اخبار و اعلانات

 آمار

تعداد نشریات283
تعداد نشریات سازمان81
تعداد شماره‌ها2,726
تعداد مقالات31,042
تعداد مشاهده مقاله27,939,500
تعداد دریافت فایل اصل مقاله16,238,227
Ali Albadery, A, Shakir Mahdi Al-Amara, S, Abd-Al-Ridha Al-Abdullah, A. (1402). Phenotyping and Genotyping Evaluation of E. coli Produces Carbapenemase Isolated from Cancer Patients in Al-Basrah, Iraq. سامانه مدیریت نشریات علمی, 78(3), 823-829. doi: 10.22092/ari.2022.359869.2493
A Ali Albadery; S Shakir Mahdi Al-Amara; A Abd-Al-Ridha Al-Abdullah. "Phenotyping and Genotyping Evaluation of E. coli Produces Carbapenemase Isolated from Cancer Patients in Al-Basrah, Iraq". سامانه مدیریت نشریات علمی, 78, 3, 1402, 823-829. doi: 10.22092/ari.2022.359869.2493
Ali Albadery, A, Shakir Mahdi Al-Amara, S, Abd-Al-Ridha Al-Abdullah, A. (1402). 'Phenotyping and Genotyping Evaluation of E. coli Produces Carbapenemase Isolated from Cancer Patients in Al-Basrah, Iraq', سامانه مدیریت نشریات علمی, 78(3), pp. 823-829. doi: 10.22092/ari.2022.359869.2493
Ali Albadery, A, Shakir Mahdi Al-Amara, S, Abd-Al-Ridha Al-Abdullah, A. Phenotyping and Genotyping Evaluation of E. coli Produces Carbapenemase Isolated from Cancer Patients in Al-Basrah, Iraq. سامانه مدیریت نشریات علمی, 1402; 78(3): 823-829. doi: 10.22092/ari.2022.359869.2493

Phenotyping and Genotyping Evaluation of E. coli Produces Carbapenemase Isolated from Cancer Patients in Al-Basrah, Iraq

Archives of Razi Institute
مقاله 7، دوره 78، شماره 3، مرداد و شهریور 2023، صفحه 823-829    اصل مقاله (635.23 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359869.2493
نویسندگان
A Ali Albadery* 1؛ S Shakir Mahdi Al-Amara1؛ A Abd-Al-Ridha Al-Abdullah2
1Department of Biology, College of Science, University of Basrah, Basrah, Iraq
2Department of Pathological analyses, College of Science, University of Basrah, Basrah, Iraq
چکیده
One of the most important nosocomial organisms that cause urinary tract infections (UTIs) in cancer patients is Escherichia coli. A significant cause of concern in managing UTIs is the development of carbapenem-resistant bacteria. Escherichia coli with carbapenem resistance has become a more serious problem, particularly in Iraq. In this regard, the present study aimed to estimate the prevalence of carbapenem-resistant E. coli in Al-Basrah, Iraq. Conventional tests and the Vitek®2 system were used to identify the isolates and determine the susceptibility of E.coli isolates to antimicrobials. In addition, E.coli isolates were tested by mCIM and eCIM methods. Moreover, the major carbapenemase genes, including blaSPM, blaIMP, blaVIM, and blaKPC were detected by polymerase chain reaction. In total, 120 urine samples were collected from cancer patients who were suspected of having urinary tract infections at Basrah Center of Oncology Al-Sader Teaching Hospital, Basrah, Iraq. Identification of bacterial growth by using biochemical tests revealed different bacterial species. The most frequent bacteria were E. coli (n=22, 53.65%) isolates. The results showed that 13 (59.09%) and 11 (50%) out of 22 E. coli isolates were positive for the production of carbapenemase, based on the eCIM and sCIM, respectively. The majority of E.coli in this study possessed the blaVIM gene (n=13, 59.1%), followed by the blaKPC gene (n=5, 22.73%), blaIMP gene (n=5, 22.73%), and blaSPM gene (n=4, 18.18%). There is a spread of more than one type of carbapenemase among the E. coli isolates collected from UTI cancer patients in Basrah Hospital. The E. coli identified in the current study had a strong capacity to produce carbapenemase enzymes against the four generations of antibiotics, including imipenem and meropenem antibiotics.
کلیدواژه‌ها
Cancer؛ Carbapenemase؛ E.coli؛ Urinary tract infections
سایر فایل های مرتبط با مقاله
مراجع
  1. Mahmoud AT, Salim MT, Ibrahem RA, Gabr A, Halby HM. Multiple drug resistance patterns in various phylogenetic groups of hospital-acquired uropathogenic E. coli isolated from cancer patients. Antibiotics. 2020;9(3):108.
  2. Tigabu A, Ferede W, Belay G, Gelaw B. Prevalence of asymptomatic bacteriuria and antibiotic susceptibility patterns of bacterial isolates among cancer patients and healthy blood donors at the University of Gondar Specialized Hospital. Int J Microbiol. 2020;2020.
  3. Flores-Mireles A, Hreha TN, Hunstad DA. Pathophysiology, treatment, and prevention of catheter-associated urinary tract infection. Topics in spinal cord injury rehabilitation. 2019;25(3):228-40.
  4. Abd El Ghany M, Sharaf H, Al-Agamy MH, Shibl A, Hill-Cawthorne GA, Hong P-Y. Genomic characterization of NDM-1 and 5, and OXA-181 carbapenemases in uropathogenic Escherichia coli isolates from Riyadh, Saudi Arabia. PloS One. 2018;13(8):e0201613.
  5. Korbel L, Howell M, Spencer JD. The clinical diagnosis and management of urinary tract infections in children and adolescents. Paediatr Int Child Health. 2017;37(4):273-9.
  6. Cooper AL, Carter C, McLeod H, Wright M, Sritharan P, Tamber S, et al. Detection of carbapenem-resistance genes in bacteria isolated from wastewater in Ontario. FACETS. 2021;6(1):569-91.
  7. Li J, Li C, Cai X, Shi J, Feng L, Tang K, et al. Performance of modified carbapenem inactivation method and inhibitor-based combined disk test in the detection and distinguishing of carbapenemase producing Enterobacteriaceae. Ann Transl Med. 2019;7(20).
  8. Elbadawi HS, Elhag KM, Mahgoub E, Altayb HN, Ntoumi F, Elton L, et al. Detection and characterization of carbapenem resistant Gram‐negative bacilli isolates recovered from hospitalized patients at Soba University Hospital, Sudan. BMC Microbiol. 2021;21(1):1-9.
  9. Forbes BA, Sahm DF, Weissfeld AS. Diagnostic microbiology: Mosby St Louis; 2007.
  10. Prescott LM, Harley JP. Harley Prescott: Laboratory Exercises in Microbiology, Fifth Editio. The McGraw− Hill; 2002.
  11. CLSI CLSI. Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute Wayne, PA; 2017.
  12. Sfeir M, Hayden J, Fauntleroy K, Mazur C, Johnson J, Simner P, et al. EDTA-modified carbapenem inactivation method: a phenotypic method for detecting metallo-β-lactamase-producing Enterobacteriaceae. J Clin Microbiol. 2019;57(5):e01757-18.
  13. Shoja S, Moosavian M, Peymani A, Tabatabaiefar MA, Rostami S, Ebrahimi N. Genotyping of carbapenem resistant Acinetobacter baumannii isolated from tracheal tube discharge of hospitalized patients in intensive care units, Ahvaz, Iran. Iran J Microbiol. 2013;5(4):315.
  14. Mushi MF, Mshana SE, Imirzalioglu C, Bwanga F. Carbapenemase genes among multidrug resistant gram negative clinical isolates from a tertiary hospital in Mwanza, Tanzania. BioMed Res Int. 2014;2014.
  15. Mahmoud NE, Altayb HN, Gurashi RM. Detection of carbapenem-resistant genes in escherichia coli Isolated from drinking water in Khartoum, Sudan. J Environ Public Health.. 2020;2020.
  16. Ogefere HO, Omoregie R, Iriah SE. Urinary tract infection among cancer patients in Benin City, Nigeria. Int J Biomed Health Sci. 2021;7(3).
  17. Munyi S, Macharia W, Alwar A, Njeru EK. Screening for urinary tract infection in children with cancer. East African medical journal. 1998;75(5):264-7.
  18. Al-naimi IA, Abbas MA. Research article recurrent urinary tract infections in iraqi leukemic patients. Int J. 2016;4(3):1433-9.
  19. Shrestha G, Wei X, Hann K, Soe KT, Satyanarayana S, Siwakoti B, et al. Bacterial profile and antibiotic resistance among cancer patients with urinary tract infection in a national tertiary cancer hospital of Nepal. Trop Med Infect Dis. 2021;6(2):49.
  20. Islam MR, Akhter F, Khatun N, Rahman M. Bacterial Causes of Infections and Their Antimicrobial Sensitivity Pattern in Cancer Patients. 2021.
  21. Pierce VM, Simner PJ, Lonsway DR, Roe-Carpenter DE, Johnson JK, Brasso WB, et al. Modified carbapenem inactivation method for phenotypic detection of carbapenemase production among Enterobacteriaceae. J Clin Microbiol. 2017;55(8):2321-33.
  22. Jing X, Zhou H, Min X, Zhang X, Yang Q, Du S, et al. The simplified carbapenem inactivation method (sCIM) for simple and accurate detection of carbapenemase-producing gram-negative bacilli. Front Microbiol. 2018;9:2391.
  23. Freire M, Pierrotti L, Ibrahim K, Magri A, Bonazzi P, Hajar L, et al. Infection with Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae in cancer patients. Eur J Clin Microbiol Infect Dis. 2015;34(2):277-86.
  24. van Duin D, Doi Y. The global epidemiology of carbapenemase-producing Enterobacteriaceae. Virulence. 2017;8(4):460-9.
  25. Carugati M, Piazza A, Peri A, Cariani L, Brilli M, Girelli D, et al. Fatal respiratory infection due to ST308 VIM-1-producing Pseudomonas aeruginosa in a lung transplant recipient: case report and review of the literature. BMC Infect Dis. 2020;20(1):1-7.
  26. Hishinuma T, Uchida H, Tohya M, Shimojima M, Tada T, Kirikae T. Emergence and spread of VIM-type metallo-β-lactamase-producing Pseudomonas aeruginosa clinical isolates in Japan. J Glob Antimicrob Resist. 2020;23:265-8.
  27. Lai K, Ma Y, Guo L, An J, Ye L, Yang J. Molecular characterization of clinical IMP-producing Klebsiella pneumoniae isolates from a Chinese Tertiary Hospital. Ann Clin Microbiol Antimicrob. 2017;16(1):1-5.
  28. Chaves L, Tomich LM, Salomão M, Leite GC, Ramos J, Martins RR, et al. High mortality of bloodstream infection outbreak caused by carbapenem-resistant P. aeruginosa producing SPM-1 in a bone marrow transplant unit. J Med Microbiol. 2017;66(12):1722-9.
  29. Nascimento AP, Ortiz MF, Martins WM, Morais GL, Fehlberg LC, Almeida LG, et al. Intraclonal genome stability of the metallo-β-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an endemic clone disseminated in Brazilian hospitals. Front Microbiol. 2016;7:1946.
  30. Ali SB, Perdawood D, Abdulrahman R, Al Farraj DA, Alkubaisi NA. Vitamin D deficiency as a risk factor for urinary tract infection in women at reproductive age. Saudi Journal of Biological Sciences. 2020;27(11):2942-7.
  31. Bonardi S, Pitino R. Carbapenemase-producing bacteria in food-producing animals, wildlife and environment: A challenge for human health. Ital J Food Saf. 2019;8(2).
  32. Al-Hasso M. Molecular detection of carbapenemases in Gram negative bacilli isolated from different sources in Mosul city, Iraq. Kuwait J Sci. 2021.
  33. Le Terrier C, Masseron A, Uwaezuoke NS, Edwin CP, Ekuma AE, Olugbeminiyi F, et al. Wide spread of carbapenemase-producing bacterial isolates in a Nigerian environment. J Glob Antimicrob Resist. 2020;21:321-3.
  34. Hamza E, Dorgham SM, Hamza DA. Carbapenemase-producing Klebsiella pneumoniae in broiler poultry farming in Egypt. J Glob Antimicrob Resist. 2016;7:8-10.
آمار
تعداد مشاهده مقاله: 1,083
تعداد دریافت فایل اصل مقاله: 275


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب