اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,083
تعداد مقالات34,394
تعداد مشاهده مقاله48,233,096
تعداد دریافت فایل اصل مقاله78,996,447
Waheed Khudiar Khareeb, Z, Ismail Ibrahim, Z, Ali Abdullah, F. (1401). Pathological and Molecular detection of Mycoplasma ovipneumoneae in Sheep, Basrah Province, Iraq. سامانه مدیریت نشریات علمی, 77(6), 2073-2080. doi: 10.22092/ari.2022.357996.2134
Z Waheed Khudiar Khareeb; Z Ismail Ibrahim; F Ali Abdullah. "Pathological and Molecular detection of Mycoplasma ovipneumoneae in Sheep, Basrah Province, Iraq". سامانه مدیریت نشریات علمی, 77, 6, 1401, 2073-2080. doi: 10.22092/ari.2022.357996.2134
Waheed Khudiar Khareeb, Z, Ismail Ibrahim, Z, Ali Abdullah, F. (1401). 'Pathological and Molecular detection of Mycoplasma ovipneumoneae in Sheep, Basrah Province, Iraq', سامانه مدیریت نشریات علمی, 77(6), pp. 2073-2080. doi: 10.22092/ari.2022.357996.2134
Waheed Khudiar Khareeb, Z, Ismail Ibrahim, Z, Ali Abdullah, F. Pathological and Molecular detection of Mycoplasma ovipneumoneae in Sheep, Basrah Province, Iraq. سامانه مدیریت نشریات علمی, 1401; 77(6): 2073-2080. doi: 10.22092/ari.2022.357996.2134

Pathological and Molecular detection of Mycoplasma ovipneumoneae in Sheep, Basrah Province, Iraq

Archives of Razi Institute
مقاله 8، دوره 77، شماره 6، بهمن و اسفند 2022، صفحه 2073-2080    اصل مقاله (835.3 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357996.2134
نویسندگان
Z Waheed Khudiar Khareeb* 1؛ Z Ismail Ibrahim2؛ F Ali Abdullah3
1Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Basrah, Basrah, Iraq
2Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
3Department of Microbiology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq
چکیده
Mycoplasma ovipneumonea (M. ovipneumonea) are microorganism's causes atypical pneumonia in (sheep and goat). Mycoplasma is isolated frequently from pneumonic cases (lung, trachea, and nose) of sheep but can also be found in the respiratory tract of healthy sheep. This study aimed to isolate, identify, and pathological examination of M. ovipneumonea in sheep. Samples in the current study were collected from sheep of both sex and 6-10 months of age in Basrah slaughterhouse, suffering from respiratory signs associated with ocular, nasal discharge, and coughing. Nasal swabs were collected from the nose before slaughtering; other swabs were collected from the trachea and bifurcation of bronchus for bacterial isolation on PPLOs. Tissue specimens are frozen for DNA gene-based PCR analysis and for preparing paraffin blocks for histopathological examination. The bacterial cultures revealed isolates of Mycoplasma were positive on (PPLO) broth with agar from the morphological colonies of Mycoplasmaovipeumonea "fried egg" type colony morphology. PCR results revealed the 16S rRNA gene of Mycoplasma sp. The appearance revealed different stages of pulmonary changes like respiratory congestion, edema, and hemorrhagic spots on the surface of the lungs, and their air passages contained inflammatory exudate. The microscopic lesions represent acute fibrinous-suppurative broncho-interstitial pneumonia. M. ovipneumoniae was a prevalent respiratory infectious disease in Iraqi's sheep-Basrah province with frequent bacterial isolation, pneumonic pathological changes in animals suffer from different respiratory manifestations.
کلیدواژه‌ها
Mycoplasma ovipneumonia؛ pathology؛ PCR؛ sheep
سایر فایل های مرتبط با مقاله
مراجع
  1. Di Provvido A, Averaimo D, Zilli K, Marruchella G, Scacchia M. Mycoplasma pneumonia in small ruminants: A ten-year long retrospective survey. Small Rumin Res. 2017;153:103-6.
  2. Walsh DP, Cassirer EF, Bonds MD, Brown DR, Edwards WH, Weiser GC, et al. Concordance in diagnostic testing for respiratory pathogens of bighorn sheep. Wildl Soc Bull. 2016;40(4):634-42.
  3. Daee AA, Khodakaram-Tafti A, Derakhshandeh A, Seyedin M. Identification of Mycoplasma ovipneumoniae and Mycoplasma arginini in sheep with pneumonia in North East of Iran. Iran J Vet Res. 2020;21(1):15-9.
  4. Dassanayake RP, Shanthalingam S, Herndon CN, Subramaniam R, Lawrence PK, Bavananthasivam J, et al. Mycoplasma ovipneumoniae can predispose bighorn sheep to fatal Mannheimia haemolytica pneumonia. Vet Microbiol. 2010;145(3-4):354-9.
  5. Jiang Z, Song F, Li Y, Xue D, Deng G, Li M, et al. Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells. Mediators Inflamm. 2017;2017:9891673.
  6. Zhu M, Cao S, Zheng W, Zhai M, Wang M, Blair HT, et al. miR-509-5p anti-infection response for mycoplasma pneumonia in sheep by targeting NF-kappaB pathway. Vet Immunol Immunopathol. 2021;238:110275.
  7. Finkbeiner W, Ursell P, Davis R. Autopsypatholoy: Manual and atlas: Saunders an imprint of Elsevier Inc; 2009.
  8. Luna L. manual of histological. 3rd ed: Grow- Hill book; 1968.
  9. Einarsdottir T, Gunnarsson E, Hjartardottir S. Icelandic ovine Mycoplasma ovipneumoniae are variable bacteria that induce limited immune responses in vitro and in vivo. J Med Microbiol. 2018;67(10):1480-90.
  10. Rekha V, Rana R, Remesh A, Singh V. Mycoplasma arginini: High frequency involvement in goat pneumonia. Turk J Vet Anim Sci. 2017;41:393-9.
  11. Li Y, Jiang Z, Xue D, Deng G, Li M, Liu X, et al. Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway. BMC Microbiol. 2016;16(1):222.
  12. Azizi S, Tajbakhsh E, Rezaii AA, editors. The role of Mycoplasma ovipneumoniae and Mycoplasma arginini in pneumonic lungs of slaughtered sheep. 2011.
  13. Ibrahim N, El Beskawy MA, Elshafay D, Abd-Elmoaty A, Elnaker Y. Mycoplasma ovipneumonia: Isolation and molecular identification in diseased sheep flock in delta region. Assiut Vet Med J. 1999;64(158)
  14. Radostits O, Gay C, Hinchcliff K, Constable P. Veterinary medicine. A textbook of the disease of cattle, horses, sheep, pigs and goats. 10th ed. London: Baillier Tindal; 2007.
  1. Caswell JL, Williams KJ. Respiratory System. In: Maxie MG, editor. Jubb, Kennedy & Palmer's Pathology of Domestic Animals: Volume 2 (Sixth Edition): W.B. Saunders; 2016. p. 465-591.e4.
  2. Al-Dabhawe AH, Kadhim HM, Samaka HM. Molecular detection of infectious bronchitis virus and it is relation with avian influenza virus (H9) and Mycoplasma gallisepticum from different geographical regions in Iraq. Iraqi J Vet Sci. 2013;27(2):97-101.
  3. Jafar N, Noomi B. Detection of Mycoplasma gallisepticum and Mycoplasma synoviae by using of cultural and PCR technique. Iraqi J Vet Sci. 2019;33(2):469-73.
  4. Osman MM, Abu Elmakarem Mohamed M, N. Deif H, M. Osman K. Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt. J Appl Vet Sci. 2021;6(4):39-45.
  5. Abdel Halium M, Salib F, Marouf S, Abdel Massieh E. Isolation and molecular characterization of Mycoplasma spp. in sheep and goats in Egypt. Vet World. 2019;12(5):664-70.
  6. Smith B, Van Metre D, Pusterla N. Large Animal internal medicine. United States: Mosby Elsevier; 2019.
آمار
تعداد مشاهده مقاله: 66,548
تعداد دریافت فایل اصل مقاله: 58,670


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب