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محتوای ترکیب‌های فنلی و فعالیت آنزیم‌های PAL و PPO گیاه ریحان سبز (Ocimum basilicum L.) با پیش‌تیمار کروناتین تحت سمّیت آرسنیک (As) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 6، دوره 31، شماره 1 - شماره پیاپی 69، فروردین 1394، صفحه 52-62 اصل مقاله (414.35 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2015.12610 | ||
| نویسندگان | ||
| سعید زارع ده‌آبادی* 1؛ زهرا اسرار2؛ عبدالحمید نمکی شوشتری3؛ میترا مهربانی4 | ||
| 1دانشجوی دکتری، گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید باهنر کرمان | ||
| 2دانشیار، گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید باهنر کرمان | ||
| 3استادیار، گروه زیست‌شناسی، دانشکده علوم، دانشگاه شهید باهنر کرمان | ||
| 4دانشیار، گروه فارماکوگنوزی، دانشکده داروسازی، دانشگاه علوم پزشکی کرمان | ||
| چکیده | ||
| افزایش ذخیره آنتی‌اکسیدانی به‌خصوص تجمع محتوای ترکیب‌های فنلی یکی از مهمترین راهکارهای غیرآنزیمی گیاهان برای مقابله در برابر تنش‌های محیطی می‌باشد. در این پژوهش نمونه‌های ریحان سبز (Ocimum basilicum L.) در شرایط گلخانه‌ای تحت تیمار غلظت‌های مختلف کروناتین (0، 50 و 100 نانومولار) به‌عنوان پیش‌تیمار و فلز سنگین آرسنیک (0، 150 و 300 میکرومولار) رشد داده شدند. سپس شاخص‌های مورد نظر در نمونه‌های تیمار شده اندازه‌گیری شد. افزایش میزان پراکسیداسیون لیپیدها و نشت یونی غشاء و تجمع بالای پراکسید هیدروژن در برگ‌های ریحان از علائم سمّیت آرسنات بود که در اثر استفاده از پیش‌تیمار کروناتین به‌طور معنی‌داری از مقدار آنها کاسته شد. تجمع ترکیب‌های فنلی، آنتوسیانین‌ها، فلاونوییدها و همچنین افزایش فعالیت آنزیم‌های فنیل‌آلانین آمونیالیاز (PAL) و پلی‌فنل اکسیداز (PPO) توسط کروناتین در نمونه‌های تحت سمّیت آرسنیک نشان می‌دهد که شاید کروناتین به‌عنوان یک ترکیب مشابه با جاسمونات‌ها از طریق افزایش ذخیره آنتی‌اکسیدانی گیاه ریحان مقاومت آن را در برابر سمّیت فلز آرسنیک بهبود بخشیده است. | ||
| کلیدواژه‌ها | ||
| آرسنیک؛ کروناتین؛ ترکیب‌های فنلی؛ PAL؛ PPO | ||
| عنوان مقاله [English] | ||
| Phenolics content and PAL and PPO activity of Ocimum basilicum L. with coronatine pretreatment under arsenic toxicity | ||
| نویسندگان [English] | ||
| S. Zare Dehabadi1؛ Z. Asrar2؛ A. Shoushtari3؛ M. Mehrabani4 | ||
| 1Ph.D Student, Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman | ||
| 2Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 3Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 4Department of Pharmacognozy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran | ||
| چکیده [English] | ||
| Enhancing antioxidant pool especially phenolic compounds accumulation is one of the most important non enzymatic tools of plants to resist environmental stresses. In this investigation, basil (Ocimum basilicum L.) seedlings, grown in a control condition and irrigated with Hoagland nutrient solution, were treated withcoronatine (COR) at 0 (Control), 50 and 100 nM and different concentrations of As (V) at 0 (Control), 150 and 300 µM. Then some parameters in treated plants were measured. Increasing of lipid peroxidation and electrolyte leakage and H2O2 accumulation in basil leaves were the symptoms of arsenate toxicity that were ameliorated by COR pretreatment. Increasing phenolic compound contents, flavonoid and anthocyanin content and enhancing PAL and PPO activity indicate that COR as a jasmonates analogue may ameliorate arsenic toxicity in O. basilicum seedlings by improving antioxidative defense system. | ||
| کلیدواژه‌ها [English] | ||
| Arsenic, coronatine, Phenolic compounds, PAL, PPO | ||
| مراجع | ||
|
- Alexieva, V., Sergiev, I., Mapelli, S. and Karanov, E., 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant, Cell and Environment, 24(12): 1337-1344. - Ben Hamed, K., Castagna, A., Salem, E., Ranieri, A. and Abdelly, C., 2007. Sea fennel (Crithmum maritimum L.) under salinity conditions: a comparison of leaf and root antioxidant responses. Plant Growth Regulation, 53(3): 185-194. - Brooks, D.M., Bender, C.L. and Kunkel, B.N., 2005. The Pseudomonas syringae phytotoxin coronatine promotes virulence by overcoming salicylic acid-dependent defences in Arabidopsis thaliana. Molecular Plant Physiology, 6(6): 629-639. - Chu, Y.H., Chang, C.L. and Hsu, H.F., 2000. Flavonoid content of several vegetable and their antioxidant activity. Journal of the Science of Food and Agriculture, 80(5): 561-566. - Dixon, R.A. and Paiva, N.L., 1995. Stress-induced phenylpropanoide metabolism. The Plant Cell, 7(7): 1085-1097. - Feys, B.J.F., Benedetti, C.E., Penfold, C.N. and Turner, J.G., 1994. Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogen. The Plant Cell, 6(5): - Gao, X., Ohlander, M., Jeppsson, N., Bjork, L. and Trajkovski, V., 2000. Changes in antioxidant effects and their relationship to phytonutrients in fruit of sea buckthorn (Hippophae rhamnoides L.) during maturation. Journal of Agricultural and Food Chemistry, 48(5): 1485-1490. - Garg, N. and Singla, P., 2011. Arsenic toxicity in crop plants: physiological effects and tolerance mechanisms. Environmental Chemistry Letters, 9(3): 303-321. - Gupta, M., Sharma, P., Sarin, N.B. and Sinha, A.K., 2009. Differential response of arsenic stress in two varieties of brassica juncea L. Chemosphere, 74(9): 1201-1208. - Heath, R.L. and Pacher, L., 1968. Photo peroxidation in isolated chloroplast: I. kinetic and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 189-190. - Kar, M. and Mishra, D., 1976. Catalase, peroxidase, polyphenol oxidase activities during rice senescence. Plant Physiology, 57(2): 315-319. - Khan, A.G., Kuek, T.M., Chaudhury, T.M., Khoo, C.S. and Hayes, W.J., 2000. Role of plants, mycorrhizhae and phytochelators in heavy metal contaminated land remediation. Chemosphere, 41: 197-207. - Kim, H.J., Chen, F., Wang, X. and Rajapakse, N.C., 2006. Effect of methyl jasmonate on secondary metabolites of sweet basil (Ocimum basilicum L.). Journal of Agricultural Food Chemistry, 54: 2327-2332. - Kovacik, J. and Backor, M., 2007. Phenylalanine ammonia-lyase and phenolic compounds in chamomile tolerance to cadmium and copper excess. Water, Air, and Soil Pollution, 185: 185-193. - Krizek, D.T., Britz, S.J. and Mirecki, R.M., 1998. Inhibitory effect of ambient levels of solar UV-A and UV-B radiation on growth of cv. new red fire lettuce. Physiologia Plantarum, 103: 1-7. - Li, W.X., Chen, T.B., Huang, Z.C., Lei, M. and Liao, X.Y., 2006. Effect of arsenic on chloroplast ultrastructure and calcium distribution in arsenic hyperaccumulator Pteris vittata L. Chemosphere, 62(5): 803-809. - Li, Z., Wang, X., Chen, F. and Kim, H.J., 2007. Chemical changes and overexressed genes in sweet basil (Ocimum basilicum L.) upon methyl jasmonate treatment. Journal of Agricultural food chemistry, 55(3): 706-713. - Michalak, A., 2006. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies, 15(4): 523-530. - Mithofer, A., Maitrejean, M. and Boland, W., 2005. Structural and biological diversity of cyclic octadecanoids, jasmonates, and mimetics. Journal of Plant Growth Regulation, 23(3): 170-178. - Ozcan, M. and Chalchat, J. C., 2002. Essential oil composition of Ocimum basilicum L. and Ocimum minimum L. in Turkey. Czech Journal of Food Sciences, 20(6): 223-228. - Rice-Evans, C.A., Miller, N.J. and Paganaga, G., 1997. Antioxidant properties of phenolic compounds. Trends in Plant Science, 2(4): 152-159. - Saiedian, S., Keyhani, E. and Keyhani, J., 2007. Polyphenol oxidase activity in dormant saffron (Crocus sativus L.) corn. Acta Physiologia Plantarum, 29: 463-471. - Sakihama, Y., Cohen, M.F., Grace, S.C. and Yamasaki, H., 2002. Plant phenolics antioxidant and prooxidant activity: phenolics-induced oxidative damage mediated by metal in plants. Toxicology, 177: 67-80. - Shri, M., Kumar, S., Chakrabarty, D., Trivedi, P.K., Mallick, S., Misra, P., Shukla, D., Mishra, S., Srivastava, S., Tripathi, R.D. and Tuli, R., 2009. Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings. Ecotoxicology and Environmental Safety, 72(4): 1102-1110. - Singh, K., Kumar, S., Rani, A., Gulati, A. and Ahoja, P.S., 2009. Phenylalanine ammonia lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea. Functional and Integrative Genomics, 9(1): 125-134. - Solecka, D., 1997. Role of phenylpropanoid compounds in plant responses to different stress factor. Acta Physiologia Plantarum, 19(3): 257-268. - Tamogami, S. and Kodama, O., 2000. Coronatine elicits phytoalexin production in rice leaves (Oryza sativa L.) in the same manner as jasmonic acid. Phytochemistry, 54(7): 689-694. - Tanaka, Y., Kojima, M. and Uritani, I., 1974. Properties, development and cellular-localization of cinnamic acid 4-hydroxylase in cut injured sweet potato. Plant and Cell Physiology, 15(5): 843-854. - Tolbert, N.E., 1973. Activation of polyphenol oxidase of chloroplasts. Plant Physiology, 51(2): 234-244. - Trebst, A. and Depka, B., 1995. Polyphenol oxidase and photosynthesis research. Photosynthesis Research, 46: 41-44. - Turkan, I., Bor, M., Ozdemir, F. and Koca, H., 2005. Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolia Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science, 168: 223-231. - Vaughn, K.C. and Duke, S.O., 1984. Function of polyphenol oxidase in higher plants. Physiologia Plantarum, 60: 106-112. - Wang, B., Li, Z., Eneji, E.A., Tian, X., Zhai, Z., Li, J. and Duan, L., 2008. Effects of coronatine on growth, gas exchange traits, chlorophyll content, antioxidant enzymes and lipid peroxidation in maize (Zea mays L.) seedling under simulated drought stress. Plant Production Science, 11(3): 283-290. - Wang, L., Chen, W.J., Wang, Q., Eneji, A.E., Li, Z.H. and Duan, L.S., 2009. Coronatine Enhances Chilling tolerance in Cucumber (Cucumis sativus L.) seedlings by improving the antioxidative defence system. Journal of Agronomy and Crop Science, 195(5): 377-383. - Wanger, G.J., 1979. Content and vacuole/ extra vacuole distribution of neutral sugars, free amino acids and anthocyanins in protoplasts. Plant Physiology, 64: 88-93. - Wen, P.F., Chen, J.Y., Wan, S.B., Kong, W.F., Zhang, P., Wang, W., Zhan, J., Pan, Q.H. and Hung, W.D., 2008. Salicylic acid activates phenylalanine ammonia-lyase in grape berry in response to high temperature stress. Plant Growth Regulation, 55(1): 1-10. - Xie, Z.X., Duan, L.S., Tian, X.L., Wang, B.Q., Eneji, A.E. and Li, Z.H., 2008. Coronatine alleviates salinity stress in cotton by improving the antioxidative defense system and radical-scavenging activity. Journal of Plant Physiology, 165(4): - Xu, M.J. and Dong, J.F., 2005. Elicitor-induced nitric oxide burst is essential for triggering catharanthine synthesis in Catharanthus roseus suspension cells. Applied Microbiology and Biotechnology, 67: | ||
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