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Salehi-Najafabadi, Z, Goudarzi, H. R, Sajadi, M. (1400). Evaluation of in vivo Lethality and in vitro Cytotoxic Effect of Odontobuthus bidentatus Scorpion Venom. سامانه مدیریت نشریات علمی, 77(1), 29-36. doi: 10.22092/ari.2021.353302.1595
Z Salehi-Najafabadi; H. R Goudarzi; M Sajadi. "Evaluation of in vivo Lethality and in vitro Cytotoxic Effect of Odontobuthus bidentatus Scorpion Venom". سامانه مدیریت نشریات علمی, 77, 1, 1400, 29-36. doi: 10.22092/ari.2021.353302.1595
Salehi-Najafabadi, Z, Goudarzi, H. R, Sajadi, M. (1400). 'Evaluation of in vivo Lethality and in vitro Cytotoxic Effect of Odontobuthus bidentatus Scorpion Venom', سامانه مدیریت نشریات علمی, 77(1), pp. 29-36. doi: 10.22092/ari.2021.353302.1595
Salehi-Najafabadi, Z, Goudarzi, H. R, Sajadi, M. Evaluation of in vivo Lethality and in vitro Cytotoxic Effect of Odontobuthus bidentatus Scorpion Venom. سامانه مدیریت نشریات علمی, 1400; 77(1): 29-36. doi: 10.22092/ari.2021.353302.1595

Evaluation of in vivo Lethality and in vitro Cytotoxic Effect of Odontobuthus bidentatus Scorpion Venom

Archives of Razi Institute
مقاله 6، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 29-36    اصل مقاله (500.81 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.353302.1595
نویسندگان
Z Salehi-Najafabadi* 1؛ H. R Goudarzi1؛ M Sajadi2
1Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2Department of Biology, Tofigh Daru Research and Engineering Company, Tehran, Iran
چکیده
The results of numerous studies have revealed that some deadly scorpion venoms are composed of various bioactive molecules that have significant cytotoxic effects on cancer cells. In this study, the in vivo lethality and cytotoxic effect of Odontobuthus bidentatus venom were evaluated in different cancer cell lines. Through MTT assay, the cytotoxic effects of O. bidentatus scorpion venom were analyzed on the MCF-7, A549, AGS, HepG2, and Ht-29 cancer cell lines and Hu02 normal cells. To this end, six venom fractions were obtained through a Sephadex G-50 column, and the cytotoxic effects of isolated fractions were evaluated on A549 lung cancer cells.  The median lethal dose of O. bidentatus scorpion venom was determined at 0.73 mg/kg by intravenous administration of different venom doses in male BALB/c mice according to the Spearman-Karber method. The O. bidentatus scorpion whole venom had a significant cytotoxic effect on MCF-7, A549, and AGS cells. The treatment of A549 cells with various concentrations of fraction F1 showed that this fraction significantly induced growth inhibitory effect on the cells in a dose-dependent manner, compared to untreated cells.
کلیدواژه‌ها
Odontobuthus bidentatus venom؛ Cytotoxic effects؛ LD50؛ Cancer cells
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مراجع
  1. Goudarzi HR, Salehi Najafabadi Z, Movahedi A, Noofeli M. Bradykinin-Potentiating Factors of Venom from Iranian Medically Important Scorpions. Arch Razi Inst. 2019;74(4):385-94.
  2. Ortiz E, Gurrola GB, Schwartz EF, Possani LD. Scorpion venom components as potential candidates for drug development. Toxicon. 2015;93:125-35.
  3. Tobassum S, Tahir HM, Arshad M, Zahid MT, Ali S, Ahsan MM. Nature and applications of scorpion venom: an overview. Toxin Rev. 2020;39(3):214-25.
  4. Ghosh A, Roy R, Nandi M, Mukhopadhyay A. Scorpion Venom-Toxins that Aid in Drug Development: A Review. Int J Pept Res Ther. 2019;25(1):27-37.
  5. Zhang XY, Zhang PY. Scorpion venoms in gastric cancer. Oncol Lett. 2016;12(5):3683-6.
  6. Díaz-García A, Ruiz-Fuentes JL, Frión-Herrera Y, Yglesias-Rivera A, Garlobo YR, Sánchez HR, et al. Rhopalurus junceus scorpion venom induces antitumor effect in vitro and in vivo against a murine mammary adenocarcinoma model. Iran J Basic Med Sci. 2019;22(7):759-65.
  7. Ding J, Chua PJ, Bay BH, Gopalakrishnakone P. Scorpion venoms as a potential source of novel cancer therapeutic compounds. Exp Biol Med. 2014;239(4):387-93.
  8. Nagai H, Kim YH. Cancer prevention from the perspective of global cancer burden patterns. J Thorac Dis. 2017;9(3):448-51.
  9. Arruebo M, Vilaboa N, Saez-Gutierrez B, Lambea J, Tres A, Valladares M, et al. Assessment of the evolution of cancer treatment therapies. Cancers. 2011;3(3):3279-330.
  10. Li L, Huang J, Lin Y. Snake Venoms in Cancer Therapy: Past, Present and Future. Toxins. 2018;10(9).
  11. Chaisakul J, Hodgson WC, Kuruppu S, Prasongsook N. Effects of Animal Venoms and Toxins on Hallmarks of Cancer. J Cancer. 2016;7(11):1571-8.
  12. Mishal R, Tahir M, Zafar K, Arshad M. Anti-cancerous applications of scorpion venom. Asian J Pharm Biol Res. 2013;4:356-60.
  13. Raposo C. Scorpion and spider venoms in cancer treatment: state of the art, challenges, and perspectives. J Clin Transl Res. 2017;3(2):233-49.
14.          Haghani A, Khoobdel M, Dehghani R, Adibzadeh A, Sobati H, Aliabadian M. Ecological modeling and distribution analysis of digger scorpions: Odontobuthus doriae, Odonthubutus bidentatus (Scorpiones: Buthidae) and Scorpio maurus (Scorpiones: Scorpionidae) in Iran using the maximum entropy method. Appl Entomol Zool. 2020;55(1):17-24.

  1. Keshavarz Alikhani H, Bidmeshkipour A, Zargan J. Cytotoxic and Apoptotic Induction Effects of the Venom of Iranian Scorpion (Odontobuthus bidentatus) in the Hepatocellular Carcinoma Cell Line (HepG2). Int J Pept Res. 2020;26(4):2475-84.
  2. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1):248-54.
  3. Kärber G. Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1931;162(4):480-3.
  4. Spearman C. The Method Of ‘Right And Wrong Cases’ (‘Constant Stimuli’) Without Gauss's Formulae. Br J Psychol. 1908;2(3):227-42.
  5. Shahbazi B, Najafabadi ZS, Goudarzi H, Sajadi M, Tahoori F, Bagheri M. Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells. J Venom Anim Toxins Incl Trop Dis. 2019;25:e20190009.
  6. Hassan F. Handbook of natural toxin. Production of scorpion antivenin. 2nd ed: Marcel Dekker; 1984. p. 577-605.
  7. Jalali A, Rahim F. Epidemiological review of scorpion envenomation in Iran. Iran J Pharm Res. 2014;13(3):743-56.
22.          Watt DD, Simard JM. Neurotoxic Proteins in Scorpion Venom. J Toxicol Toxin Rev. 1984;3(2-3):181-221.

  1. Karataş A, Karataş A. Mesobuthus eupeus (C.L. Koch, 1839) (Scorpiones: Buthidae) in Turkey. 2003;2003:1-6.
  1. Khosravi M, Mayahi M, Jalali SM, Rezaie A, Taghavi Moghadam A, Hosseini Z, et al. Effects of experimental Mesobuthus eupeus scorpion envenomation on chicken. Arch Razi Inst. 2017;72(1):23-31.
  2. Abdel-Rahman MA, Omran MA, Abdel-Nabi IM, Nassier OA, Schemerhorn BJ. Neurotoxic and cytotoxic effects of venom from different populations of the Egyptian Scorpio maurus palmatus. Toxicon. 2010;55(2-3):298-306.
  3. Omran MA. Cytotoxic and apoptotic effects of scorpion Leiurus quinquestriatus venom on 293T and C2C12 eukaryotic cell line. J Venom Anim Toxins Incl Trop Dis. 2003;9.
  4. Heinen TE, da Veiga AB. Arthropod venoms and cancer. Toxicon. 2011;57(4):497-511.
  5. Salem ML, Shoukry NM, Teleb WK, Abdel-Daim MM, Abdel-Rahman MA. In vitro and in vivo antitumor effects of the Egyptian scorpion Androctonus amoreuxi venom in an Ehrlich ascites tumor model. Springerplus. 2016;5:570.
  6. Al-Asmari AK, Riyasdeen A, Abbasmanthiri R, Arshaduddin M, Al-Harthi FA. Scorpion (Androctonus bicolor) venom exhibits cytotoxicity and induces cell cycle arrest and apoptosis in breast and colorectal cancer cell lines. Indian J Pharmacol. 2016;48(5):537-43.
30.          Gupta SD, Gomes A, Debnath A, Saha A, Gomes A. Apoptosis induction in human leukemic cells by a novel protein Bengalin, isolated from Indian black scorpion venom: through mitochondrial pathway and inhibition of heat shock proteins. Chem Biol Interact. 2010;183(2):293-303.

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