Investigation of TNFα level and Metallothionein gene expression in livers of rats exposed to dietary aluminum

Jasim Mohammed, Bushra; Aso Taher, Tara; N Abdallah, Zena

doi:10.22092/ari.2022.358201.2180

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	Investigation of TNFα level and Metallothionein gene expression in livers of rats exposed to dietary aluminum
Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 23 فروردین 1401
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358201.2180
نویسندگان
Bushra Jasim Mohammed ¹؛ Tara Aso Taher²؛ Zena N Abdallah¹
¹Institute of Genetic Engineering and Biotechnology
²College Of Sciences for Women / University of Baghdad / Iraq2
چکیده
Aluminum chloride is a chemical compound widely used in both the pharmaceutical and industrial sectors. The goal of this study was to see how aluminum chloride affected TNF levels and metallothionein gene expression in rat livers. Rats have been used as an experimental model in this study, with sixteen Wistar rats distributed into four groups (n=4). The treated groups were received aluminum chloride (Sigma/USA) at a dose of 25g/kg body weight via the stomach tube as follows: group 1: No treated rats as control group, group 2 were treated with aluminum chloride for a period of eight weeks, group 3 were treated with aluminum chloride for a period of twelve weeks, group 4 were treated with aluminum chloride for a period of sixteen weeks. TNF-α was measured in liver tissue using an enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry and real-time (RT) PCR were used to analyze metallothionein gene expression in rat liver. To estimate TNF levels, the results revealed that levels were considerably higher (P˂0.01) in all work groups than in the control group, and also in group (4) of the 16-week treatment period (401± 22.1 ng/ml). For the immunohistochemistry assay, a gradient intensity of staining for liver tissue was observed, ranging from zero staining in the control group to moderate, medium, and high staining in the working groups after 8, 12, and 16 weeks of aluminum chloride treatment, respectively. The greatest amount of methylothionine expression was seen in the livers of group 4 treated with aluminum chloride for 16 weeks (15.5-fold), with a significant difference (P˂0.01) from the other working groups. In both immunohistochemical and RT-PCR experiments, aluminum administration had a substantial influence on TNFα levels and metallothionein expression in rat livers.
کلیدواژه‌ها
TNF؛ immunohistochemistry؛ aluminum chloride؛ RT-PCR؛ metallothionein

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Investigation of TNFα level and Metallothionein gene expression in livers of rats exposed to dietary aluminum