Synthesis of bio-active silver nanoparticles against human lung cancer cell line (A549) with little toxicity to normal cell line (WRL68)

M. Talib, Khudhair; G. Oraibi, Asma; I Abass, Gudeai

doi:10.22092/ari.2022.357979.2125

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	Synthesis of bio-active silver nanoparticles against human lung cancer cell line (A549) with little toxicity to normal cell line (WRL68)
Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 31 اردیبهشت 1401
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357979.2125
نویسندگان
Khudhair M. Talib ¹؛ Asma G. Oraibi²؛ Gudeai I Abass³
¹1The Iraqi Ministry of Education, Karkh II Education Directorate, Baghdad, Iraq.
²Al-Nahrain University, College of Biotechnology, Baghdad, Iraq.
³Iraqi University, College of Education, Baghdad, Iraq.
چکیده
Nanomaterials are characterized by mechanical, thermal, chemical, biological and other properties different from the basic materials that make them up due to their large surface area to size ratio and quantum effect. There are multiple ways to produce nanomaterials mechanically, chemically and physically, but they are not safe for the environment. Researchers have sought to find safe methods for the production of nanomaterials, such as green manufacturing, that is, manufacturing nanomaterials from plants, and there are other sources such as bacteria or fungi that are used in the production of nanomaterials. The aim of this study was to try to find an alternative to chemically manufactured drugs, such as those used in the treatment of human cancers, through nanotechnology manufactured from plant sources (green-biosynthesis), which is characterized by abundance and low economic cost. Silver nanoparticles were green-synthesized using aqueous extract of licorice plant, their properties were diagnosed and the differences were compared with the crude aqueous extract. The nanoparticles size ranged between 60.27 - 89.80 nm, while the sizes of the crude aqueous extract particles ranged between 53.96 - 113.1 nm. Atomic force microscopy was used to find out the shapes, topography, roughness and protrusions of the surfaces of biosynthesized AgNPs and aqueous extract particles, where the roughness rate of the nanoparticles was 75.54 nm, while it appeared. In vitro, AgNPs showed a higher anti-lung cancer activity against A549 cell line than that of the extract at an inhibitory concentration for half of the cells used in the experiment (IC50) 58.78 µg/ml, and the IC50 of the extract was 67.44 µg/ml, as well as the results showed that the toxicity of AgNPs on the normal hepatocyte line (WRL68) were less than the toxicity of the aqueous extract, with IC50 concentrations 244.2 and 147.0 µg/ml respectively, and it is worth mentioning that the lower the inhibitory concentration for half of the cells used in the experiment (IC50) was with higher toxicity.
کلیدواژه‌ها
Silver nanoparticles؛ Glycyrrhiza glabra L؛ licorice plant AgNPs؛ A549 cell line or WRL68 cell line

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Synthesis of bio-active silver nanoparticles against human lung cancer cell line (A549) with little toxicity to normal cell line (WRL68)