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Ali Fadhil, I, Qasim Hasan Alsaadi, B. (1402). Documentation of Genetic Diversity by Insulin-Like Growth Factor1 Receptor (Exon2) Gene for Fallow Deer (Dama dama) in Iraq. سامانه مدیریت نشریات علمی, 78(2), 633-642. doi: 10.22092/ari.2022.359477.2429
I Ali Fadhil; B Qasim Hasan Alsaadi. "Documentation of Genetic Diversity by Insulin-Like Growth Factor1 Receptor (Exon2) Gene for Fallow Deer (Dama dama) in Iraq". سامانه مدیریت نشریات علمی, 78, 2, 1402, 633-642. doi: 10.22092/ari.2022.359477.2429
Ali Fadhil, I, Qasim Hasan Alsaadi, B. (1402). 'Documentation of Genetic Diversity by Insulin-Like Growth Factor1 Receptor (Exon2) Gene for Fallow Deer (Dama dama) in Iraq', سامانه مدیریت نشریات علمی, 78(2), pp. 633-642. doi: 10.22092/ari.2022.359477.2429
Ali Fadhil, I, Qasim Hasan Alsaadi, B. Documentation of Genetic Diversity by Insulin-Like Growth Factor1 Receptor (Exon2) Gene for Fallow Deer (Dama dama) in Iraq. سامانه مدیریت نشریات علمی, 1402; 78(2): 633-642. doi: 10.22092/ari.2022.359477.2429

Documentation of Genetic Diversity by Insulin-Like Growth Factor1 Receptor (Exon2) Gene for Fallow Deer (Dama dama) in Iraq

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مقاله 17، دوره 78، شماره 2، خرداد و تیر 2023، صفحه 633-642    اصل مقاله (680.68 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359477.2429
نویسندگان
I Ali Fadhil* 1؛ B Qasim Hasan Alsaadi2
1Department of Animal Production, College of Agriculture, Al-Qasim Green University, 51013, Babylon, Iraq
2Institute of Genetic Engineering and Biotechnology for Post Graduate Studies, Baghdad University, Baghdad, Iraq
چکیده
Insulin-Like Growth Factor1 Receptor (Exon2) (IGF1R) gene plays a vital role in physiological impacts, such as growth, development, reproduction, and metabolism. A significant difference was noted between the IGR1R (exon 2) gene and the body weight of Dama dama. In addition, the heterozygosity pattern (AB) was significantly higher than the other pattern (AA). There are three single nucleotide polymorphisms (SNPs; 144G˃C, 147A˃G, and 210A˃C) within the IGF-1R (exon 2) locus. The statistical analyses indicated the presence of three different haplotypes (GAA, CAA, and GGC). The analysis of relative frequencies indicated that the most frequent haplotype in the studied Dama dama population was Hap3 (GGC) (43.4782%) out of the three observed haplotypes. The results of SSCP-PCR revealed the variability of the target gene between the genotype frequencies in Fallow deer (Dama dama) with a high level of significance (P≤0.01) with two patterns (AA and AB) and an absence of BB pattern. The allele frequency of AA record a high level (71.74%) than the other genotype (AB) (28.26%), with a high-frequency level of the A allele (0.86) than the B allele (0.14). In current findings, SSCP genotyped in the Dama dama DNA observed an estimated 72% monomorphic loci and 28% polymorphic loci approximately. Hardy Weinberg equilibrium test (HW) was applied to the SSCP-PCR data matrix, and the statistical test was based on a chi-square (χ2) test. Chi-square was (55.928%) with a highly significant level (P≤0.01) recorded in the present study. As related to AA and AB genotypes mean,  a significant difference (P≤0.05) was noted between IGF1R (exon 2) gene with a body weight of Dama dama, as well as the heterozygosity pattern (AB), was significantly (P≤0.05) higher than the other pattern (AA) (30.34±3.01kg versus 24.85±1.94kg), respectively. A significant impact (P≤0.05) between IGF1R (exon2) polymorphism and heart girth was founded to be related to the AB pattern (heterozygous) (76.92 ± 3.20 cm), whereas the lower value was related to the AA pattern (71.33 ± 2.49 cm). No significant differences in effects were shown in relation to body length and height at the shoulder. The present study is also interested in genetic characterization by calculating (Ne) as a tool for genetic diversity. Therefore, the number of alleles detected (Na) indicates that two alleles only were unique in the population of the study, with (1.3204) being the number of efficient alleles (Ne). Moreover, Shannon's Information index was recorded at 0.4073. The observed homozygosity (O.Hom.) and heterozygosity (HO) were (0.7174 and 0.2826), respectively. The values of expected homozygosity (E.Hom.) and heterozygosity (HE) were 0.7547 and 0.2453, respectively. The genetic diversity of Nei was 0.2427. The results showed an unexpected influx of IGF1R diversity measured by Fis and recorded the value (- 0.1646). In this sense, the results of the current study may be considered an approximation to the total genetic diversity of the population of Dama dama in Iraq, but the information obtained is relevant to proposing the strategies of conservation for the genetic diversity observed.
کلیدواژه‌ها
Fallow deer (Dama dama)؛ IGF1R (exon2) gene؛ SNPs؛ SSCP-PCR؛ Shannon's index؛ Gene flow
مراجع
  1. Bana NÁ, Nyiri A, Nagy J, Frank K, Nagy T, Stéger V, et al. The red deer Cervus elaphus genome CerEla1. 0: sequencing, annotating, genes, and chromosomes. Mol Genet Genom. 2018;293(3):665-84.
  2. Jensz K, Finley L. Species profile for the fallow deer, Dama dama. Latitude; 2013.
  1. Sarkar MS, Segu H, Bhaskar J, Jakher R, Mohapatra S, Shalini K, et al. Ecological preferences of large carnivores in remote, high-altitude protected areas: insights from Buxa Tiger Reserve, India. Oryx. 2018;52(1):66-77.
  2. Halmos T, Suba I. The physiological role of growth hormone and insulin-like growth factors. Orvosi Hetilap. 2019;160(45):1774-83.
  3. Dakheel MH. Genetic Effect for Responsible gene on Fat percentage in Milk of Friesian Crossbred with Local Breed in Babylon City. Euphrates J Agric Sci. 2017;9.
  4. Dakheel MH, al-Anbari NN. Effect of IGF2 Polymorphism on Body Weight and Body Dimensions of Fallow Deer in Iraq.
  5. Fadhil I. Genetic polymorphisms of CSN3 gene and its effect on some production traits. Iraqi J Agric Sci. 2019;2(50).
  6. Al-Samerria S, Radovick S. The role of insulin-like growth factor-1 (IGF-1) in the control of neuroendocrine regulation of growth. Cells. 2021;10(10):2664.
  7. AFRIANI T, PUTRA DE. Association of GH, IGF1R, and PIT1 genes polymorphism with average daily gain and body measurement in Pesisir cattle. Nus Biosci. 2018;10(4):221-5.
  8. Nieto-Estévez V, Defterali Ç, Vicario-Abejón C. IGF-I: a key growth factor that regulates neurogenesis and synaptogenesis from embryonic to adult stages of the brain. Front Neurosci. 2016;10:52.
  9. Cohick W. Physiology and endocrinology symposium: Effects of insulin on mammary gland differentiation during pregnancy and lactation. J Anim Sci. 2016;94(5):1812-20.
  10. Hu W, Li T, Hu R, Wu L, Li M, Meng X. MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene. Growth Factors. 2014;32(1):27-33.
  11. Sambrook J. A laboratory manual. Mol Cloning. 2001;1.
  12. Yang F, Zhang P, Huo L, Riaz H, Yang L, Zhang S, et al. The association of single nucleotide polymorphism in the IGF1, IGF2 and IGF1R with antler yield in Sika deer. Insulin. 2014.
  13. Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci U S A. 1989;86(8):2766-70.
  14. Byun SO, Fang Q, Zhou H, Hickford J. An effective method for silver-staining DNA in large numbers of polyacrylamide gels. Anal Biochem. 2009;385(1):174-5.
  15. Cary N. Statistical analysis system, User's guide. Statistical. Version 9. SAS Inst Inc USA. 2012.
  16. Duncan DB. Multiple range and multiple F tests. Biometrics. 1955;11(1):1-42.
  17. Hill WG, Mackay TF. DS Falconer and Introduction to quantitative genetics. Genetics. 2004;167(4):1529-36.
  18. Hunley KL, Cabana GS, Long JC. The apportionment of human diversity revisited. Am J Phys Anthropol. 2016;160(4):561-9.
  19. Nei M. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci U S A. 1973;70(12):3321-3.
  20. Szewczuk M. Effects of SNP within exon 7 of the ınsulin-like growth factor receptor type 1 (IGF1R) gene on growth traits in Angus cows. 2016.
  21. Colitti M. Distribution of BDNF and TrkB isoforms in growing antler tissues of red deer. Ann Anat. 2017;213:33-46.
  22. Castillo-Rodríguez RG, Serna-Lagunes R, Cruz-Romero A, Núñez-Pastrana R, Rojas-Avelizapa LI, Régulo CL-H, et al. Characterization of the genetic diversity of a population of Odocoileus virginianus veraecrucis in captivity using microsatellite markers. Neotropical Biol Conserv. 2020;15(1):29-41.
  23. Nelson SL, Taylor SA, Reuter JD. An isolated white‐tailed deer (Odocoileus virginianus) population on St. John, US Virgin Islands shows low inbreeding and comparable heterozygosity to other larger populations. Ecol Evol. 2021;11(6):2775-81.
  24. Dakheel MH, al-Anbari NN. Expression of Insulin Like Growth Factor One Restorer (IGF1R) Gene and Impact on Iraqi Fallow Deer Performance.
  25. Luo J, Qin F, Deng C, Li F, Li W, Yue X. Polymorphisms of IGF-IR gene and their association with economic traits in two indigenous Chinese dairy goat breeds. Gene. 2019;695:51-6.
  26. Wang W, Ouyang K, Su X, Xu M, Shangguan X. Polymorphism of insulin-like growth factor 1 receptor gene in 12 pig breeds and its relationship with pig performance traits. Asian-Australas J Anim Sci. 2006;19(11):1541-5.
  27. Kimura M, Crow JF. The number of alleles that can be maintained in a finite population. Genetics. 1964;49(4):725.
  28. Ambriz-Morales P, De La Rosa-Reyna XF, Sifuentes-Rincon AM, Parra-Bracamonte GM, Villa-Melchor A, Chassin-Noria O, et al. The complete mitochondrial genomes of nine white-tailed deer subspecies and their genomic differences. J Mammal. 2016;97(1):234-45.
  29. Kollars P, Beck M, Mech S, Kennedy P, Kennedy M. Temporal and spatial genetic variability in white-tailed deer (Odocoileus virginianus). Genetica. 2004;121(3):269-76.
  30. Hennecke M. Matching molecular genetics and morphology in the genus Ophrys. GIROS Orchidee Spontanee d’Europa. 2016;59:5-34.
  1. Newbolt CH, Acker PK, Neuman TJ, Hoffman SI, Ditchkoff SS, Steury TD. Factors influencing reproductive success in male white‐tailed deer. J Wildl Manage. 2017;81(2):206-17.
  2. Latch EK, Gee KL, Webb SL, Honeycutt RL, DeYoung RW, Gonzales RA, et al. Genetic consequences of fence confinement in a population of white-tailed deer. Diversity. 2021;13(3):126.
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