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Abbas Mohammed, A, Niamah, A. K. (1401). Production and Optimization of Hyaluronic Acid Extracted from Streptococcus thermophilus Isolates. سامانه مدیریت نشریات علمی, 77(6), 2395-2405. doi: 10.22092/ari.2022.358612.2262
A Abbas Mohammed; A. K Niamah. "Production and Optimization of Hyaluronic Acid Extracted from Streptococcus thermophilus Isolates". سامانه مدیریت نشریات علمی, 77, 6, 1401, 2395-2405. doi: 10.22092/ari.2022.358612.2262
Abbas Mohammed, A, Niamah, A. K. (1401). 'Production and Optimization of Hyaluronic Acid Extracted from Streptococcus thermophilus Isolates', سامانه مدیریت نشریات علمی, 77(6), pp. 2395-2405. doi: 10.22092/ari.2022.358612.2262
Abbas Mohammed, A, Niamah, A. K. Production and Optimization of Hyaluronic Acid Extracted from Streptococcus thermophilus Isolates. سامانه مدیریت نشریات علمی, 1401; 77(6): 2395-2405. doi: 10.22092/ari.2022.358612.2262

Production and Optimization of Hyaluronic Acid Extracted from Streptococcus thermophilus Isolates

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مقاله 47، دوره 77، شماره 6، بهمن و اسفند 2022، صفحه 2395-2405    اصل مقاله (738.21 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358612.2262
نویسندگان
A Abbas Mohammed* 1؛ A. K Niamah2
1Department of Marine Vertebrates, Marine Science Center, University of Basra, Basra, Iraq
2Faculty of Agriculture, Department of Food Sciences, University of Basra, Basra, Iraq
چکیده
Biopolymers, particularly exopolysaccharides produced by microorganisms such as bacteria, yeasts, and algae, have gained popularity in recent years due to their physical, chemical, and functional properties that are widely useful in food, industrial, cosmetic, and pharmaceutical systems. Hyaluronic acid is one type of these polysaccharide. This study investigated the optimal conditions for producing hyaluronic acid from the Streptococcus thermophilus bacterial strain. The isolated Streptococcus thermophilus were cultured on MRS broth, Skim milk, and M17 broth with an addition of 1% lactose. The diagnosed bacterial strains were grown in 100 ml of culture media, placed in volumetric flasks of 250 ml capacity, and incubated at 42˚C for 24 hours, pH 6.8, inoculum volume 1%, and a vibrating incubator at 150 rpm. After the end of the fermentation period, the isolation and purification of HA have performed accordingly: proteins were removed using 1% trichloroacetic acid (TCA), and HA in the supernatant was collected by isopropanol precipitation. The collected HA was dialyzed against ultrapure water and lyophilized. The amount of acid produced was estimated. The results show that the best production of hyaluronic acid was from the S. thermophilus bacterial strain grown on the alternative medium containing whey at a ratio of 450 ml/L and7.5 g/L yeast extract at 40 ˚C, with a 3% of inoculum volume and 102×108 colony-forming units/ml of bacterial cells, in pH 6.8 and agitation speed of 150 rpm for 18 h, which had the most significant effect on the fermentation process and gave the highest value of HA production of 0.598 g/L and biomass of 6.08 g/L. These results showed the best production method for HA to achieve maximal production yelled.
کلیدواژه‌ها
Hyaluronic Acid؛ Optimal Conditions؛ S. thermophilus Bacteria Strain
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مراجع
  1. Özcan E, Öner ET. Microbial production of extracellular polysaccharides from biomass sources. Polysaccharides: Bioact Biotechnol. 2015:161-84.
  2. de Oliveira JD, Carvalho LS, Gomes AMV, Queiroz LR, Magalhães BS, Parachin NS. Genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms. Microbial Cell Factories. 2016;15(1):1-19.
  3. Saraphanchotiwitthaya A, Sripalakit P. Production of Hyaluronic Acid from Molasses by Streptococcus thermophilus TISTR 458. Trends Sci. 2022;19(4):2192-.
  4. Armstrong D, Cooney M, Johns M. Growth and amino acid requirements of hyaluronic-acid-producing Streptococcus zooepidemicus. Appl Microbiol Biotechnol. 1997;47(3):309-12.
  5. Hutkins R, Goh Y. Streptococcus: Streptococcus thermophilus. Encyclopedia of Food Microbiology: Second Edition: Elsevier Inc.; 2014. p. 554-9.
  6. Pan N, Marques R, Pereira H, Vignoli J, Celligoi MA, editors. Effects of different nitrogen sources on the production of Hyaluronic acid by Streptococcus. BMC Proceedings; 2014: Springer.
  7. Amado IR, Vázquez JA, Pastrana L, Teixeira JA. Microbial production of hyaluronic acid from agro-industrial by-products: Molasses and corn steep liquor. Biochem Eng J. 2017;117:181-7.
  8. Gedikli S, Güngör G, Toptaş Y, Sezgin DE, Demirbilek M, Yazıhan N, et al. Optimization of hyaluronic acid production and its cytotoxicity and degradability characteristics. Prep Biochem Biotechnol. 2018;48(7):610-8.
  9. Tu NH, Trang PT, editors. Effects of Rice-Washing Water on the Hyaluronic Acid Production of StreptococcusThermophilus. 4th International Conference on Biomedical Engineering in Vietnam; 2013: Springer.
  10. Sharma R, Sanodiya BS, Thakur GS, Jaiswal P, Pal S, Sharma A, et al. Characterization of lactic acid bacteria from raw milk samples of cow, goat, sheep, camel and buffalo with special elucidation to lactic acid production. Br Microbiol Res J. 2013;3(4):743-52.
  11. Sheng J, Ling P, Wang F. Constructing a recombinant hyaluronic acid biosynthesis operon and producing food-grade hyaluronic acid in Lactococcus lactis. J Ind Microbiol Biotechnol. 2015;42(2):197-206.
  12. Pires AM, Macedo AC, Eguchi SY, Santana MH. Microbial production of hyaluronic acid from agricultural resource derivatives. Bioresour Technol. 2010;101(16):6506-9.
  13. Izawa N, Serata M, Sone T, Omasa T, Ohtake H. Hyaluronic acid production by recombinant Streptococcus thermophilus. J Biosci Bioeng. 2011;111(6):665-70.
  14. Mohammed AA, Niamah AK. Identification and antioxidant activity of hyaluronic acid extracted from local isolates of Streptococcus thermophilus. Mater Today. 2021.
  15. Sciabica S, Tafuro G, Semenzato A, Traini D, Silva DM, Reis LGD, et al. Design, Synthesis, Characterization, and In Vitro Evaluation of a New Cross-Linked Hyaluronic Acid for Pharmaceutical and Cosmetic Applications. Pharmaceutics. 2021;13(10):1672.
  16. Al-Roomi FW, Al-Sahlany ST. Identification and Characterization of Xanthan Gum Produced from Date Juice by a Local Isolate of Bacteria Xanthomonas campestris. Basrah J Agric Sci. 2022;35(1):35-49.
  17. Cao X, Cai C, Wang Y, Zheng X. The inactivation kinetics of polyphenol oxidase and peroxidase in bayberry juice during thermal and ultrasound treatments. Innov Food Sci Emerg Technol. 2018;45:169-78.
  18. Ma T, Wang J, Wang L, Yang Y, Yang W, Wang H, et al. Ultrasound-combined sterilization technology: An effective sterilization technique ensuring the microbial safety of grape juice and significantly improving its quality. Foods. 2020;9(10):1512.
  19. Kosikowski F, Mistry V. Cheese and Fermented Milk Products. vol. 1. FV Kosikowski, Westport, CT. 1997:328-52.
  20. Ranganna S. Manual of analysis of fruit and vegetable products. 1977.
  21. Cebeci A, Gürakan GC. Molecular methods for identification of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus using methionine biosynthesis and 16S rRNA genes. J Dairy Res. 2008;75(4):392-8.
  22. Chen Y-H, Li J, Liu L, Liu H-Z, Wang Q. Optimization of flask culture medium and conditions for hyaluronic acid production by a streptococcus equisimilis mutant nc2168. Braz J Microbiol. 2012;43(4):1553-61.
  23. Prasad SB, Jayaraman G, Ramachandran K. Hyaluronic acid production is enhanced by the additional co-expression of UDP-glucose pyrophosphorylase in Lactococcus lactis. Appl Microbiol Biotechnol. 2010;86(1):273-83.
  24. Lee G-Y, Ha S-J, Jung J-H, Seo D-H, Park J-Y, Kim S-R, et al. Effect of non-animal-derived nitrogen sources on the production of hyaluronic acid by Streptococcus sp. KL0188. J Korean Soc Appl Biol Chem. 2009;52(3):283-9.
  25. Chen S-J, Chen J-L, Huang W-C, Chen H-L. Fermentation process development for hyaluronic acid production by Streptococcus zooepidemicus ATCC 39920. Korean J Chem Eng. 2009;26(2):428-32.
  26. Lai Z-W, Rahim RA, Ariff A, Mohamad R. Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920. Afr J Microbiol Res. 2011;5(15):2114-23.
  27. Im J-H, Song J-M, Kang J-H, Kang D-J. Optimization of medium components for high-molecular-weight hyaluronic acid production by Streptococcus sp. ID9102 via a statistical approach. J Ind Microbiol Biotechnol. 2009;36(11):1337.
  28. Izawa N, Hanamizu T, Sone T, Chiba K. Effects of fermentation conditions and soybean peptide supplementation on hyaluronic acid production by Streptococcus thermophilus strain YIT 2084 in milk. J Biosci Bioeng. 2010;109(4):356-60.
  29. Shoparwe NF, Kew WS, Mohamad M, Ameram N, Makhtar MMZ, editors. Optimization And Kinetic Analysis On The Production Of Hyaluronic Acid By Streptococcus Zooepidemicus In A Batch System. IOP Conference Series: Earth and Environmental Science; 2020: IOP Publishing.
  30. Aroskar V, Kamat S, Kamat D. Effect of various physical parameters and statistical medium optimization on production of hyaluronic acid using S. equi subsp. zooepidemicus ATCC 39920. IIOAB. 2012;2(1):16-24.
  31. Jagannath S, Ramachandran K. Influence of competing metabolic processes on the molecular weight of hyaluronic acid synthesized by Streptococcus zooepidemicus. Biochem Eng J. 2010;48(2):148-58.
  32. Kumar A, Janakiraman S, Nataraj LK. Optimization study for enhanced production of hyaluronic acid from Streptococcus equisimilus MK156140. Korean J Chem Eng. 2021;38(9):1880-7.
  33. Hasegawa S, Nagatsuru M, Shibutani M, Yamamoto S, Hasebe S. Productivity of concentrated hyaluronic acid using a Maxblend® fermentor. J Biosci Bioeng. 1999;88(1):68-71.
  34. Huang W-C, Chen S-J, Chen T-L. The role of dissolved oxygen and function of agitation in hyaluronic acid fermentation. Biochem Eng J. 2006;32(3):239-43.
  1. Johns MR, Goh L-T, Oeggerli A. Effect of pH, agitation and aeration on hyaluronic acid production byStreptococcus zooepidemicus. Biotechnol Lett. 1994;16(5):507-12.
  2. Zhang X, Duan X-J, Tan W-S. Mechanism for the effect of agitation on the molecular weight of hyaluronic acid produced by Streptococcus zooepidemicus. Food Chem. 2010;119(4):1643-6.
  3. Zakeri A, Rasaee MJ. Identification of wild type Streptococcus zooepidemicus and optimization of culture medium and fermentation conditions for production of hyaluronic acid. Biosci Biotechnol Res Asia. 2016;13(1):189-98.
  4. Sunguroğlu C, Sezgin DE, Aytar Çelik P, Çabuk A. Higher titer hyaluronic acid production in recombinant Lactococcus lactis. Prep Biochem Biotechnol. 2018;48(8):734-42.
  5. Duffeck HCBP, Pan NC, Saikawa GIA, da Rocha SPD, Baldo C, Celligoi MAPC. Biomedical Potential of Hyaluronic Acid from Streptococcus zooepidemicus Produced in Sugarcane Molasses. Braz J Dev. 2020;6(7):49963-80.
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