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Volatile Oil Composition of Nigella sativa Cultivars Collected from Iran | ||
| Journal of Medicinal plants and By-product | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 19 اردیبهشت 1401 اصل مقاله (710.1 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2022.357895.1455 | ||
| نویسندگان | ||
Zohre Ghanavi1؛ Ali Akbar Velayati2؛ Poopak Farnia2؛ Saeed Mollaei3؛ Jalaledin Ghanavi  3
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| 1Institute of Standard and Industrial Research of Iran, Tehran, Iran | ||
| 2Mycobacteriology Research Centre (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| 3Phytochemical Laboratory, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, 53714-161, Tabriz, Iran | ||
| چکیده | ||
| Nigella sativa L., belonging to the family of Ranunculaceae, is grown for its seeds, which are used in food and medicinal industries. In this study, the volatile oil compositions of 16 cultivars of N. Sativa collected from different parts of Iran were evaluated, and then the chemotaxonomic study was done. The analysis of volatile oils was done using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), which identified 22 components. Major constituents of the collected cultivars were p-cymene (27.7-38.1%), thymoquinone (19.5-40.9%), thymohydroquinone (5.8-9.6%), α-thujene (5.1-7.2%), and trans-4-methoxythujane (3.4-4.8%). Then, Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were used to assess differences in volatile oil composition. PCA displayed that different groups of cultivars were characterized by high contents of specific compounds. The results of chemometric analysis displayed that PCA was approximately in agreement with HCA, and allowed classification of the studied cultivars into four groups including Group-A (Ns-4, Ns-5, and Ns-16); Group-B (Ns-1), Group-C (Ns-2, Ns-3, Ns-7, Ns-8, and Ns-12), and Group-D (Ns-6, Ns-9, Ns-10, Ns-11, Ns-13, Ns-14, and Ns-15). This is the first study on N. sativa in a vast geographical context in Iran to this extent, using different chemometric techniques. The results of this study can offer scientific data for germplasm of N. sativa in Iran and for breeding programs to improve developed cultivars. | ||
| کلیدواژه‌ها | ||
| Black cumin؛ Geographical location؛ PCA؛ Thymoquinone؛ Volatile oil | ||
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آمار تعداد مشاهده مقاله: 9 تعداد دریافت فایل اصل مقاله: 8 |
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