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Rafiee, Dana, Ebrahimi, Mohammad Ali, Qavami, Nassrin, Zarinpanjeh, Nasim. (1402). The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi. سامانه مدیریت نشریات علمی, 12(4), 397-404. doi: 10.22092/jmpb.2022.358302.1465
Dana Rafiee; Mohammad Ali Ebrahimi; Nassrin Qavami; Nasim Zarinpanjeh. "The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi". سامانه مدیریت نشریات علمی, 12, 4, 1402, 397-404. doi: 10.22092/jmpb.2022.358302.1465
Rafiee, Dana, Ebrahimi, Mohammad Ali, Qavami, Nassrin, Zarinpanjeh, Nasim. (1402). 'The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi', سامانه مدیریت نشریات علمی, 12(4), pp. 397-404. doi: 10.22092/jmpb.2022.358302.1465
Rafiee, Dana, Ebrahimi, Mohammad Ali, Qavami, Nassrin, Zarinpanjeh, Nasim. The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi. سامانه مدیریت نشریات علمی, 1402; 12(4): 397-404. doi: 10.22092/jmpb.2022.358302.1465

The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi

Journal of Medicinal plants and By-products
مقاله 10، دوره 12، شماره 4، اسفند 2023، صفحه 397-404    اصل مقاله (1.21 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2022.358302.1465
نویسندگان
Dana Rafiee1؛ Mohammad Ali Ebrahimi1؛ Nassrin Qavami2؛ Nasim Zarinpanjeh* 2
1Department of Agricultural Biotechnology, Payam Noor University, Tehran, Iran
2Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
چکیده
Nitraria schoberi L. is a medicinal plant with antioxidant, antifungal, anti-inflammatory and, anti-viral properties. This plant is also tolerant to salinity and drought. The main aim of this study was to perform elicitation in cell suspension culture of N. schoberi to detect the production of total phenolic and flavonoid contents. In order to accomplish this study, hypocotyls and cotyledonary leaves of N. schoberi were cultured in different callus induction treatments. At the next step, the calli from the best callus induction treatment were transferred to cell suspension cultures containing sodium chloride (NaCl) and salicylic acid (SA) alone or in combination. Total phenolic and flavonoid contents in treatments were measured using a spectrophotometer (absorbance at 765 nm and 430 nm, respectively). According to the results, although callus induction was observed in all treatments, the maximum fresh weight (FW) (7.6 g) and dry weight (DW) (3.7 g) were obtained by culturing hypocotyl explants in MS medium supplemented with 2, 4-dichrolophenoxyacetic acid (2, 4-D) at 0.5 mg/L. The highest total phenolic content (62.2 mg/g) and total flavonoid content (48.24 mg/g) were detected in suspension culture containing 100 millimolar (mM) NaCl and 50 micromolar (µM) SA. Elicitation in suspension culture of N. schoberi can be an effective method for increasing the production of secondary metabolites (phenolic and flavonoid compounds).
کلیدواژه‌ها
Callus induction؛ Elicitor؛ Medicinal plants؛ Secondary metabolites؛ Suspension culture
مراجع
 

  1. Narayani M., Srivastava S. Elicitation: a stimulation of stress in in vitro plant cell/tissue cultures for enhancement of secondary metabolite production. Phytochem Rev. 2017; 16: 1227-1252.
  2. Tabassam S., Anwar M.A., Gulfraz M., Arshad M., Sabitaliyevich U.Y., Nurmurzayevich S.B., Ahmad M.S.
  3. Bioactivity evaluation and HPLC UV-VIS based quantification of antioxidant secondary metabolites from extract and fractions of Bistorta amplexicaulis rhizome. Cellular and Molecular Biology. 2019; 65: 19-26.
  4. Cecilia M.K., Abrha A.H., Hlengilizwe N., Hintsa A.T., Umer M., Nixwell M.F., Ali S.W., Afzal M.I., Martorell M., Salehi B., Setzer W.N., Sattar M.U., Imran M., Sharifi-Rad J. Untargeted profiling of field cultivated bush tea (Athrixia phylicoides DC.) based on metabolite analysis. Cellular and Molecular Biology. 2020; 66: 104-109.
  5. Salehi B., Tsouh Fokou P.V., Yamthe L.R.T., Tali B.T., Adetunji C.O., Rahavian A., Mudau F.N., Martins N., Martorell M., Rodrigues C.F., Setzer W.N., Sharifi-Rad M., Amarowicz R., Sharifi-Rad J. Insights on the anticancer potential of plant-food bioactives: A key focus to prostate cancer. Cellular and Molecular Biology. 2020; 66: 250-263.
  6. Salehi B., Selamoglu Z., Sevindik M., Fahmy N.M., Al-Sayed E., El-Shazly M., Csupor-Löffler B., Csupor D., Yazdi SE, Sharifi-Rad J, Arserim-Uçar DK, Arserim EH, Karazhan N, Jahani A, Dey A, Azadi H, Vakili SA, Sharopov F, Martins N., Büsselberg D. Achillea spp.: A comprehensive review on its ethnobotany, phytochemistry, phytopharmacology and industrial applications. Cellular and Molecular Biology. 2020; 66: 78-103.
  7. Alavi M., Adulrahman N.A., Haleem A.A., Al-Râwanduzi A.D.H., Khusro A., Abdelgawad M.A., Ghoneim M.M., Batiha G.E.S., Kahrizi D., Martinez F., Koirala N. Nanoformulations of curcumin and quercetin with silver nanoparticles for inactivation of bacteria. Cellular and Molecular Biology. 2022; 67:151-156.
  8. Sharifi-Rad J., Hoseini-Alfatemi SM, Sharifi-Rad M and Teixeira da Silva JA. Antibacterial, antioxidant, antifungal and anti-inflammatory activities of crude extract from Nitraria schoberi fruits. 3 Biotech. 2015; 5: 677-684.
  9. Suo Y., Wang L. Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification. Eur J Lipid Sci Technol. 2010; 112: 390-399.
  10. Hadj Salem J., Chevalot I., Harscoat-Schiavo C., Paris C., Fick M., Humeau C. Biological activities of flavonoids from Nitraria retusa (Forssk.) Asch. and their acylated derivatives. Food Chem. 2011; 124: 486-494.
  11. Khajeddini M.A., Dadpour M.R., Khodaverdi M., Naghiloo S. The GC-MS analyses of the n-hexane extract of Nitraria schoberi L., its total phenolics and in vitro antioxidant activity. J. Med. Plants Res. 2012; 6: 4874- 4878.
  12. Zheleznichenko T., Banaev E., Asbaganov S., Voronkova M., Kukushkina T., Filippova E., Mazurkova N., Shishkina L., Novikova T., Nitraria schoberi L. hairy root culture as a source of compounds with antiviral activity against influenza virus subtypes А(H5N1) and А(H3N2). 3 Biotech. 2018; 8: 260.
  13. Chan L.K., Lim P.S., Choo M.L., Boey P.L. Establishment of Cyperus aromaticus cell suspension cultures for the production of Juvenile hormone III. In vitro Cell Dev Biol Plant. 2010; 46: 8-12.
  14. Rajan M., Feba K.S., Chandran V., Shahena S., Mathew L. Enhancement of rhamnetin production in Vernonia anthelmintica (L.) Willd. Cell suspension cultures by eliciting with methyl jasmonate and salicylic acid. Physiol Mol Biol Plants. 2020; doi: 10.1007/s12298-020-00829-8.
  15. Dastmalchi T., Omidi M., Azizinezhad R., Rezazadeh S., Etminan A. Effects of methyl jasmonate and phloroglucinol on thebaine and sanguinarine production in cell suspension culture of Persian poppy (Papaver bracteatum Lindl.). Cellular and Molecular Biology. 2019; 65: 11-17.
  16. Leroux M.M., Doumandji Z., Chezeau L., Hocquel R., Ferrari L., Joubert O., Rihn P., Rihn BH.Validation of an air/liquid interface device for TiO2 nanoparticle toxicity assessment on NR8383 cells: preliminary results. Cellular and Molecular Biology. 2020; 66:112-116.
  17. Kolewe M.E., Gaurav V., Roberts S.C. Pharmaceutically active natural product synthesis and supply via plant cell culture technology. Mol Pharm. 2008; 5: 243-256.
  18. Boonsnongcheep P., Korsangruang S., Soonthornchareonnon N., Chintapakorn Y., Saralamp P., Prathanturarug S. Growth and isoflavonoid accumulation of Pueraria candollei var. Candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell Tiss Organ Cult. 2010; 101: 119-126.
  19. Bonfill M., Mangas S., Moyano E., Cusido R.M., Palazo´n J. Production of centellosides and phytosterols in cell suspension cultures of Centella asiatica. Plant Cell Tiss Organ Cult. 2011; 104: 61-67.
  20. Deepthi S., Satheeshkumar K. Enhanced camptothecin production induced by elicitors in the cell suspension cultures of Ophiorrhiza mungos Linn. Plant Cell Tiss Organ Cult. 2016; 124: 483-493.
  21. Zhao J.L., Zhou L.G., Wu J.Y. Effects of biotic and abiotic elicitors on cell growth and tanshinone accumulation in Salvia miltiorrhiza cell cultures. Appl Microbiol Biot. 2010; 87: 137-144.
  22. Koul A., Mallubhotla S. Elicitation and enhancement of bacoside production using suspension cultures of Bacopa monnieri (L.) Wettst. 3 Biotech. 2020; 10: 256.
  23. Ramirez-Estrada K., Vidal-Limon H., Hidalgo D., Moyano E., Golenioswki M., Cusidó R.M., Palazon J. Elicitation, an Effective Strategy for the Biotechnological Production of Bioactive High-Added Value Compounds in Plant Cell Factories. Molecules. 2016; 21: 182.
  24. Mendozaa D., Cuaspuda O., Ariasa J.P., Ruizc O., Ariasa M. Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana. Biotech. Reports. 2018; 19:1-9.
  25. Gupta P., Sharma S., Saxena S. Effect of Salts (NaCl and Na2CO3) on Callus and Suspension Culture of Stevia rebaudiana for Steviol glycoside Production. Appl. Biochem. Biotechnol. 2014; doi: 10.1007/s12010-014-0736-2.
  26. Deshmukh V., Deshpande J., Wani M. In vitro Enhancement of Solasodine Production by Using Biotic and Abiotic Elicitors in Different Solanum Spp. Annals of R.S.C.B. 2021; 25: 18195-18207.
  27. Kheirabadi S., Zarinpanjeh N., Ebrahimi M.A., Bakhshi Khaniki G.R., Naser H.R. Effective in vitro seed germination and direct regeneration from cotyledonary leaf explants of Nitraria schoberi. Iranian Journal of Genetic and Plant breeding. 2020; 9: 10-16.
  28. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 1962; 15: 473-497.
  29. Amirkavei Najafabadi B., Qavami N., Ebrahimi M.A., Ebrahimi P., Zarinpanjeh N. Enhancement of Taxol production by applying amino acid complex along with chitosan in suspension culture of Taxus baccata L. J. Med. Plants. 2020; 19(76): 99-109.
  30. Ali B. Salicylic acid: An efficient elicitor of secondary metabolite production in plants. Biocatalysis and Agricultural Biotechnology. 2021; doi:10.1016/j.bcab.2020.101884.
  31. Hemmati N., Cheniany M., Ganjeali A. Effect of plant growth regulators and explants on callus induction and study of antioxidant potentials and phenolic metabolites in Salvia tebesana Bunge. Botanica serbica. 2020; 44: 163-173.
  32. Sharma G., Nautiyal A.R. Influence of explants type and plant growth regulators on in vitro multiple shoots regeneration of a laurel from Himalaya. Nature and Science. 2009; 7: 1-7.
  33. Yang Y., Wei X., Shi R., Fan Q., An L.Salinity-induced Physiological Modification in the Callus from Halophyte Nitraria tangutorum Bobr. J Plant Growth Regul. 2010; 29:465-476.
  34. Mei X., Shen Y. Nitraria sibirica cell suspension culture: establishment, characterization and application. J. For. Res. 2017. doi: 10.1007/s11676-017-0367-x.
  35. Adil M., Ren X., Kang D., The Thi L., Jeong B.R. Effect of explant type and plant growth regulators on callus induction, growth and secondary metabolites production in Cnidium officinale Makino. Molecular Biology Reports. 2018; 45: 1919-1927.
  36. Kang S.M., Min J.Y., Kim Y.D., Karigar C.S., Kim S.W., Goo GH., Choi M.S. Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures. J Biotechnol. 2009; 139: 84- 88.
  37. Cai Z., Riedel H., Saw N.M.M.T., Mewis I., Reineke K., Knorr D., Smetanska I. Effects of elicitors and high hydrostatic pressure on secondary metabolism of Vitis vinifera suspension culture. Process Biochem. 2011; 46: 1411-1416.
  38. Srivastava S., Srivastava A.K. Effect of elicitors and precursors on azadirachtin production in hairy root culture of Azadirachta indica. Appl Biochem Biotechnol. 2013; 172: 2286- 2297.
  39. Moharramnejad S., Azam A.T., Panahandeh J., Dehghanian Z., Ashraf M. Effect of methyl jasmonate and salicylic acid on in vitro growth, stevioside production, and oxidative defense system in Stevia rebaudiana. Sugar Tech. 2019; 21: 1031-1038.
  40. Khan T., Khan T., Hano C., Abbasi B.H. Effects of chitosan and salicylic acid on the production of pharmacologically attractive secondary metabolites in callus cultures of Fagonia indica Ind. Crops Prod. 2019; 129: 525-535.
  41. Rezaei A., Ghanati F., Behmanesh M., Mokhtari-dizaji M. Ultrasound-potentiated salicylic acid-induced physiology effects and production of taxol in Hazelnut (Corylus avellana L.). Ultrasound Med. Biol. 2011; 37: 1938-1947.
  42. Karalija E., Zeljković S.C., Parić A. Harvest time–related changes in biomass, phenolics and antioxidant potential in Knautia sarajevensis shoot cultures after elicitation with salicylic acid and yeast. In Vitro Cell. Dev. Biol. Plant. 2019; 56: 177-183.
  43. Chung I.M., Rekha K., Rajakumar G., Thiruvengadam M. Jasmonic acid and saliculic acid enhanced phytochemical production and biological activities in cell suspension cultures of spine gourd (Momordica dioica Roxb). Acta Biol. Hung. 2017; 68: 88-100.
  44. Golkar P., Taghizadeh M., Yousefian Z. The effects of chitosan and salicylic acid on elicitation of secondary metabolites and antioxidant activity of safflower under in vitro salinity stress. Plant Cell Tiss. Organ Cult. 2019; 137: 575-585.
  45. Chavoushi M., Najafi F., Salimi A., Angaji S.A. Effect of salicylic acid and sodium nitroprusside on growth parameters, photosynthetic pigments and secondary metabolites of safflower under drought stress. Sci. Hortic. 2020; 259: 108823.
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