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Chayan, M, Al-Fahad, D, Al-Bedhawi, M. (1401). The Role of Akt/Rab5A Signalling in Regulating Cell Migration of MDA-MB-231 Breast Cancer Cell Line. سامانه مدیریت نشریات علمی, 78(1), 427-434. doi: 10.22092/ari.2022.358954.2341
M Chayan; D Al-Fahad; M Al-Bedhawi. "The Role of Akt/Rab5A Signalling in Regulating Cell Migration of MDA-MB-231 Breast Cancer Cell Line". سامانه مدیریت نشریات علمی, 78, 1, 1401, 427-434. doi: 10.22092/ari.2022.358954.2341
Chayan, M, Al-Fahad, D, Al-Bedhawi, M. (1401). 'The Role of Akt/Rab5A Signalling in Regulating Cell Migration of MDA-MB-231 Breast Cancer Cell Line', سامانه مدیریت نشریات علمی, 78(1), pp. 427-434. doi: 10.22092/ari.2022.358954.2341
Chayan, M, Al-Fahad, D, Al-Bedhawi, M. The Role of Akt/Rab5A Signalling in Regulating Cell Migration of MDA-MB-231 Breast Cancer Cell Line. سامانه مدیریت نشریات علمی, 1401; 78(1): 427-434. doi: 10.22092/ari.2022.358954.2341

The Role of Akt/Rab5A Signalling in Regulating Cell Migration of MDA-MB-231 Breast Cancer Cell Line

Archives of Razi Institute
مقاله 53، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 427-434    اصل مقاله (774.57 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358954.2341
نویسندگان
M Chayan* 1؛ D Al-Fahad1، 2؛ M Al-Bedhawi3
1Department of Basic Sciences, College of Dentistry, University of Thi-Qar, Thi-Qar 64001, Iraq
2Department of Pharmaceutical Sciences, College of Pharmacy University of Thi-Qar, Thi-Qar 64001, Iraq
3Institute of Genetic Engineering and Biotechnology, University of Baghdad, Baghdad, Iraq
چکیده
Rab5A and Akt pathways are reported to be responsible for the invasiveness of cancer cells, indicated by the fact that Rab5A activates the downstream Phosphoinositide-3-kinases (PI3K)/Akt signalling pathway, which results in promoting cancer metastasis. However, little attention has been given to the emerging role of Rab5A and Akt signalling pathways in regulating the direction of MDA-MB-231 cell migration. MDA-MB-231 breast cancer cell line was used as a model in this study because it is highly metastatic and motile. Time-lapse microscopy was used to examine the effect of Akt and Rab5A inhibitors on cell migration, proliferation and wound healing. Later, the cells were transfected with GFP-Akt-PH or GFP-Rab5A (used as a biosensor to detect Akt and Rab5A). Therefore, confocal time-lapse images were used to visualize Akt and Rab5A at the front and rear edges of the cells. The recorded data demonstrated that Akt and Rab5A inhibition reduced cell migration, proliferation and wound healing. The results of the current study also demonstrated that Akt localizes at the trailing edge while Rab5A localize more at the leading edge than the trailing edge of cells. This study suggests that Akt and Rab5A inhibition might regulate the direction of breast cancer migration.
کلیدواژه‌ها
Akt؛ Rab5A؛ Cancer Cell Migration؛ MDA-MB-231 Cell Line
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مراجع
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