuchi M, Isowaki S, Nagata E, Kanmura Y, et al. Upregulation of toll-like receptor 2 gene expression in macrophage response to peptidoglycan and high concentration of lipopolysaccharide is involved in NF-kappa b activation. Infect Immun. 2001;69(5):2788-96.
  10. Lin PL, Plessner HL, Voitenok NN, Flynn JL. Tumor necrosis factor and tuberculosis. J Investig Dermatol Symp Proc. 2007;12(1):22-5.
  11. Flo TH, Halaas O, Torp S, Ryan L, Lien E, Dybdahl B, et al. Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol. 2001;69(3):474-81.
  12. Huang Z-F, Massey JB, Via DP. Differential regulation of cyclooxygenase-2 (COX-2) mRNA stability by interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in human in vitro differentiated macrophages. Biochem Pharmacol. 2000;59(2):187-94.
  13. Wu MY, Lu JH. Autophagy and Macrophage Functions: Inflammatory Response and Phagocytosis. Cells. 2019;9(1).
  1. Chin MS, Nagineni CN, Hooper LC, Detrick B, Hooks JJ. Cyclooxygenase-2 gene expression and regulation in human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 2001;42(10):2338-46.
  2. Kang YJ, Mbonye UR, DeLong CJ, Wada M, Smith WL. Regulation of intracellular cyclooxygenase levels by gene transcription and protein degradation. Prog Lipid Res. 2007;46(2):108-25.
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