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Taqi AL-Khazali, Maysaa, Mousa Hassan, Ban, Ahmed AbedIbrahim, Salam. (1401). Molecular Identification Of Candida albicans and C.dubliniensis Using Small Subunit rRNA Gene Sequence In Kerbala ,Iraq.. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2022.360086.2546
Maysaa Taqi AL-Khazali; Ban Mousa Hassan; Salam Ahmed AbedIbrahim. "Molecular Identification Of Candida albicans and C.dubliniensis Using Small Subunit rRNA Gene Sequence In Kerbala ,Iraq.". سامانه مدیریت نشریات علمی, , , 1401, -. doi: 10.22092/ari.2022.360086.2546
Taqi AL-Khazali, Maysaa, Mousa Hassan, Ban, Ahmed AbedIbrahim, Salam. (1401). 'Molecular Identification Of Candida albicans and C.dubliniensis Using Small Subunit rRNA Gene Sequence In Kerbala ,Iraq.', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2022.360086.2546
Taqi AL-Khazali, Maysaa, Mousa Hassan, Ban, Ahmed AbedIbrahim, Salam. Molecular Identification Of Candida albicans and C.dubliniensis Using Small Subunit rRNA Gene Sequence In Kerbala ,Iraq.. سامانه مدیریت نشریات علمی, 1401; (): -. doi: 10.22092/ari.2022.360086.2546

Molecular Identification Of Candida albicans and C.dubliniensis Using Small Subunit rRNA Gene Sequence In Kerbala ,Iraq.

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مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 05 آذر 1401  XML
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.360086.2546
نویسندگان
Maysaa Taqi AL-Khazali email 1؛ Ban Mousa Hassan2؛ Salam Ahmed AbedIbrahim3
1Department of Biology, College of Science, University of Kerbala, Iraq
2Department of Biology, College of Education, University of Kerbala, Iraq
3College of Pharmacy, University of Kerbala, Iraq
چکیده
This study was conducted to confirm the phenotypic diagnosis of the two species Candida albicans and C.dubliniensis that were previously isolated in a previous study from cases of oral candidiasis, using polymerase chain reaction and determination of the nitrogenous base sequences of the 18-srRNA products duplication using the primers NS1, NS8, The sequences of the multiple bases were analyzed using the Basic Local Blast Alignment Search Tool program (BLAST), which proved that the two diagnosed Candida strains belong to the two species C .albicans and C.dubliniensis respectively, and by comparing these sequences with the data available in the National Center for Biotechnology Information (NCBI) database we found the study isolate C.albicans showed a similar percentage of 99% with universal strains of C.albicans from Japan, Brazil, the United States, Germany, India, China, Pakistan and Egypt. While C.dubliniensis strain had the highest genetic similarity rate of 99% with C.dubliniensis isolate from the United States, Netherlands, France and Germany. The study strains were recorded in the gene bank database with the sequence code MZ574137 and MZ574410.1 for C .albicans and C.dubliniensis respectively. The results of the duplication of the 18 SrRNA region also showed the presence of variations between C.albicans and C.dubliniensis represented by the presence of three mutations of the first type and two mutations in the second type at different sites of the sequence.
کلیدواژه‌ها
Candida albicans؛ C.dubliniensis؛ BLAST؛ NCBI
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تعداد مشاهده مقاله: 17


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