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Ali Mandeel, H, Shahid Jassim, A, Kadhum Naeem, A. (1401). Prevalence of Enterohaemorrhagic and Enteroaggregative E. coli among Children with Diarrhea in Najaf, Iraq. سامانه مدیریت نشریات علمی, 77(5), 1655-1658. doi: 10.22092/ari.2022.357755.2097
H Ali Mandeel; A Shahid Jassim; A Kadhum Naeem. "Prevalence of Enterohaemorrhagic and Enteroaggregative E. coli among Children with Diarrhea in Najaf, Iraq". سامانه مدیریت نشریات علمی, 77, 5, 1401, 1655-1658. doi: 10.22092/ari.2022.357755.2097
Ali Mandeel, H, Shahid Jassim, A, Kadhum Naeem, A. (1401). 'Prevalence of Enterohaemorrhagic and Enteroaggregative E. coli among Children with Diarrhea in Najaf, Iraq', سامانه مدیریت نشریات علمی, 77(5), pp. 1655-1658. doi: 10.22092/ari.2022.357755.2097
Ali Mandeel, H, Shahid Jassim, A, Kadhum Naeem, A. Prevalence of Enterohaemorrhagic and Enteroaggregative E. coli among Children with Diarrhea in Najaf, Iraq. سامانه مدیریت نشریات علمی, 1401; 77(5): 1655-1658. doi: 10.22092/ari.2022.357755.2097

Prevalence of Enterohaemorrhagic and Enteroaggregative E. coli among Children with Diarrhea in Najaf, Iraq

Archives of Razi Institute
مقاله 18، دوره 77، شماره 5، آذر و دی 2022، صفحه 1655-1658    اصل مقاله (367.45 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357755.2097
نویسندگان
H Ali Mandeel1؛ A Shahid Jassim2؛ A Kadhum Naeem* 3
1Ministry of Education, General Directorate of Education in Al-Qadisiyah, Al-Qadisiyah, Iraq
2Ministry of Education, General Directorate of Education in Najaf, Najaf, Iraq
3Department of Biology, College of Education for Girls, University of Kufa, Kufa, Iraq
چکیده
Diarrhea is one of the leading causes of morbidity and mortality among children under 5 years old in developing countries. The classification of diarrheagenic E. coli (DEC) strains among children with diarrhea is still receiving low attention. The present study aimed at determining the prevalence of enterohaemorrhagic E. coli (EHEC) and enteroaggregative E. coli (EAEC) among children under 5 years old suffering from acute diarrhea. Stool samples (n=100) were collected from children under 5 years old suffering from acute diarrhea for the molecular detection of EHEC (using stx1 and stx2) and EAEC (using aat) by polymerase chain reaction technique. The results showed a high percentage of isolation of EHEC from stool samples, in compression to EAEC; accordingly, among 75 identified DEC isolates, 15.9% belonged to EHEC, while 5.3% belonged to EAEC. Among EHEC, stx1 was highly prevalent among isolates (9.3%), in comparison to stx2 (6.6%). A high frequency of EHEC was detected in males in the age group of 7-12 months, whereas EAEC was found in females in the age group of 13-19 months. In conclusion, EHEC and EAEC were associated with bloody and watery diarrhea among children under 5 years old. Genes associated with virulence factors (i.e., stx1, stx2, and aat) could be used as genetic markers for the detection of EHAE and EAEC.
کلیدواژه‌ها
aat؛ Diarrhea؛ EAEC؛ EHEC؛ PCR؛ stx
مراجع
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