اخبار و اعلانات

 آمار

تعداد نشریات283
تعداد نشریات سازمان81
تعداد شماره‌ها2,722
تعداد مقالات31,007
تعداد مشاهده مقاله27,037,856
تعداد دریافت فایل اصل مقاله16,153,902
Almansorri, A. K, Al-Shirifi, H. M. H, Al-Musawi, S, Ahmed, B. B. (1401). Investigation of the Inhibition Activity of Aluminium Oxide Nanoparticles for Herpes Simplex Type 1. سامانه مدیریت نشریات علمی, 78(1), 213-219. doi: 10.22092/ari.2022.358497.2237
A. K Almansorri; H. M. H Al-Shirifi; S Al-Musawi; B. B Ahmed. "Investigation of the Inhibition Activity of Aluminium Oxide Nanoparticles for Herpes Simplex Type 1". سامانه مدیریت نشریات علمی, 78, 1, 1401, 213-219. doi: 10.22092/ari.2022.358497.2237
Almansorri, A. K, Al-Shirifi, H. M. H, Al-Musawi, S, Ahmed, B. B. (1401). 'Investigation of the Inhibition Activity of Aluminium Oxide Nanoparticles for Herpes Simplex Type 1', سامانه مدیریت نشریات علمی, 78(1), pp. 213-219. doi: 10.22092/ari.2022.358497.2237
Almansorri, A. K, Al-Shirifi, H. M. H, Al-Musawi, S, Ahmed, B. B. Investigation of the Inhibition Activity of Aluminium Oxide Nanoparticles for Herpes Simplex Type 1. سامانه مدیریت نشریات علمی, 1401; 78(1): 213-219. doi: 10.22092/ari.2022.358497.2237

Investigation of the Inhibition Activity of Aluminium Oxide Nanoparticles for Herpes Simplex Type 1

Archives of Razi Institute
مقاله 28، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 213-219    اصل مقاله (604.49 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358497.2237
نویسندگان
A. K Almansorri* 1؛ H. M. H Al-Shirifi2؛ S Al-Musawi3؛ B. B Ahmed1
1Faculty of Biotechnology, Al-Qasim Green University, Babylon, Iraq
2Environmental Health Department, Environmental Sciences College, Al-Qasim Green University, Babylon Province, Iraq
3College of Food Sciences, Al-Qasim Green University, Babylon Province, Iraq
چکیده
Several studies have shown that Herpes simplex type 1 )HSV-1 (is one of the viruses resistant to medications, so potential antiherpetic agents need to be evaluated. This study aimed to evaluate the impact of Aluminum Oxide Nanoparticles (Al2O3-NPs) on HSV-1 infection. Characterization of Al2O3-NPs was performed using field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), dynamic light scattering (DLS), and high-resolution transmission electron microscopy (HRTEM). The MTT test was used to investigate the toxicity action of Al2O3-NPs on viable cells. Quantitative Real-Time PCR (qRT-PCR)and TCID50 assays were used to achieve the antiherpetic performance Al2O3-NPs.Indirect immunofluorescence assay (IFA) was performed to determine the inhibitory impact of Al2O3-NPs on viral antigen expression, and acyclovir was utilized as a standard agent in all tests. HSV-1 subjected to Al2O3-NPs at the maximum non-toxic concentration (100 μg / mL) leads to a decrease of 0.1, 0.7, 1.8, and 2.5 log10 TCID50 in the infectious titer relative to virus control (P<0.0001). This concentration of Al2O3-NPs was correlated with 16.9%, 47.1 %, 61.2 %, 72.5 % and 74.6 % inhibition rates, calculated based on HSV-1 viral load compared to virus control. Our results have shown that Al2O3-NPs have a robust antiviral activity against HSV-1. This function demonstrates excellent potential for using Al2O3-NP in topical formulations for treating orolabial or genital herpetic lesions.
کلیدواژه‌ها
Herpes simplex virus type 1؛ Aluminum oxide nanoparticles؛ Real-Time PCR؛ Antiviral activity؛ Indirect immunofluorescence assay
سایر فایل های مرتبط با مقاله
مراجع
  1. Zhang J, Liu H, Wei B. Immune response of T cells during herpes simplex virus type 1 (HSV-1) infection. J Zhejiang Univ Sci B. 2017;18(4):277-88.
  2. Duarte LF, Farías MA, Álvarez DM, Bueno SM, Riedel CA, González PA. Herpes simplex virus type 1 infection of the central nervous system: insights into proposed interrelationships with neurodegenerative disorders. Front Cell Neurosci. 2019;13:46.
  3. WHO. Herpes simplex virus. 2022 [Available from: https://www.who.int/news-room/fact-sheets/detail/herpes-simplex-virus.
  4. Trigilio J, Antoine TE, Paulowicz I, Mishra YK, Adelung R, Shukla D. Tin oxide nanowires suppress herpes simplex virus-1 entry and cell-to-cell membrane fusion. PLoS One. 2012;7(10): 48147.
  5. Kongyingyoes B, Priengprom T, Pientong C, Aromdee C, Suebsasana S, Ekalaksananan T. 3, 19-isopropylideneandrographolide suppresses early gene expression of drug-resistant and wild type herpes simplex viruses. Antivir Res. 2016;132:281-6.
  6. Kakiuchi S, Tsuji M, Nishimura H, Yoshikawa T, Wang L, Takayama-Ito M, et al. Association of the emergence of acyclovir-resistant herpes simplex virus type 1 with prognosis in hematopoietic stem cell transplantation patients. J Infect Dis. 2017;215(6):865-73.
  7. Albukhaty S, Al-Musawi S, Abdul Mahdi S, Sulaiman GM, Alwahibi MS, Dewir YH, et al. Investigation of dextran-coated superparamagnetic nanoparticles for targeted vinblastine controlled release, delivery, apoptosis induction, and gene expression in pancreatic cancer cells. Molecules. 2020;25(20):4721.
  8. Al-Musawi S, Albukhaty S, Al-Karagoly H, Sulaiman GM, Jabir MS, Naderi-Manesh H. Dextran-coated superparamagnetic nanoparticles modified with folate for targeted drug delivery of camptothecin. Adv Nat Sci Nanosci Nanotechnol. 2020;11(4):045009.
  9. Haider AJ, Al-Kinani MA, Al-Musawi S, editors. Preparation and Characterization of Gold Coated Super Paramagnetic Iron Nanoparticle Using Pulsed Laser Ablation in Liquid Method. Key Engineering Materials; 2021: Trans Tech Publ.
  10. Xiang D-x, Chen Q, Pang L, Zheng C-l. Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro. J Virol Methods. 2011;178(1-2):137-42.
  11. Lu L, Sun RW-Y, Chen R, Hui C-K, Ho C-M, Luk JM, et al. Silver nanoparticles inhibit hepatitis B virus replication. Antivir Ther. 2008;13(2):253-62.
  1. Mahdi SA, Kadhim AA, Albukhaty S, Nikzad S, Haider AJ, Ibraheem S, et al. Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil. Electron J Biotechnol. 2021;52:21-9.
  2. Sezer N, Evis Z, Kayhan SM, Tahmasebifar A, Koç M. Review of magnesium-based biomaterials and their applications. Journal of magnesium and alloys. 2018;6(1):23-43.
  3. Al-Musawi S, Ibraheem S, Abdul Mahdi S, Albukhaty S, Haider AJ, Kadhim AA, et al. Smart nanoformulation based on polymeric magnetic nanoparticles and vincristine drug: a novel therapy for apoptotic gene expression in tumors. Life. 2021;11(1):71.
  4. Maruzuru Y, Shindo K, Liu Z, Oyama M, Kozuka-Hata H, Arii J, et al. Role of herpes simplex virus 1 immediate early protein ICP22 in viral nuclear egress. Virol J. 2014;88(13):7445-54.
  5. Dziuba N, Ferguson MR, O'Brien WA, Sanchez A, Prussia AJ, McDonald NJ, et al. Identification of cellular proteins required for replication of human immunodeficiency virus type 1. AIDS Res Hum Retrovir. 2012;28(10):1329-39.
  6. Zhang Q, Wang H, Ge C, Duncan J, He K, Adeosun SO, et al. Alumina at 50 and 13 nm nanoparticle sizes have potential genotoxicity. J Appl Toxicol. 2017;37(9):1053-64.
  7. Huet A, Huffman JB, Conway JF, Homa FL. Role of the herpes simplex virus CVSC proteins at the capsid portal vertex. Virol J. 2020;94(24): 01534-20.
  8. Ibáñez FJ, Farías MA, Gonzalez-Troncoso MP, Corrales N, Duarte LF, Retamal-Díaz A, et al. Experimental dissection of the lytic replication cycles of herpes simplex viruses in vitro. Front. Microbiol. 2018:2406.
  9. Carmody CM, Goddard JM, Nugen SR. Bacteriophage Capsid Modification by Genetic and Chemical Methods. Bioconjug Chem. 2021;32(3):466-81.
آمار
تعداد مشاهده مقاله: 954
تعداد دریافت فایل اصل مقاله: 281


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب