اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها3,014
تعداد مقالات33,693
تعداد مشاهده مقاله44,883,052
تعداد دریافت فایل اصل مقاله42,957,398
Jasim Mohammed, B, Aso Taher, T, Abdallah, Z. N. (1401). Investigation of TNFα Level and Metallothionein Gene Expression in Livers of Rats Exposed to Dietary Aluminum. سامانه مدیریت نشریات علمی, 77(4), 1439-1446. doi: 10.22092/ari.2022.358201.2180
B Jasim Mohammed; T Aso Taher; Z. N Abdallah. "Investigation of TNFα Level and Metallothionein Gene Expression in Livers of Rats Exposed to Dietary Aluminum". سامانه مدیریت نشریات علمی, 77, 4, 1401, 1439-1446. doi: 10.22092/ari.2022.358201.2180
Jasim Mohammed, B, Aso Taher, T, Abdallah, Z. N. (1401). 'Investigation of TNFα Level and Metallothionein Gene Expression in Livers of Rats Exposed to Dietary Aluminum', سامانه مدیریت نشریات علمی, 77(4), pp. 1439-1446. doi: 10.22092/ari.2022.358201.2180
Jasim Mohammed, B, Aso Taher, T, Abdallah, Z. N. Investigation of TNFα Level and Metallothionein Gene Expression in Livers of Rats Exposed to Dietary Aluminum. سامانه مدیریت نشریات علمی, 1401; 77(4): 1439-1446. doi: 10.22092/ari.2022.358201.2180

Investigation of TNFα Level and Metallothionein Gene Expression in Livers of Rats Exposed to Dietary Aluminum

Archives of Razi Institute
مقاله 17، دوره 77، شماره 4، مهر و آبان 2022، صفحه 1439-1446    اصل مقاله (798.33 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358201.2180
نویسندگان
B Jasim Mohammed* 1؛ T Aso Taher2؛ Z. N Abdallah1
1Institute of Genetic Engineering and Biotechnology
2College of Sciences for Women, University of Baghdad, Baghdad, Iraq
چکیده
Aluminum chloride is a chemical compound widely used in both pharmaceutical and industrial sectors. The present study aimed to assess the effect of aluminum chloride on TNF levels and metallothionein gene expression in rat livers. A total of 16 Wistar rats were used as an experimental model and assigned to four groups (n=4). The treated groups received aluminum chloride (Sigma/USA) at a dose of 25g/kg body weight via a feeding tube as follows: group 1: Non-treated rats as the control group, group 2 were treated with aluminum chloride for 8 weeks, group 3 were treated with aluminum chloride for 12 weeks, and group 4 received aluminum chloride for 16  weeks. The TNF-α was measured in liver tissue using an enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry and real-time polymerase chain reaction (RT-PCR) were used to analyze metallothionein gene expression in rat liver. To estimate TNF levels, the results revealed that levels were considerably higher (P˂0.01) in all experimental groups, especially in group 4 which underwent treatment for  16 weeks (401±22.1 ng/ml), as compared to that in the control group. For the immunohistochemistry assay, a gradient intensity of staining for liver tissue was observed, ranging from zero staining in the control group to moderate, medium, and high staining in the experimental groups after 8, 12, and 16 weeks of aluminum chloride treatment, respectively. The greatest amount of methylothionine expression was observed in the livers of group 4 which received aluminum chloride for 16 weeks (15.5-fold), with a significant difference (P˂0.01) from the other experimental groups. In both immunohistochemical and RT-PCR experiments, aluminum administration had a substantial influence on TNFα levels and metallothionein expression in rat livers.
کلیدواژه‌ها
Aluminum chloride؛ Immunohistochemistry؛ Liver؛ Metallothionein؛ RT-PCR؛ TNF
سایر فایل های مرتبط با مقاله
مراجع
  1. Rastogi A, Zivcak M, Sytar O, Kalaji HM, He X, Mbarki S, et al. Impact of metal and metal oxide nanoparticles on plant: a critical review. Front Chem. 2017;5:78.
  2. Balasubramanyam A, Sailaja N, Mahboob M, Rahman M, Hussain SM, Grover P. In vivo genotoxicity assessment of aluminium oxide nanomaterials in rat peripheral blood cells using the comet assay and micronucleus test. Mutagenesis. 2009;24(3):245-51.
  3. Prabhakar P, Reddy UA, Singh S, Balasubramanyam A, Rahman M, Indu Kumari S, et al. Oxidative stress induced by aluminum oxide nanomaterials after acute oral treatment in Wistar rats. J Appl Toxicol. 2012;32(6):436-45.
  4. Zhang Q, Li M, Ji J, Gao F, Bai R, Chen C, et al. In vivo toxicity of nano-alumina on mice neurobehavioral profiles and the potential mechanisms. Int J Immunopathol Pharmacol. 2011;24(1 Suppl):23-9.
  5. Chen L, Yokel RA, Hennig B, Toborek M. Manufactured aluminum oxide nanoparticles decrease expression of tight junction proteins in brain vasculature. J Neuroimmune Pharmacol. 2008;3(4):286-95.
  6. Riihimäki V, Valkonen S, Engström B, Tossavainen A, Mutanen P, Aitio A. Behavior of aluminum in aluminum welders and manufacturers of aluminum sulfate—impact on biological monitoring. Scand J Work Environ Health. 2008:451-62.
  7. Bharathi VP, Govindaraju M, Palanisamy A, Sambamurti K, Rao K. Molecular toxicity of aluminium in relation to neurodegeneration. Indian J Med Res. 2008;128(4):545-56.
  8. Lemire J, Mailloux R, Puiseux‐Dao S, Appanna V. Aluminum‐induced defective mitochondrial metabolism perturbs cytoskeletal dynamics in human astrocytoma cells. J Neurosci Res. 2009;87(6):1474-83.
  9. Takahashi S. Positive and negative regulators of the metallothionein gene. Mol Med Rep. 2015;12(1):795-9.
  10. Chung RS, Howells C, Eaton ED, Shabala L, Zovo K, Palumaa P, et al. The native copper-and zinc-binding protein metallothionein blocks copper-mediated Aβ aggregation and toxicity in rat cortical neurons. PLoS One. 2010;5(8):12030.
  11. Nielsen AE, Bohr A, Penkowa M. The Balance between Life and Death of Cells: Roles of Metallothioneins. Biomark Insights. 2007;1:99-111.
  12. Penkowa M, Cáceres M, Borup R, Nielsen FC, Poulsen CB, Quintana A, et al. Novel roles for metallothionein‐I+ II (MT‐I+ II) in defense responses, neurogenesis, and tissue restoration after traumatic brain injury: Insights from global gene expression profiling in wild‐type and MT‐I+ II knockout mice. J Neurosci Res. 2006;84(7):1452-74.
  13. Miles A, Hawksworth G, Beattie J, Rodilla V. Induction, regulation, degradation, and biological significance of mammalian metallothioneins. Crit Rev Biochem Mol Biol. 2000;35(1):35-70.
  14. Ma H, Su L, Yue H, Yin X, Zhao J, Zhang S, et al. HMBOX1 interacts with MT2A to regulate autophagy and apoptosis in vascular endothelial cells. Sci Rep. 2015;5(1):1-14.
  15. Krześlak A, Forma E, Jóźwiak P, Szymczyk A, Smolarz B, Romanowicz-Makowska H, et al. Metallothionein 2A genetic polymorphisms and risk of ductal breast cancer. Clin Exp Med. 2014;14(1):107-13.
  16. Mohammed BJ. Effect of gasoline inhalation on tumor necrosis factor-alpha (TNF-alpha) expression in liver of rats. Biosci Res. 2017;14(3):566-73.
  17. Sanai T, Okuda S, Onoyama K, Motomura K, Osato S, Hori K, et al. Effect of different doses of aluminium hydroxide on renal deterioration and nutritional state in experimental chronic renal failure. Miner Electrolyte Metabol. 1991;17(3):160-5.
  18. Lynch MJ. Medical laboratory technology and clinical pathology. 1969.
  19. Jasim B, Mohammed BJ. Investigation on the effect of different concentrations of chlorine drinking water on mice livers. Biochem Cell Arch. 2018;18.
  20. Lillie RD. Histopathologic technic and practical histochemistry: The Blakiston; 1954.
  21. Mohammed B. Study the inhibitory effect of Thuja occidentalis against Pseudomonas aeruginosa isolated from surgical wounds in vitro and in vivo. Int J Sci Nat. 2017;8(2):352-7.
  22. Sambrook J, Russell DW. The condensed protocols from molecular cloning: a laboratory manual. 2006.
  23. Wang X, Seed B. A PCR primer bank for quantitative gene expression analysis. Nucleic Acids Res. 2003;31(24):154.
  24. Mohammed BJ, AL-Thwani AN, Kadhim AAA. Effect of Nicotine Injection on Tumor Suppressor Gene P53 Expression in Lung of Mice. Indian J Ecol. 2020;47(12):41-4.
  25. Zhang CC, Volkmann M, Tuma S, Stremmel W, Merle U. Metallothionein is elevated in liver and duodenum of Atp7b (−/−) mice. BioMetals. 2018;31(4):617-25.
  26. Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 2002;30(9):36.
  27. Cary N. Statistical analysis system, User's guide. Statistical. Version 9. SAS Inst Inc USA. 2012.
  28. Wang Z, Li S, Cao Y, Tian X, Zeng R, Liao D-F, et al. Oxidative stress and carbonyl lesions in ulcerative colitis and associated colorectal cancer. Oxidative Med Cell Longev. 2016;2016.
  29. Madureira PA, Waisman DM. Annexin A2: the importance of being redox sensitive. Int J Mol Sci. 2013;14(2):3568-94.
  30. Viarengo A, Ponzano E, Dondero F, Fabbri R. A simple spectrophotometric method for metallothionein evaluation in marine organisms: an application to Mediterranean and Antarctic molluscs. Mar Environ Res. 1997;44(1):69-84.
  31. Coyle P, Philcox J, Carey L, Rofe A. Metallothionein: the multipurpose protein. Cell Mol Life Sci. 2002;59(4):627-47.
  32. Qiao X, Ma Z-Y, Shao J, Bao W-G, Xu J-Y, Qiang Z-Y, et al. Biological evaluation of a cytotoxic 2-substituted benzimidazole copper (II) complex: DNA damage, antiproliferation and apoptotic induction activity in human cervical cancer cells. Biometals. 2014;27(1):155-72.
  33. Zhang H, Liu X, Warden CD, Huang Y, Loera S, Xue L, et al. Prognostic and therapeutic significance of ribonucleotide reductase small subunit M2 in estrogen-negative breast cancers. BMC Cancer. 2014;14(1):1-16.
  34. Schwabe RF, Brenner DA. Mechanisms of liver injury. I. TNF-α-induced liver injury: role of IKK, JNK, and ROS pathways. Am J Physiol Gastrointest Liver Physiol. 2006;290(4):583-9.
  35. Beggs KM, Fullerton AM, Miyakawa K, Ganey PE, Roth RA. Molecular mechanisms of hepatocellular apoptosis induced by trovafloxacin-tumor necrosis factor-alpha interaction. Toxicol Sci. 2014;137(1):91-101.
  36. Chakraborty JB, Oakley F, Walsh MJ. Mechanisms and biomarkers of apoptosis in liver disease and fibrosis. Int J Hepatol. 2012;2012.
  37. Raudenska M, Gumulec J, Podlaha O, Sztalmachova M, Babula P, Eckschlager T, et al. Metallothionein polymorphisms in pathological processes. Metallomics. 2014;6(1):55-68.
  1. Guicciardi ME, Malhi H, Mott JL, Gores GJ. Apoptosis and necrosis in the liver. Compr Physiol. 2013;3(2).
  1. Krizkova S, Kepinska M, Emri G, Rodrigo MAM, Tmejova K, Nerudova D, et al. Microarray analysis of metallothioneins in human diseases—A review. J Pharm Biomed Anal. 2016;117:464-73.
آمار
تعداد مشاهده مقاله: 1,702
تعداد دریافت فایل اصل مقاله: 1,397


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب