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Study of microbial infections and some immunological parameters among Covid-19 of ICU patients in Najaf Governorate, Iraq | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 23 فروردین 1401 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.358102.2151 | ||
| نویسنده | ||
Ruaa SH 
| ||
| 1- Kufa University / Science College /Laboratory Investigation | ||
| چکیده | ||
| The current study was designed to investigate some microbial infections and immunological parameters associated with Covid-19 patients presenting in the intensive care unit (ICU) at Al-Amal Specialized Hospital in AL-Najaf Governorate from February and March of 2021.The study included 50 patients who were divided into two groups: 20 patients aged ≤ 70 years and 30 patients aged ≥70 years. The method of microbial culture was adopted to isolate bacteria and yeasts by collecting sputum specimens and oral swabs from patients and cultivating them on diagnostic media and then confirming the diagnosis with Vitek. Whereas, serum samples were collected from patients’ blood for the purpose of diagnosing fungal infections, and then studying some immunological criteria, which included diagnosing infection with the Corona virus by measuring IgM and IgG by FAIDs and studying the concentration of cytokines (IL-6 and IF ) using ELISA method. The results showed that bacterial species Streptococcus pneumonia 5 (25%), Haemophilus Influenzae 7 (35%), Moraxella catarrhalis 3 (15%) were isolated from the first age group of patients (≤ 70). The recorded data showed that Streptococcus pneumonia 10 (33.3%), Streptococcus pyogenes 5 (16.6%), Streptococcus viridians 1 (3.3%), Haemophilus Influenzae 6 (20%), Mycobacterium tuberclusis 2 (6.6%), Pseudomonas aeruginosa 2 (6.6%) were the isolated and identified microorganisms in the second age group (≥ 70). The results revealed that the isolated yeast from the first age group was Candida albicans 5 (25%) and Candida galbrata 3 (10%) while in the second age group 1 (3.3%) Candida albicans was isolated. The study proved that 30% and 10% of patients in the first and second age groups had invasive pulmonary aspergillosis (IPA) co-infection by detecting Galactomanan (GM) in the blood serum (1.05 ±0.59, 1.25 ±0.38) respectively .The results of measuring some immunological parameters IgM and IgG indicated that (11.42±6.82, 14.84±9.21) and (0.47±6.82, 0.12±0.11) For the first and second age groups respectively .While the results of IL-6 were (146.12±46.35, 133.28±116.94) For the first and second age groups, respectively. The average of IFɤ (98.37±65.70, 110.69±47.60) for the first two age groups respectively. Elderly patients with severe COVID-19 are entered into the ICU | ||
| کلیدواژه‌ها | ||
| IFɤ؛ IL-6؛ ICU؛ IgG؛ IgM and IPA | ||
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آمار تعداد مشاهده مقاله: 10 |
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