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Ghazy Hawal, R, Talib Bakr, M. (1402). Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields. سامانه مدیریت نشریات علمی, 78(2), 587-592. doi: 10.22092/ari.2022.359468.2424
R Ghazy Hawal; M Talib Bakr. "Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields". سامانه مدیریت نشریات علمی, 78, 2, 1402, 587-592. doi: 10.22092/ari.2022.359468.2424
Ghazy Hawal, R, Talib Bakr, M. (1402). 'Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields', سامانه مدیریت نشریات علمی, 78(2), pp. 587-592. doi: 10.22092/ari.2022.359468.2424
Ghazy Hawal, R, Talib Bakr, M. Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields. سامانه مدیریت نشریات علمی, 1402; 78(2): 587-592. doi: 10.22092/ari.2022.359468.2424

Isolation and Molecular Identification of Salmonella pullorum from Broiler Chicken in Iraqi Fields

Archives of Razi Institute
مقاله 11، دوره 78، شماره 2، خرداد و تیر 2023، صفحه 587-592    اصل مقاله (648.72 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359468.2424
نویسندگان
R Ghazy Hawal* ؛ M Talib Bakr
Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
چکیده
Pullorum disease (PD) is one of the most common diseases in the world, with devastating consequences. In the chicken sector, there have been financial losses. It is brought on by Salmonella enteric subspecies serovar Gallinarum biovar pullorum; definitive detection requires culture followed by biochemistry analysis and serotyping. This study aimed to verify the presence of bacteria by culture, biochemical characterization, PCR assay, and sequencing. One hundred samples were collected from 12 broiler chicken flocks of different ages for 8districts of Baghdad province, including cloacal swabs (65), visceral organs (15), and dropping (20). Salmonella colonies were identified by selective culture broth and agar with biochemical description for 75% of the total samples, with a higher incidence in visceral organs than dropping and cloacal swabs. ،The Sequencing and phylogenetic tree analysis of 16S rRNA gene for representative Salmonella isolates. The presence of Salmonella pullorum isolates in global genetic strains; was revealed a matching NCBI isolates similarity of 99.02% with (MF445124.1) and 98% with (MH352164.1), respectively. In the current state of molecular and genetic research, phlyogentic research announced the real presence of Salmonella pullorum in Baghdadprovince's broiler chicken, also showing the phylogentic characteristics and links to some global isolates. The detection of Salmonella pullorum in broiler flocks of the current study extent of health risks to other uninfected birds present in the free range.
کلیدواژه‌ها
Salmonella pullorum؛ Biochemical Tests؛ PCR؛ Phylogenetic Analysis
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مراجع
  1. Brooks BW, Lutze-Wallace CL, Blais B, Gauthier M, Deschenes M. Monoclonal antibodies to lipopolysaccharide O antigens of enterohemorrhagic Escherichia coli strains in serogroups O26, O45, O103, O111, O121, and O145. J Food Prot. 2015;78(7):1252-8.
  2. Revolledo L. Vaccines and vaccination against fowl typhoid and pullorum disease: An overview and approaches in developing countries. J Appl Poult Res. 2018;27(3):279-91.
  3. Sharif YM, Tayeb BA. Estimation of limit of detection of Salmonella typhimurium in artificially contaminated chicken meat by cultured-based and polymerase chain reaction techniques. Iraqi J Vet Sci. 2021;35(4):621-5.
  4. Sabri JB, Al-Sultan II, Altaif K, Peter S, Saadh MJ. Pathogenesis of Salmonella enterica serovar Albany in experimental infected SPF BALB/c Mice. Iraqi J Vet Sci. 2020;34(2):339-44.
  5. Xu Y, Hu Y, Guo Y, Zhou Z, Xiong D, Meng C, et al. A new PCR assay based on the new gene-SPUL_2693 for rapid detection of Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum. Poult Sci. 2018;97(11):4000-7.
  6. Siala M, Barbana A, Smaoui S, Hachicha S, Marouane C, Kammoun S, et al. Screening and detecting Salmonella in different food matrices in Southern Tunisia using a combined enrichment/real-time PCR method: Correlation with conventional culture method. Front Microbiol. 2017;8:2416.
  7. Markey B, Leonard F, Archambault M, Cullinane A, Maguire D. Clinical veterinary microbiology e-book: Elsevier Health Sciences; 2013.
  8. Moore E, Arnscheidt A, Krüger A, Strömpl C, Mau M. Simplified protocols for the preparation of genomic DNA from bacterial cultures. MMEM. 1999;1(1):1-15.
  9. Ojha SC, Yean Yean C, Ismail A, Banga Singh K-K. A pentaplex PCR assay for the detection and differentiation of Shigella species. BioMed Res Int. 2013;2013.
  10. AL-Iedani AA, Khudor MH, Oufi NM. Isolation and identification of Salmonella spp. from poultry farms by using different techniques and evaluation of their antimicrobial susceptibilities. Bas J Vet Res. 2014;1(1):246-59.
  11. Al-Niema A, Youkhana S. Survey on the most important diseases of the digestive system in laying hens and parent stock in Mosul. Iraqi J Vet Sci. 2012;26:241-5.
  12. Ayesha K, Mahmood M, Iftikhar H, Faisal S, Azhar R, Asif I, et al. Bacteriological and epidemiological investigations of Pullorum disease in selected poultry farms of faisalabad, Pakistan. Glob Vet. 2014;12(4):455-60.
  13. Rumi N, Rahman M, Akter M, Fakhruzzaman M, Hossain M. Evaluation of the effectiveness of commercially available disinfectants against salmonellae isolated from internal organs of dead chickens. Bangladesh J Vet Med. 2011;9(1):43-52.
  14. Ashraf A, Ahmed MA, Aisha RA, Fatma I, Mohammed E. Detection of common (inv A) gene in salmonellae isolated from poultry using polymerase chain rection technique. Benha Vet Med J. 2013;25:70-7.
  15. Abd El-Ghany WA, El-Shafii SS, Hatem M. A survey on Salmonella species isolated from chicken flocks in Egypt. Asian J Anim Vet Adv. 2012;7(6):489-501.
  16. Xiong D, Song L, Geng S, Tao J, An S, Pan Z, et al. One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB. Front Microbiol. 2016;7:1863.
  17. Saud BH, AL-Zuhariy MT. Using T cell lymphokines to enhance the immune response against Newcastle disease in vaccinated broiler chickens fed naturally contaminated diet with different mycotoxins. Iraqi J Vet Sci. 2020;34(2):427-33.
  18. Majchrzak M, Krzyzanowska A, Kubiak AB, Wojtasik A, Wolkowicz T, Szych J, et al. TRS-based PCR as a potential tool for inter-serovar discrimination of Salmonella Enteritidis, S. Typhimurium, S. Infantis, S. Virchow, S. Hadar, S. Newport and S. Anatum. Mol Biol Rep. 2014;41(11):7121-32.
  1. Zou Q-H, Li R-Q, Liu G-R, Liu S-L. Genotyping of Salmonella with lineage-specific genes: correlation with serotyping. Int J Infect Dis. 2016;49:134-40.
  2. Feng Y, Johnston RN, Liu G-R, Liu S-L. Genomic comparison between Salmonella Gallinarum and Pullorum: differential pseudogene formation under common host restriction. PLoS One. 2013;8(3):59427.
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