Acute and Subchronic Toxicity and Cytotoxicity of Saffron Corm Extract

Einafshar, Soodabeh; Tavakoli, Fatemeh; Hakimi, Zahra; Emami, Azadeh

doi:10.22092/jmpb.2022.358799.1480

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	Acute and Subchronic Toxicity and Cytotoxicity of Saffron Corm Extract
Journal of Medicinal plants and By-product
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 18 مهر 1401 اصل مقاله (1.14 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2022.358799.1480
نویسندگان
Soodabeh Einafshar¹؛ Fatemeh Tavakoli ²؛ Zahra Hakimi²؛ Azadeh Emami³
¹Agricultural Engineering Research Department, Khorasan Razavi Agricultural and Natural Resources and Education Center, AREEO, Mashhad, Iran
²Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
³Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
چکیده
Saffron corm is produced annually in large quantities in Iran. It contains some bioactive components like phenolic and antioxidative compounds. We extracted saffron corm by ultrasound-assisted solvent extraction method. The extraction yield and antioxidant properties, free radical scavenging ability, FRAP, and total phenolic compounds (TPC), were determined. To evaluate acute and subchronic toxicity, the extract was administered at three different doses of 1, 10, and 100 mg/kg to BALB/c male mice. Then, the rate of mortality and biochemical parameters such as LDH, ALT, AST, ALP, TG, cholesterol and creatinine were measured after 24 hours and 60 days respectively. Effect of extract on healthy (HFF) and cancerous cells (MCF-7 & HT-29) was evaluated using MTT assay. Results showed that the 40 min ultrasound-assisted extraction method with the highest extraction yield (0.98%), free radical scavenging inhibition (64.37%), and highest FRAP (643.51 µmolfeII.l^-1) and TPC (82.23 mg.ml^-1) could be selected as the most active saffron corm extract. According to our animal study after acute and subchronic administration of extract, no significant alteration of the levels of TG, cholesterol, ALP, ALT, AST and creatinine was observed, in mice. Cytotoxicity tests showed that saffron corm extract had no toxic effect on HFF cells, although it had toxic effects on MCF-7 & HT-29 cells. Conclusively, to our data, saffron corm extract had no significant acute and subchronic toxicity on BALB/c mice, and although it had toxic effects on cancer cells no cytotoxicity was seen on normal fibroblast cells.
کلیدواژه‌ها
Saffron corm؛ Ultrasound-assisted extraction؛ Acute Toxicity؛ Subchronic Toxicity؛ Cytotoxicity

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Acute and Subchronic Toxicity and Cytotoxicity of Saffron Corm Extract