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Hakim Abed Bresm, M, Mohammed Hassan Habeeb, H. (1402). Effect of Vitamin D3 on some Antioxidant Parameters in Chilled Semen in Awassi Ram. سامانه مدیریت نشریات علمی, 78(2), 681-687. doi: 10.22092/ari.2022.359482.2433
M Hakim Abed Bresm; H Mohammed Hassan Habeeb. "Effect of Vitamin D3 on some Antioxidant Parameters in Chilled Semen in Awassi Ram". سامانه مدیریت نشریات علمی, 78, 2, 1402, 681-687. doi: 10.22092/ari.2022.359482.2433
Hakim Abed Bresm, M, Mohammed Hassan Habeeb, H. (1402). 'Effect of Vitamin D3 on some Antioxidant Parameters in Chilled Semen in Awassi Ram', سامانه مدیریت نشریات علمی, 78(2), pp. 681-687. doi: 10.22092/ari.2022.359482.2433
Hakim Abed Bresm, M, Mohammed Hassan Habeeb, H. Effect of Vitamin D3 on some Antioxidant Parameters in Chilled Semen in Awassi Ram. سامانه مدیریت نشریات علمی, 1402; 78(2): 681-687. doi: 10.22092/ari.2022.359482.2433

Effect of Vitamin D3 on some Antioxidant Parameters in Chilled Semen in Awassi Ram

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مقاله 23، دوره 78، شماره 2، خرداد و تیر 2023، صفحه 681-687    اصل مقاله (495.71 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359482.2433
نویسندگان
M Hakim Abed Bresm؛ H Mohammed Hassan Habeeb*
Department of Animal Production, College of Agriculture, Al-Qasim Green University, Babylon, Iraq
چکیده
To sustain the viability of the sperm of farm animals, the sperm is chilled. However, reactive oxygen species (ROS) may damage it, resulting in oxidative stress and decreased sperm viability. This study aimed to assess the various concentrations of vitamin D3 as antioxidants in the chilled sperm of Awassi. This study was performed on 23 ejaculates from three Awassi rams. The samples were combined, diluted with Tris-egg yolk extender (1:10), and then divided into aliquots. Aliquots were treated with three vitamin D3 concentrations (T1=0.02, T2=0.004, and T3=0.002 g/ml) and one control without the addition of vitamin D3. The experimental and control groups were chilled to reach 5 ºC. Following treatment, the samples were centrifuged at 2,000 RPM for 20 min at 0 and 72 h after the treatment. Until evaluation, the seminal plasm was stored in a freezer at 20 ºC. In this study, the antioxidant activity of vitamin D3 was evaluated using malondialdehyde (MDA), ROS, total antioxidant capacity (TAC), superoxide dismutase (SOD), and catalase (CAT). The SAS software was used to analyze variance on repeated measures with a single factor. The results indicated that the TAC and SOD were considerably higher in T1, compared to that in T0, T1, and T2. In addition, CAT was considerably higher in T2 than in T0, T1, and T3. However, ROS and MDA did not differ significantly among the experimental groups. Despite the absence of a statistically significant difference among experimental groups, MDA decreased quantitatively on T1, relative to other experimental groups. In conclusion, a deficiency in vitamin D3 has a potential antioxidant capability, introducing a novel method for extending sperm storage.
کلیدواژه‌ها
Chilled semen؛ Awassi ram؛ Vitamin D3؛ Antioxidant
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مراجع
  1. Soltanpour F, Moghaddam G. Effects of frozen diluents on storage of ram sperm. Int J Adv Biologic Biomed Res. 2013;1(12):1698-704.
  2. Verberckmoes S, Van Soom A, Dewulf J, de Kruif A. Comparison of three diluents for the storage of fresh bovine semen. Theriogenology. 2005;63(3):912-22.
  3. Falchi L, Galleri G, Zedda MT, Pau S, Bogliolo L, Ariu F, et al. Liquid storage of ram semen for 96h: Effects on kinematic parameters, membranes and DNA integrity, and ROS production. Livestock Sci. 2018;207:1-6.
  1. Maxwell WM, Salamon S. Liquid storage of ram semen: a review. Reprod Fertil Dev. 1993;5(6):613-38.
  2. Salamon S, Maxwell WM. Storage of ram semen. Anim Reprod Sci. 2000;62(1-3):77-111.
  3. Sanocka D, Kurpisz M. Reactive oxygen species and sperm cells. Reprod Biol Endocrinol. 2004;2:12.
  4. Aitken RJ, Baker MA. Oxidative stress, sperm survival and fertility control. Mol Cell Endocrinol. 2006;250(1-2):66-9.
  5. Koppers AJ, De Iuliis GN, Finnie JM, McLaughlin EA, Aitken RJ. Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J Clin Endocrinol Metab. 2008;93(8):3199-207.
  6. Ray PD, Huang BW, Tsuji Y. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal. 2012;24(5):981-90.
  7. Kameni SL, Meutchieye F, Ngoula F. Liquid storage of ram semen: Associated damages and improvement. Open J Anim Sci. 2021;11(3):473-500.
  8. Gundogan M, Yeni D, Avdatek F, Fidan AF. Influence of sperm concentration on the motility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage. Anim Reprod Sci. 2010;122(3-4):200-7.
  9. Maxwell WMC, Watson PF. Recent progress in the preservation of ram semen. Anim Reprod Sci. 1996;42(1):55-65.
  10. Wagner H, Cheng JW, Ko EY. Role of reactive oxygen species in male infertility: An updated review of literature. Arab J Urol. 2018;16(1):35-43.
  11. Wiseman H. Vitamin D is a membrane antioxidant. Ability to inhibit iron-dependent lipid peroxidation in liposomes compared to cholesterol, ergosterol and tamoxifen and relevance to anticancer action. FEBS Lett. 1993;326(1-3):285-8.
  12. Bresm MH, Habeeb HM. Effect of Adding Different Vitamin D3 Concentrations on Chilled Storage of Awassi Ram Semen. InIOP Conference Series: Earth and Environmental Science. 2022;1060(1): 012084.
  13. Rehman R, Lalani S, Baig M, Nizami I, Rana Z, Gazzaz ZJ. Association Between Vitamin D, Reproductive Hormones and Sperm Parameters in Infertile Male Subjects. Front Endocrinol (Lausanne). 2018;9:607.
  14. Asadpour R, Taravat M, Rahbar M, Khoshniyat M, Hamidian G. Effects of vitamin D supplementation in extender on sperm kinematics and apoptosis following the freeze-thaw process in normozoospermic and asthenozoospermic Holstein bulls. Basic Clin Androl. 2021;31(1):20.
  15. Maghsoumi-Norouzabad L, Zare Javid A, Mansoori A, Dadfar M, Serajian A. Evaluation of the effect of vitamin D supplementation on spermatogram, seminal and serum levels of oxidative stress indices in asthenospermia infertile men: a study protocol for a triple-blind, randomized controlled trial. Nutr J. 2021;20(1):49.
  16. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978;52:302-10.
  17. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38(12):1103-11.
  18. Apak R, Guclu K, Demirata B, Ozyurek M, Celik SE, Bektasoglu B, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules. 2007;12(7):1496-547.
  19. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47(3):469-74.
  20. Goth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta. 1991;196(2-3):143-51.
  21. SAS. Statistical Analysis System, User's Guide Inst. Inc. Cary. NC. USA: SAS; 2012.
  22. Duncan DB. Multiple range and multiple F tests. Biometrics. 1955;11(1):1-42.
  23. Khalifa MA. International Journal of Animal Research (ISSN: 2575-7822) Effect of supplementing ram semen extender with melatonin on oxidative stress indices and physical properties of chilled spermatozoa. J Anim Res. 2017;1:14.
  24. Wu C, Dai J, Zhang S, Sun L, Liu Y, Zhang D. Effect of Thawing Rates and Antioxidants on Semen Cryopreservation in Hu Sheep. Biopreserv Biobank. 2021;19(3):204-9.
  25. Dada R, Bisht S. Oxidative Stress and Male Infertility. In: Singh R, Singh K, editors. Male Infertility: Understanding, Causes and Treatment. Singapore: Springer Singapore; 2017. p. 151-65.
  26. Hsieh YY, Chang CC, Lin CS. Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility. Int J Biol Sci. 2006;2(1):23-9.
  27. Pesch S, Bergmann M, Bostedt H. Determination of some enzymes and macro- and microelements in stallion seminal plasma and their correlations to semen quality. Theriogenology. 2006;66(2):307-13.
  28. Jeulin C, Soufir JC, Weber P, Laval-Martin D, Calvayrac R. Catalase activity in human spermatozoa and seminal plasma. Gamete Res. 1989;24(2):185-96.
  29. Câmara DR, Mello-Pinto MMC, Pinto LC, Brasil OO, Nunes JF, Guerra MMP. Effects of reduced glutathione and catalase on the kinematics and membrane functionality of sperm during liquid storage of ram semen. Small Rumin Res. 2011;100(1):44-9.
  30. Chen H, Chow PH, Cheng SK, Cheung AL, Cheng LY, O WS. Male genital tract antioxidant enzymes: their source, function in the female, and ability to preserve sperm DNA integrity in the golden hamster. J Androl. 2003;24(5):704-11.
  1. Camara DR, Silva SV, Almeida FC, Nunes JF, Guerra MM. Effects of antioxidants and duration of pre-freezing equilibration on frozen-thawed ram semen. Theriogenology. 2011;76(2):342-50.
  2. Maxwell WM, Stojanov T. Liquid storage of ram semen in the absence or presence of some antioxidants. Reprod Fertil Dev. 1996;8(6):1013-20.
  3. Tavilani H, Goodarzi MT, Doosti M, Vaisi-Raygani A, Hassanzadeh T, Salimi S, et al. Relationship between seminal antioxidant enzymes and the phospholipid and fatty acid composition of spermatozoa. Reprod Biomed Online. 2008;16(5):649-56.
  4. Zhong W, Gu B, Gu Y, Groome LJ, Sun J, Wang Y. Activation of vitamin D receptor promotes VEGF and CuZn-SOD expression in endothelial cells. J Steroid Biochem Mol Biol. 2014;140:56-62.
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