Analysis the nucleotide sequences similarity and protein prediction of some resistance genes in Escherichia coli isolated from Iraqi urinary tract infections patients.

Kadhim Mohammed, Rana; Attallah Ibrahim, Ali

doi:10.22092/ari.2022.357528.2058

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	Analysis the nucleotide sequences similarity and protein prediction of some resistance genes in Escherichia coli isolated from Iraqi urinary tract infections patients.
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مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 01 اردیبهشت 1401
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357528.2058
نویسندگان
Rana Kadhim Mohammed ؛ Ali Attallah Ibrahim
Department of Biotechnology, Collage of Science, University of Baghdad, Baghdad, Iraq.
چکیده
Antibiotics resistance lead to dramatic increase in the morbidity and mortality due to the infection disease, even though estimates vary widely, the economic cost of antibacterial to antibiotics resistance are increasing every year. The current was designed to identify and comprehend antimicrobial resistance of Escherichia coli (E. coli). In fact this study focused on the recent deep-learning methods (sequencing), to investigate E. coli antibiotic resistance and their protein sequences.To evaluate antibiotic resistance the sequencing method could be considered as the method of choice. Identification of E. coli using either specific biochemical tests or Polymerase chain reaction (PCR) detection using the 16S rRNA gene was used in this study. The results of aadA1 gene sequences showed 10 nucleic acid substitutions throughout compared with reference NCBI database (MG385063), from the 10 nucleic acid substitutions 9 missense effects were observed. While the dfrA1 gene sequences showed 20 nucleic acid substitutions throughout compared with reference NCBI database (KY706080), from the 20 nucleic acid substitutions 8 missense effects were observed. Furthermore the sul1 gene sequences showed 20 nucleic acid substitutions throughout compared with reference NCBI database (CP069561), from the 20 nucleic acid substitutions 12 missense effects were observed. The cat1 gene sequences showed 14 nucleic acid substitutions throughout compared with reference NCBI database (NC017660), from the 14 nucleic acid substitutions 8 missense effects were observed. The precise point (Missense) mutation in the four genes aadA1, dfrA1, sul1 and cat1 in the expected sequence is interpreted to be the target site of a site-specific recombination mechanism that led to antibiotics resistance in E. coli isolates.
کلیدواژه‌ها
Escherichia coli؛ Resistance genes؛ Sequencing

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Analysis the nucleotide sequences similarity and protein prediction of some resistance genes in Escherichia coli isolated from Iraqi urinary tract infections patients.