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Miri Mizher Al Muhana, Balsam, ‬‬‬‬‬‬‬‬‬‬ A. Ali, Saba, Sabri Jabbar, Ali. (1401). Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-19 Patients with Molecular Detection of Antibiotics Resistance Genes. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2022.359035.2360
Balsam Miri Mizher Al Muhana; Saba ‬‬‬‬‬‬‬‬‬‬ A. Ali; Ali Sabri Jabbar. "Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-19 Patients with Molecular Detection of Antibiotics Resistance Genes". سامانه مدیریت نشریات علمی, , , 1401, -. doi: 10.22092/ari.2022.359035.2360
Miri Mizher Al Muhana, Balsam, ‬‬‬‬‬‬‬‬‬‬ A. Ali, Saba, Sabri Jabbar, Ali. (1401). 'Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-19 Patients with Molecular Detection of Antibiotics Resistance Genes', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2022.359035.2360
Miri Mizher Al Muhana, Balsam, ‬‬‬‬‬‬‬‬‬‬ A. Ali, Saba, Sabri Jabbar, Ali. Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-19 Patients with Molecular Detection of Antibiotics Resistance Genes. سامانه مدیریت نشریات علمی, 1401; (): -. doi: 10.22092/ari.2022.359035.2360

Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-19 Patients with Molecular Detection of Antibiotics Resistance Genes

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 19 تیر 1401  XML
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359035.2360
نویسندگان
Balsam Miri Mizher Al Muhana email 1؛ Saba ‬‬‬‬‬‬‬‬‬‬ A. Ali2؛ Ali Sabri Jabbar2
1Veterinary Microbiology Dept., College of Veterinary Medicine, Al-Qadisiyah
2Clinical Laboratory Sciences Dept., College of Pharmacology, Al-Qadisiyah University
چکیده
Pseudomonas aeruginosa (P. aeruginosa) have a considerable risk to public health in the world, due to its high ability to develop resistance to different classes of antibiotics. It has been discovered as a prevalent coinfection pathogen that causes sickness exacerbation in COVID-19 patients. This study aimed to determine the prevalence of P. aeruginosa from COVID-19 patients in Al Diwaniyah province, Iraq and to identify its genetic resistance pattern. 70 clinical samples were obtained from severe cases of patients (RT-PCR positive for SARS-COV-2 on a nasopharyngeal swab) who attended Al Diwaniyah Academic Hospital. 50 P. aeruginosa bacterial isolates were detected via microscopic examination, routine cultured and biochemical testing, then validated by the VITEK-2 compact system. VITEK reported 30 positive results, which later confirmed through molecular detection using 16s RNA specific for detection and a phylogenetic tree.20 isolates had positive PCR findings and 5 isolates submitted to GenBank with accession numbers OL314557.1, OL314556.1, OL314555.1, OL314554.1, OL314553.1.For antibiotic resistance genes, the number of the isolates containing blaOXA-1 and blaCTX-M 18 (90 percent) and 16 (80 percent) respectively. To study its adaptation in a SARS-CoV-2 infected environment, genomic sequencing investigations were undertaken with phenotypic validation. In conclusion, we demonstrate that multidrug resistant P. aeruginosa play an important role in in vivo colonization in COVID-19 patients and could be one of the causes of death of these patients which indicates the great challenge to clinicians in the facing of this serious disease.
کلیدواژه‌ها
Pseudomonas aeruginosa؛ bla OXA-1, bla CTX-M؛ antibiotic resistance؛ coinfection
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تعداد مشاهده مقاله: 88


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