The study included 60 samples of ewes blood were collected (50 ewes were lying down suffering from pregnancy Toxemia and 10 healthy ewes were considered as a control group), all of them were in the last month of pregnancy from the Awassi strain in different regions of Salah Aldin Governorate, for the period from the beginning of October /2021 until the end of February/2022, where the ages of the animals included in the study ranged from 2-8 years. Biochemical analysis determination of serum concentrations of all parameters by using the atomic absorption spectrophotometer except the BHBA and NEFA by ELISA method . The results of the clinical criteria tests for temperature, respiration and pulse showed a non significant differences (P<0.05) in the infected animals compared with the healthy group. Clinical signs which were represented by depression, loss of appetite, weight loss, lying down, odor of ketogenic bodies with breathing, and inability to walk, neurological signs, dental grinding, jaundice, blindness, bloat, dystocia, animal death and fetal death. The study showed from the results of the biochemical parameters tests in the blood a significant increase (P<0.05) in the parameters of the results of BHBA, NEFA, Triglycerides, Total bilirubin and liver enzymes, (ALT, AST, ALP, GGT) in the affected pregnancy toxemic animals compared with the control group, while a significant decrease (P<0.05) was observed in the parameters of Glucose, Cholesterol, Total protein, Albumin and Globulin in the affected animals compared to the healthy group. As for the study of the relationship of disease with oxidative stress criteria, the study noted a significant (P<0.05) increase in the level of malondialdehyde concentration and a significant (P<0.05) decrease in the level of the antioxidants glutathione and superoxide dismutase in the affected. |