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| تعداد نشریات | 280 |
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Molecular Detection of Some Virulence Genes in Salmonella spp. Isolated from Clinical Samples in Iraq | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 01 خرداد 1401 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2022.357983.2129 | ||
| نویسنده | ||
Hanaa Farhan Abbas 
| ||
| Mustansiriyah university, College of sciences , Department of biology/Iraq | ||
| چکیده | ||
| Salmonella spp. are considerable source of diarrheal diseases everywhere and considered as one of the greatest dangerous foodborne bacteria. The objectives of the current study was to detect the occurrence of the most important virulence genes in Salmonella enterica among bacteria which isolated from stool in Baghdad's hospitals, Iraq. A total of 50 swab stool samples were collected from patients suffered from food poisoning attended to different hospitals in Baghdad city. The isolates were identified using morphological tests and confirmed by Vitek-2 system. To extract DNA from the isolates, a genomic DNA kit was used. Molecular detection of five virulence genes: invA, papC, spvC, stn, and fimH were performed by using PCR. Out of 50 swab samples, 40% (20 samples) of them confirmable as Salmonella enterica. Moreover, the prevalence of virulence genes by PCR demonstrated that all 20 S. enterica isolates carried at least one gene from the genes that associated to biofilm formation. The invA, stn and fimH were the most predominant genes, due to being existent in (100%) of the all 20 S. enterica isolates. The prevalence of the virulence genes papC and spvC was 75% (15/20), and 65% (13/20), respectively. The current data support the occurrence of Salmonella spp. exhibiting a broad range of virulence genes in stool samples from patients who had food poisoning which indeed make these bacteria provide such a significant threat to public health. | ||
| کلیدواژه‌ها | ||
| Salmonella؛ PCR؛ virulence genes؛ food-borne illnesses | ||
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آمار تعداد مشاهده مقاله: 112 |
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