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آمار
| تعداد نشریات | 280 |
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| تعداد شماره‌ها | 2,348 |
| تعداد مقالات | 27,179 |
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Bacteriological and Molecular Detection of Klebsiella oxytoca and its Resistance to Antibiotics among Clinical Specimens from Kirkuk, Iraq | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 30 اردیبهشت 1401 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.357753.2095 | ||
| نویسندگان | ||
Sarah Ahmed Hasan  ؛ Media Mohammed Bakr
| ||
| Department of Biology, Faculty of Education of Pure Science. University of Kirkuk, Iraq. | ||
| چکیده | ||
| Klebsiella spp. are gram-negative bacteria which considered as serious public health problem causing urinary tract infections (UTI), bloodstream infections, pneumonia and soft tissue infections. This study was designed to investigate the prevalence of Klebsiella oxytoca among clinical samples and determined their resistance against various antimicrobial drugs with molecular identification of K. oxytoca by polymerase chain reaction (PCR) technique using specific sequence of pehX gene. A total of 250 clinical samples including throat, wound, and vaginal swabs were obtained. Participants in this study were from both sexes and in different ages. The samples were streaked on blood and MacConkey agar. Antibiotic sensitivity test was made by modified Kirby-Bauer disc diffusion technique. Molecular identification of K. oxytoca was performed for all isolates. Out of 250 clinical samples, Klebsiella oxytoca was reported in 32 (12.8%) cases. Highest prevalence was observed in 18(18%) cases of throat swabs, followed by wound swabs in 16 (16%) cases and vagina in 6 (6%) cases. Females were more affected 22 (14.5%) with Klebsiella oxytoca than males 10 (10.10%). Infected participants with 15-40 years age old were more affected with Klebsiella oxytoca 23 (12.73%) than patients with 41-65 years age old 9 (9.67% ). The highest resistance pattern of Klebsiella oxytoca was 100% against Augmentin, Ampicillin, Cephalothin ,Pipracillin and Refampin followed by Ceftazidime, Cefixime , Cefotaxime, Trimethoprim and Aztreonam, with 62.50% , 59.37% , 53.12% , 53.12% and 50% respectively. Highest sensitivity was observed against amikacin and imipenem (9.37%) followed by Meropenem Chloramphenicol Nalidixic acid Ciprofloxacin Tobramycin Gentamycin and Doxycycline with 21.87%, 21.87%, 25%, 25%, 28.12%, 28.12%, and 28.12% respectively. Molecular identification of K. oxytoca was revealed that all isolates showed PCR product with 344 bp specific primer (pehX), that performed K. oxytoca. | ||
| کلیدواژه‌ها | ||
| Klebsiella oxytoca؛ PCR؛ antibiotic resistance؛ pehX gene؛ clinical samples | ||
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آمار تعداد مشاهده مقاله: 35 |
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