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تنوع آللی مکان‌های ژنی مربوط به بهاره‌سازی و ارتباط آن با عادت رشد در مزرعه لاین های امیدبخش گندم نان ( .Triticum aestivum L) | ||
| نهال و بذر | ||
| دوره 37، شماره 3، مهر 1400، صفحه 267-292 اصل مقاله (1.49 M) | ||
| شناسه دیجیتال (DOI): 10.22092/sppi.2022.126276 | ||
| نویسندگان | ||
| علی قربانی1؛ حبیب اله قزوینی* 2؛ رقیه امینیان3؛ سید شهریار جاسمی2؛ اشکبوس امینی2 | ||
| 1گروه اصلاح نباتات، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران. | ||
| 2موسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران. | ||
| 3گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی و منابع طبیعی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران. | ||
| چکیده | ||
| گندم نان مهم‌ترین گیاه زراعی است که در سراسر جهان کشت می‌شود و سازگاری گسترده‌ای با شرایط اقلیمی مناطق مختلف دارد. یکی از عوامل مهم این سازگاری نقش ژن‌های بهاره‌سازی در ژنوتیپ‌های مختلف گندم نان می‌باشد و شناسایی تنوع آللی این ژن‌ها امکان تعیین عادت رشد، پیش‌بینی سطح تحمل سرما و سازگاری ژنوتیپ‌ها در اقلیم‌های مختلف را فراهم می‌سازد. در این پژوهش، ارزیابی عادت رشدی 40 ژنوتیپ امیدبخش گندم نان در طی دو سال زراعی در مزرعه و یک سال در گلخانه انجام شد و همچنین تنوع آللی موجود در مکان‌های ژنی VRN-1 و VRN-3 با استفاده از نه جفت آغازگر اختصاصی تعیین گردید. در مکان ژنی VRN-A1، آلل‌هایVrn-A1a ، Vrn-A1b، Vrn-A1c وvrn-A1 بترتیب دارای فراوانی‌های 5/7%، 5/7% ، 10% و 5/67% بودند. در این مکان ژنی سه ژنوتیپ فاقد باند بودند. در مکان ژنی VRN-B1، آلل‌های غالب و مغلوب بترتیب دارای فراوانی‌های 5/52% و 5/47% و در مکان ژنی VRN-D1، این آلل‌ها به ترتیب دارای فراوانی‌های 60% و40% بودند. در مکان ژنی VRN-3 اکثریت ژنوتیپ‌ها (5/97%) دارای آلل vrn-B3 بودند. نتایج این پژوهش نشان داد که آلل‌های مختلف مکان ژنی VRN-A1 نسبت به دو مکان ژنی VRN-B1 و VRN-D1 نقش مهم‌تری در ایجاد تنوع عادت رشدی داشتند و ترکیب‌های مختلف این آلل‌ها منجر به ایجاد طیف متنوعی از کلاس‌های رشدی از عادت رشدی بهاره کامل تا زمستانه کامل شد. نتایج این پژوهش می‌تواند در تجمیع آلل‌های مطلوب ژن‌های بهاره‌سازی برای تولید ژنوتیپ‌های گندم سازگار با شرایط اقلیمی مناطق مختلف کشور در برنامه های به نژادی گندم نان مورد استفاده قرار گیرد. | ||
| کلیدواژه‌ها | ||
| گندم نان؛ آلل غالب؛ آلل مغلوب؛ ژن‌های بهاره‌سازی و آغازگر اختصاصی | ||
| عنوان مقاله [English] | ||
| Allelic Variation in Vernalization Loci and Its Relationship with Field-Based Growth Habit of Bread Wheat (Triticum aestivum L.) Promising Lines | ||
| نویسندگان [English] | ||
| A. Ghorbani1؛ H. Ghazvini2؛ R. Aminian3؛ Sh. Jasemi2؛ A. Amini2 | ||
| 1Plant Breeding Group, Faculty of Agriculture and Natural Resources Sciences, Imam Khomeini International University, Ghazvin, Iran. | ||
| 2Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran. | ||
| 3Plant Breeding Group, Faculty of Agriculture and Natural Resources Sciences, Imam Khomeini International University, Ghazvin, Iran. | ||
| چکیده [English] | ||
| Bread wheat is one of the most important food crops that is grown in different climatic conditions across the world. One of the important factors for this wide adaptability is the role of vernalization genes in different wheat genotypes. Therefore, identification of allelic variations of these genes provides an opportunity to determine growth habit, prediction of cold tolerance and adaptability of bread wheat genotypes to different climatic conditions. In this study, growth habit of 40 promising bread wheat genotypes was evaluated in the field and glasshouse during two and one cropping seasons, respectively. Allelic variation in VRN-1 and VRN-3 loci was also determined using nine locus-specific primer pairs. In VRN-A1 locus; Vrn-A1a, Vrn-A1b, Vrn-A1c and vrn-A1 alleles had frequencies of 7.5%, 7.5%, 10% and 67.5%, respectively. In this locus, three genotypes did not produce any band. In VRN-B1 locus; dominant and recessive alleles had frequencies of 52.5% and 47.5%, respectively, and in VRN-D1 locus; dominant and recessive alleles had frequencies of 60%, 40%, respectively. In VRN-3 locus; the majority of genotypes (97.5%) had recessive allele (vrn-B3). Results of this study indicated that different alleles of VRN-A1 locus had more influence on field growth habit as compared to VRN-B1 and VRN-D1 loci, and different combinations of these alleles resulted in range of various field growth habits from true spring to true winter. The results of this study can be used in the national bread wheat breeding programs for pyramiding favorite alleles of vernalization genes to improve adaptability of bread wheat genotypes for different target agro-climatic zones of Iran. | ||
| کلیدواژه‌ها [English] | ||
| Bread wheat, dominant allele, recessive allele, vernalization genes and allele-specific primer | ||
| مراجع | ||
|
Ahmadi, K., Ebadzadeh, H. R., Hatami, F., Abdolshah, H., and Kazemian, A. 2020. Agricultural statistics: 2018-19. Cropping cycle. 1st volume. Field crops. Information and Communication Technology Center. Deputy of Planning and Economy. Ministry of Jihad-e-Agriculture. 97 pp. (in Persian).
Ahmadi, K., Ebadzadeh, H. R., Hatami, F., Mohamadnia Afroozi, Sh., Esfandiari Pour, E., and Abass Taleghani., R. 2021. Agricultural statistics: 2019-20 Cropping cycle. 1st volume. Field crops. Information and Communication Technology Center. Deputy of Planning and Economy. Ministry of Jihad-e-Agriculture. 97 pp. (in Persian).
Anonymous. 2022. Agricultural insurance fund. Agriculture bank of Iran. Available on: http://www.sbkiran.ir/news/2578. (in Persian).
Chen, F., Gao, M., Zhang, J., Zuo, A., Shang, X., and Cui, D. 2013. Molecular characterization of vernalization and response genes in bread wheat from the yellow and Huai valley of China. BMC Plant Biology 13 (1): 199 DOI: https://doi.org/10.1186/1471-2229-13-199.
Danyluk, J., Kane, N. A., Breton, G., Limin, A. E., Fowler, D. B., and Sarhan, F. 2003. Ta VRT-1, a putative transcription factor associated with vegetative to reproductive transition in cereals. Plant Physiology 132 (4): 1849-1860.
Derakhshan, B., Mohammadi, S. A., Moghaddam, M., and Jalal Kamali, M. R. 2013. Molecular characterization of vernalization genes in Iranian wheat landraces. Crop Breeding Journal 3 (1): 1-11.
Dennis, E. S., and Peacock, W. J. 2009. Vernalization in cereals. Journal of Biology 8 (6): 57. https://doi.org/10.1186/jbiol156.
Distelfeld, A., Li, C., and Dubcovsky, J. 2009. Regulation of flowering in temperate cereals. Plant Biology 12: 178-184.
Dyck, J. A., Matus-cadiz, M. A., Hucl, P., Talbert, L., Hunt, T., Dubuc, J. P., Nass, H., Clayton, G., Dobb, J., and Quick, J. 2004. Agronomic performance of hard red spring wheat isolines sensitive and insensitive to photoperiod. Crop Science 44: 1976-1981.
Enghiad, A., Ufer, D., Countryman, A. M., and Thilmany, D. D. 2017. An overview of global wheat market fundamentals in an era of climate concerns. International Journal of Agronomy: 1-15. https://doi.org/10.1155/2017/3931897.
FAO, 2020. FAOStatistics. 2020. Food and Agrculture Organization of the United Nations. Rome, Italy. Available at: https://www.fao.org/faostat/en/.
Fu, D., Szűcs, P., Yan, L., Helguera, M., Skinner, J. S., Von Zitzewitz, J., Hayes, P. M., and Dubcovsky, J. 2005. Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat. Molecular Genetics and Genomics 273 (1): 54-65.
Hemming, M. N., Fieg, S., Peacock, W. J., Dennis, E. S., and Trevaskis, D. 2009. Regions associated with repression of the barley (Hordeum vulgare) vernalization1 gene are not required for cold induction. Molecular Genetics and Genomics 282: 107-117.
Iqbal, M., Shahzad, A., and Ahmed, I. 2011. Allelic variation at the Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3 and Ppd-D1a loci of Pakistani spring wheat cultivars. Electronic Journal of Biotechnology 14 (1): 1-8.
Kiss, T., Balla, K., Veisz, O., Lang, L., Bedo, Z., Griffiths, S., Isaac, P., and Karsai, I. 2014. Allele frequencies in the VRN-A1, VRN-B1 and VRN-D1 vernalization response and PPD-B1 and PPD-D1 photoperiod sensitivity genes, and their effects on heading in a diverse set of wheat cultivars (Triticum aestivum L.). Molecular Breeding 34: 297-310.
Lozada, D. N., Carter, A. H., Esten, R., and Mason, R. 2021. Unlocking the yield potential of wheat: influence of major growth habit and adaptation genes. Crop Breeding, Genetics and Genomics 3 (2): 1-28.
McIntosh, R. A., Dubcovsky, J., Rogers, W. J., Xia, X. C., and Raupp, W. J. 2020. Catalogue of gene symbols for wheat (supplement). Annual Wheat Newsletter 66: 1-20.
Mohammadfam, M., Gholkari, S., and Sadeghzade, B., 2016. Molecular characterization of allelic variation at the VRN-1 and VRN-B3 loci controlling vernalization requirement in wheat genotypes based on polymorphism in the promoter and intron regions. Iranian Journal of Dryland Agriculture 5 (2): 185-201 (in Persian).
Mohammadi, M., Torkamaneh, D., and Mehrazar, E. 2012. Molecular examination and genotype diversity of vernalization sensitivity and photoperiod response in old and modern bread wheat cultivars grown in Iran. Journal of Crop Science and Biotechnology 15 (4): 259-265.
Nazari, M., Zeinalinezhad, Kh., Navabpour, C., Soltanloo, H., and Pahlavani, M. H. 2018. Use of specific allele markers in identifying VRN-1 gene loci alleles in bread wheat genotypes. Journal of Crop Breeding 32: 133-143 (in Persian).
Nowak, M., and Kowalczyk, K. 2010. Allelic variation at the VRN-1 locus of polish cultivars common wheat (Triticum aestivum L.). Acta Biologica Cracoviensia Series Botanica 52 (2): 86-91.
Pugsley, A. T. 1971. A genetic analysis of the spring-winter habit of growth in wheat. Crop and Pasture Science 22 (1): 21-31.
Sadeghian Motahar, Y., Mozafari, J., Jalal Kamali, M. R., Dehghani Shoraki, Y., Ahmadi, M. R., Hassani, D., Shafaadin, S., Khaksar, K., Jazaieri, M. R., Najafian, G., and Esmaeilzadeh Moghaddam, M. 2007. National guidelines for tests of distinctness, uniformity and stability in bread wheat. Seed and Plant Certification and Registration Institute. 53 pp. (in Persian).
Saghai-Maroof, M. A., Soliman, K. M., Jorgensen R, A., and Allard, R. W. 1984. Ribosomal DNA spacer -length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proceedings of the National Academy of Sciences of the United States of America 81: 8014-8019.
Saibo, N. J., Lourenco, T., and Oliveira, M .2009. Transcription factors and regulation of photosynthetic and related metabolism under environmental stresses. Annals of Botany 103 (4): 609-623.
Snape, J. W., Butterworth, K., Whitechurch, E., and Worland, A. J. 2001. Waiting for fine times: genetics of flowering time in wheat. Euphytica 119 (1-2): 185-190.
Stelmakh, A. F. 1993. Genetic effects of Vrn genes on heading date and agronomic traits in bread wheat. Euphytica 65: 53-60.
Sung, S., and Amasino, R. M. 2004. Vernalization and epigenetics: how plants remember winter. Current Opinion in Plant Biology 7 (1): 4-10.
Tranquilli, G., and Dubcovsky, J. 2000. Epistatic interaction between vernalization genes Vrn-Am1 and Vrn-Am2 in diploid wheat. Journal of Heridity 91 (4): 304-306.
Trevaskis, B., Hemming, M. N., Dennis, E. S., and Peacock, W. J. 2007. The molecular basis of vernalization-induced flowering in cereals. Trends in Plant Science 12 (8): 352-357.
Trevaskis, B. 2010. The central role of the VERNALIZATION1 gene in the vernalization response of cereals. Functional Plant Biology 37 (6): 479-487.
Worland, A. J., and Snape, J. W. 2001. Genetic basis of worldwide wheat varietal improvement. In: pp. 61-67. Bonjean, A. P. Angus, W. J. (eds.). The world wheat book: A history of wheat breeding. Lavoisier Publishing, Paris, France.
Yan, L., Loukoianov, A., Tranquilli, G., Helguera, M., Fahima, T., and Dubcovsky, J. 2003. Positional cloning of the wheat vernalization gene VRN1. Proceedings of the National Academy of Science of America 100 (10): 6263-6268.
Yan, L., Loukoianov, A., Blechl, A., Tranquilli, G., Ramakrishna, W., Sanmiguel, P., Bennetzen J. L., Echenique V., and Dubcovsky, J. 2004a. The wheat VRN2 gene is a flowering repressor down-regulated by vernalization. Science 303 (5664): 1640-1644.
Yan, L., Helguera, M., Kato, K., Fukuyama, S., Sherman, J., and Dubcovsky, J. 2004b. Allelic variation at the VRN-1 promoter region in polyploid wheat. Theoretical and Applied Genetics 109 (8): 1677-1686.
Yan, L., Fu, D., Li, C., Blechl, A., Tranquilli, G., Bonafede, M., Sanchez, A., Valarik, M., Yasuda, S., and Dubcovsky, J. 2006. The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proceedings of the National Academy of Sciences of America 103 (51): 19581-19586.
Zaitseva, O. I., and Lemesh, V. A. 2015. Allelic composition in the Vrn-A1, Vrn-B1, and Vrn-B3 genes of double haploid lines of hexaploid triticale. Russian Journal of Genetics 51: 653-660.
Zhang, X. K., Xiao, Y. G., Zhang, Y., Xia, X. C., Dubcovsky, J., and He, Z. H. 2008. Allelic variation at the vernalization genes, Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3 and in Chinese wheat cultivars and their association with growth habit. Crop Science 48 (2): 458-470.
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