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Production of camel-specific lactoferrin antibodies using Freund and chitosan adjuvants | ||
| علوم و فنون دامپروری | ||
| Volume 11, Issue 42, June 2022, Pages 55-62 PDF (1.21 M) | ||
| DOI: 10.22092/aasrj.2022.126942 | ||
| Authors | ||
| S. Zibaei* ; M. Farhang | ||
| Abstract | ||
| Lactoferrin has clinical and commercial applications. In the present study, ion exchange chromatography by CM Sephadex C-50 was used to purify lactoferrin and SDS-PAGE test was performed to confirm the absence of dye in the presence of tetramethyl benzene (TMB). In order to production of camel-specific lactoferrin antibodies, New Zealand white male rabbits were immunized with 120 µg/ml lactoferrin using Freund's and chitosan adjuvants for 6 weeks. Dot-blot tests were used to confirm and compare the amount of antibody produced. In the dot blot test, the formation of brown spots confirmed the binding of the antigen to the antibody, and the intensity of the brown color was related to the amount of antibody. ELISA results also showed the presence of high concentrations of antibodies. The results showed that the production of specific antibodies using chitosan adjuvant was higher than Freund's adjuvant with a significant difference of α <5%. | ||
| Keywords | ||
| Lactoferrin; Camel milk; camel-specific antibodies | ||
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