اخبار و اعلانات

 آمار

تعداد نشریات283
تعداد نشریات سازمان81
تعداد شماره‌ها2,727
تعداد مقالات31,054
تعداد مشاهده مقاله28,192,054
تعداد دریافت فایل اصل مقاله16,254,947
Saeed, Ban, Yousief, M. Y, Abdulrda, A. J, Ayied, A. Y. (1402). Study of Local Black Iraqi Goats Genotypes for the Cytb Gene. سامانه مدیریت نشریات علمی, 78(3), 915-921. doi: 10.22092/ari.2022.359888.2499
Ban Saeed; M. Y Yousief; A. J Abdulrda; A. Y Ayied. "Study of Local Black Iraqi Goats Genotypes for the Cytb Gene". سامانه مدیریت نشریات علمی, 78, 3, 1402, 915-921. doi: 10.22092/ari.2022.359888.2499
Saeed, Ban, Yousief, M. Y, Abdulrda, A. J, Ayied, A. Y. (1402). 'Study of Local Black Iraqi Goats Genotypes for the Cytb Gene', سامانه مدیریت نشریات علمی, 78(3), pp. 915-921. doi: 10.22092/ari.2022.359888.2499
Saeed, Ban, Yousief, M. Y, Abdulrda, A. J, Ayied, A. Y. Study of Local Black Iraqi Goats Genotypes for the Cytb Gene. سامانه مدیریت نشریات علمی, 1402; 78(3): 915-921. doi: 10.22092/ari.2022.359888.2499

Study of Local Black Iraqi Goats Genotypes for the Cytb Gene

Archives of Razi Institute
مقاله 17، دوره 78، شماره 3، مرداد و شهریور 2023، صفحه 915-921    اصل مقاله (652.12 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359888.2499
نویسندگان
Ban Saeed* ؛ M. Y Yousief؛ A. J Abdulrda؛ A. Y Ayied
Animal Production Department, College of Agriculture, University of Basrah, Basrah, Iraq
چکیده
Goats are the earliest domesticated ruminants. The local goat, Capra hircus, is considered one of the most important animals globally to provide good livestock production under harsh environmental conditions. This study aimed to detect the genetic structures of the local Iraqi goats bred in the central and southern regions of the country and investigate the possibility of benefiting from their genetic structures to construct improvement programs for increasing the productivity of these animals. To this end, blood samples were taken from 15 domestic black goats. A total of 10 ml of each animal’s blood was placed in plastic containers of 10 ml. The DNA was extracted and sent to the laboratories of Juan Ju University, People’s Republic of China, to analyze the sequences of the nitrogenous bases of the Cytochrome b (Cytb) gene. The results showed the presence of a genetic morphology for a segment of 670 base pairs for all the studied samples, and 15 sequences of this strain were recorded in the gene bank under the following accession numbers (LC496353.1:1-LC496367.1:1). The sequences of the nitrogenous bases of this segment of the gene, which were registered in the gene bank of some international goat breeds, were used for comparison with the sequences of black Iraqi goats to analyze the phylogenetic tree, calculate the genetic distance, study haplotypes, and calculate neutrality. The results showed the presence of one mutation in the studied segment of the Cytb gene, with a size of 670 bp. The mutation in base 46 of the studied gene converted from the purine group to the pyrimidine group (the shift from the nitrogen leaders A<C) in all the studied samples. It led to the transformation of the amino acid Asparagine into Histidine, where 233 amino acids were obtained, dominated by the amino acid Isoleucine and Leucine, over other amino acids at a rate of 14.34% and 11.21%, respectively. The phylogenetic tree showed the existence of two main branches, one of which included the local black Iraqi goat breed, and the other included all the international breeds under comparison. It is concluded that the black Iraqi goat breed has a different origin from other breeds.
کلیدواژه‌ها
local black؛ Iraqi goats genotypes؛ Cytb gene
سایر فایل های مرتبط با مقاله
مراجع
  1. Mason IL, Mason IL. Evolution of domesticated animals. 1984.
  2. Pizarro M, Landi V, Navas González F, León J, Delgado J. Non-parametric analysis of the effects of αS1-casein genotype and parturition non-genetic factors on milk yield and composition in Murciano-Granadina goats. Ital J Anim Sci. 2019;18(1):1021-34.
  3. Al-Dabbagh ASS, OramarI RA, editors. A comparative study on milk yield and its composition between Black mountain and Damascus (Shami) goats raised at commercial farms. The 2nd Scientific Agricultural Conference; 2016.
  4. Gıda BM, Teşkilatı T. Food and Agriculture Organization of the United Nations (FAO),(2005).
  5. Mucha S, Mrode R, Coffey M, Kizilaslan M, Desire S, Conington J. Genome-wide association study of conformation and milk yield in mixed-breed dairy goats. J Dairy Sci. 2018;101(3):2213-25.
  6. Wajid A, Hussain T, Wasim M, Babar M, Anjum A, Shah S, et al. The future prospective of genomic biotechnology in animal breeding: their potential for livestock production in Pakistan. J Anim Plant Sci. 2013;23(4):944-55.
  7. Petrella G, Pati S, Gagliardi R, Rizzuti A, Mastrorilli P, La Gatta B, et al. Study of proteolysis in river buffalo mozzarella cheese using a proteomics approach. J Dairy Sci. 2015;98(11):7560-72.
  8. Kul BC, Ertugrul O. mtDNA diversity and phylogeography of some Turkish native goat breeds. 2011.
  9. Sepehri B, Seyedabadi HR, editors. Molecular analysis of Khalkhali goat population based on cytB region of mitochondrial DNA. Biological Forum; 2015: Satya Prakashan.
  10. Jiyanto J, Sutopo S, Kurnianto E. The genetic diversity of Kejobong goat based on Cytochrome B gene. J Indones Trop Anim Agric. 2014;39(2):75-82.
  11. Lestari DA, Purbowati E, Sutopo S, Kurnianto E. Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia. Vet World. 2018;11(8):1196.
  12. Pakpahan S, Artama WT, Widayanti R, Suparta I. Genetic characteristics and relationship in different goat populations of Indonesia based on Cytochrome B gene sequences. Asian J Anim Sci. 2016;10:29-38.
  13. Lestari D, Sutopo S, Kurnianto E. Amino acid diversity on the basis of cytochrome b gene in Kacang and Ettawa Grade goats. J Indones Trop Anim Agric. 2017;42(3):135-46.
  14. Chen S, Ma H, Chen G, Wang L. Complete mitochondrial genome of the Liuyang black goat and its phylogenetic relationship with other Caprinae. Genet Mol Res. 2016;15(2):1-11.
  15. Luikart G, Gielly L, Excoffier L, Vigne J-D, Bouvet J, Taberlet P. Multiple maternal origins and weak phylogeographic structure in domestic goats. Proc Natl Acad Sci. 2001;98(10):5927-32.
  16. Vakalounakis DJ, Fragkiadakis G. Genetic diversity of Fusarium oxysporum isolates from cucumber: differentiation by pathogenicity, vegetative compatibility, and RAPD fingerprinting. Phytopathology. 1999;89(2):161-8.
  1. Hall T, Biosciences I, Carlsbad C. BioEdit: an important software for molecular biology. GERF Bull Biosci. 2011;2(1):60-1.
  2. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547.
  3. Nei M, Takezaki N. Estimation of genetic distances and phylogenetic trees from DNA analysis. Proc 5th World Cong Genet Appl Livstock Prod. 1983;21(21):405-12.
  4. An T, Sun H, Li G, Zhao H, Wong PK. Differences in photoelectrocatalytic inactivation processes between E. coli and its isogenic single gene knockoff mutants: destruction of membrane framework or associated proteins? Appl Catal B: Environ. 2016;188:360-6.
  5. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30(12):2725-9.
  6. Kim J-H, Byun M-J, Ko Y-G, Kim S-W, Kim S-W, Do Y-J, et al. Phylogenetic analysis of Korean native goats based on the mitochondrial cytochrome b gene. J Anim Sci Technol. 2012;54(4):241-6.
  7. Zhang S, Gao X, Jiang Y, Shen Y, Xie H, Pan P, et al. Population validation of reproductive gene mutation loci and association with the litter size in Nubian goat. Arch Anim Breed. 2021;64(2):375-86.
  8. Nicoloso L, Bomba L, Colli L, Negrini R, Milanesi M, Mazza R, et al. Genetic diversity of Italian goat breeds assessed with a medium-density SNP chip. Genet Sel Evol. 2015;47(1):1-10.
  9. Sultana S, Mannen H, Tsuji S. Mitochondrial DNA diversity of Pakistani goats. Anim Genet. 2003;34(6):417-21.
  10. Petretto E, Dettori ML, Pazzola M, Manca F, Amills M, Vacca GM. Mitochondrial DNA diversity of the Sardinian local cattle stock. Sci Rep. 2022;12(1):1-7.
  11. Rodionov AN, Dotsev AV, Fomenko OY, Bakoev NF, Deniskova TE, Shakhin AV, et al. Complete mitochondrial genomes of Karchaev goat (Capra hircus). Mitochondrial DNA Part B. 2020;5(3):3627-8.
آمار
تعداد مشاهده مقاله: 1,269
تعداد دریافت فایل اصل مقاله: 250


counter
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب