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Bioinformatic Analysis and Sequencing of OmpL1-LipL41 Leptospiral Fusion Protein as a New Candidate for Leptospirosis Vaccine | ||
| تحقیقات دامپزشکی و فرآوردههای بیولوژیک | ||
| Article 15, Volume 36, Issue 2, June 2023, Pages 128-138 PDF (2.77 M) | ||
| Document Type: Short Communication | ||
| DOI: 10.22092/vj.2022.358520.1968 | ||
| Authors | ||
| melisa eghbal1; Fereshteh Ghandehari* 2; Pejvak Khaki3; Morteza Taghizadeh4; Majid Tebianian5 | ||
| 1Department of biology , falavarjan Branch , Islamic Azad University , Isfahan , Iran | ||
| 2Department of Microbiology, falavarjan Branch, Islamic Azad University, Isfahan, Iran | ||
| 3Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 4Department of Human vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 5Department of Immunology, Razi Vaccine and Serum Research Institute, Agricultural Research,Education and Extension Organization (AREEO), Karaj, Iran | ||
| Abstract | ||
| In recent years, due to advances in vaccine design, bioinformatic studies on Leptospira have undergone significant changes and advances. The aim of this study was to design a fusion protein based on OmpL1 and LipL41 surface proteins that have a conserved structure over time. Based on the previous field studies, the target proteins were examined and the OmpL1-LipL41 fusion protein was selected. After examining the chemical properties of the target proteins using appropriate servers, the initial structure of the fusion protein was determined and finally this protein was placed in the PET32a+ vector between the two enzymatic regions of HindIII and BamHI. The results revealed that the protein weighs 73 kDa and has 2097 bp and 610 amino acids. The isoelectric pH of the above protein is 7 and its solubility degree in the prokaryotic system is 100%. Finally, according to the above data, it is predicted that the designed construct structure has acceptable immunogenic properties and can be expressed to an acceptable and successful extent in prokaryotic systems and used for immunogenicity studies of Leptospira as a new candidate in the production of leptospirosis vaccine and in the design and manufacture of diagnostic kits. | ||
| Keywords | ||
| Prokaryote; Vector; Surface Protein | ||
| References | ||
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1- Bierque, E., R. Thibeaux, D. Girault, M.-E. Soupé-Gilbert and C. Goarant. 2020. A systematic review of Leptospira in water and soil environments. PLoS One 15: e0227055. 2- Boey, K., K. Shiokawa and S. Rajeev. 2019. Leptospira infection in rats: A literature review of global prevalence and distribution. PLoS neglected tropical diseases 13: 0007499. 3- Bourhy, P., L. Collet, S. Brisse and M. Picardeau. 2014. Leptospira mayottensis sp. nov., a pathogenic species of the genus Leptospira isolated from humans. International journal of systematic and evolutionary microbiology 64: 4061. 4- Christopher, W., B. Adler and S. Faine. 1982. Immunogenicity of leptospiral vaccines grown in protein-free medium. Journal of medical microbiology 15: 493-501. 5- Chu, K. M., R. Rathinam, P. Namperumalsamy and D. Dean. 1998. Identification of Leptospira species in the pathogenesis of uveitis and determination of clinical ocular characteristics in south India. The Journal of infectious diseases 177: 1314-1321. 6- Dezhbord, M., M. Esmaelizad, P. Khaki, F. Fotohi and A. Z. Moghaddam. 2014. Molecular identification of the ompL1 gene within Leptospira interrogans standard serovars. The Journal of Infection in Developing Countries 8: 688-693. 7- Dong, H., Y. Hu, F. Xue, D. Sun, D. M. Ojcius, Y. Mao and J. Yan. 2008. Characterization of the ompL1 gene of pathogenic Leptospira species in China and cross-immunogenicity of the OmpL1 protein. BMC microbiology 8: 1-12. 8- Golab, N., P. Khaki, N. Harzandi, M. Esmaelizad and M. Tebianian. 2020. Expression and purification of the Lipl41, a surface-exposed lipoprotein antigen of pathogenic Leptospira spp. Veterinarski arhiv 90: 297-305. 9- Haake, D., C. Champion, C. Martinich, E. Shang, D. Blanco, J. Miller and M. Lovett. 1993. Molecular cloning and sequence analysis of the gene encoding OmpL1, a transmembrane outer membrane protein of pathogenic Leptospira spp. Journal of bacteriology 175: 4225-4234. 10- Haake, D. A., M. K. Mazel, A. M. McCoy, F. Milward, G. Chao, J. Matsunaga and E. A. Wagar. 1999. Leptospiral outer membrane proteins OmpL1 and LipL41 exhibit synergistic immunoprotection. Infection and immunity 67: 6572-6582. 11- Kamaruzaman, I. N. A. 2019. Delineating host-pathogen interaction of pathogenic Leptospira spp. The University of Liverpool (United Kingdom). 12- Khaki, P., S. Moradi Bidhendi, Y.-F. Chang, M. S. Soltani and K. Tadaion. 2016. Amplification and cloning of a gene encoding a 41 kDa outer membrane protein (LipL41) of Leptospira interrogans serovar Canicola. Infection Epidemiology and Microbiology 2: 5-7. 13- Larentis, A. L., J. F. M. Q. Nicolau, G. dos Santos Esteves, D. T. Vareschini, F. V. R. de Almeida, M. G. dos Reis, R. Galler and M. A. Medeiros. 2014. Evaluation of pre-induction temperature, cell growth at induction and IPTG concentration on the expression of a leptospiral protein in E. coli using shaking flasks and microbioreactor. BMC research notes 7: 1-13. 14- Lehmann, J. S., M. A. Matthias, J. M. Vinetz and D. E. Fouts. 2014. Leptospiral pathogenomics. Pathogens 3: 280-308. 15- Lin, X. a., A. Sun, P. Ruan, Z. Zhang and J. Yan. 2011. Characterization of Conserved Combined T and B Cell Epitopes in Leptospira interrogansMajor Outer Membrane Proteins OmpL1 and LipL41. BMC microbiology 11: 1-9. 16- Mason, M. R., C. Encina, S. Sreevatsan and C. Muñoz-Zanzi. 2016. Distribution and diversity of pathogenic Leptospira species in peri-domestic surface waters from south central Chile. PLoS neglected tropical diseases 10: e0004895. 17- Mb, T., T. Af and N. Alto. 2021. The leptospiral LipL21 and LipL41 proteins exhibit a broad spectrum of interactions with host cell components. Virulence 12: 2798-2813. 18- Nally, J. E., J. P. Whitelegge, S. Bassilian, D. R. Blanco and M. A. Lovett. 2007. Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection. Infection and immunity 75: 766-773. 19- Slamti, L., M. A. de Pedro, E. Guichet and M. Picardeau. 2011. Deciphering morphological determinants of the helix-shaped Leptospira. Journal of bacteriology 193: 6266-6275. 20- Sørensen, H. P. and K. K. Mortensen. 2005. Advanced genetic strategies for recombinant protein expression in Escherichia coli. Journal of biotechnology 115: 113-128. 21- Vedhagiri, K., K. Natarajaseenivasan, P. Chellapandi, S. Prabhakaran, J. Selvin, S. Sharma and P. Vijayachari. 2009. Evolutionary implication of outer membrane lipoprotein-encoding genes ompL1, lipL32 and lipL41 of pathogenic Leptospira species. Genomics, proteomics & bioinformatics 7: 96-106. 22- Vincent, A. T., O. Schiettekatte, C. Goarant, V. K. Neela, E. Bernet, R. Thibeaux, N. Ismail, M. K. N. Mohd Khalid, F. Amran and T. Masuzawa. 2019. Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics. PLoS neglected tropical diseases 13: e0007270. 23- Wang, Z., L. Jin and A. Węgrzyn. 2007. Leptospirosis vaccines. Microbial cell factories 6: 1-10. 24- Yang, H.-L., Y.-Z. Zhu, J.-H. Qin, P. He, X.-C. Jiang, G.-P. Zhao and X.-K. Guo. 2006. In silico and microarray-based genomic approaches to identifying potential vaccine candidates against Leptospira interrogans. BMC genomics 7: 1-12. 25- ZHANG, X. Y., Y. Yu, P. He, Y. X. ZHANG, B. Y. HU, Y. Yang, Y. X. NIE, X. G. JIANG, G. P. ZHAO and X. K. GUO. 2005. Expression and comparative analysis of genes encoding outer membrane proteins LipL21, LipL32 and OmpL1 in epidemic leptospires. Acta biochimica et biophysica Sinica 37: 649-656. | ||
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