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Salamat, N., Derakhshesh, N., Shiry, N., Alavinia, S.J.. (1401). Cytotoxic activities of Padina gymnospora and Acanthophora spicifera extracts against human breast cancer cell lines. سامانه مدیریت نشریات علمی, 21(6), 1527-1538. doi: 10.22092/ijfs.2023.128519
N. Salamat; N. Derakhshesh; N. Shiry; S.J. Alavinia. "Cytotoxic activities of Padina gymnospora and Acanthophora spicifera extracts against human breast cancer cell lines". سامانه مدیریت نشریات علمی, 21, 6, 1401, 1527-1538. doi: 10.22092/ijfs.2023.128519
Salamat, N., Derakhshesh, N., Shiry, N., Alavinia, S.J.. (1401). 'Cytotoxic activities of Padina gymnospora and Acanthophora spicifera extracts against human breast cancer cell lines', سامانه مدیریت نشریات علمی, 21(6), pp. 1527-1538. doi: 10.22092/ijfs.2023.128519
Salamat, N., Derakhshesh, N., Shiry, N., Alavinia, S.J.. Cytotoxic activities of Padina gymnospora and Acanthophora spicifera extracts against human breast cancer cell lines. سامانه مدیریت نشریات علمی, 1401; 21(6): 1527-1538. doi: 10.22092/ijfs.2023.128519

Cytotoxic activities of Padina gymnospora and Acanthophora spicifera extracts against human breast cancer cell lines

Iranian Journal of Fisheries Sciences
دوره 21، شماره 6، بهمن 2022، صفحه 1527-1538    اصل مقاله (954.64 K)
شناسه دیجیتال (DOI): 10.22092/ijfs.2023.128519
نویسندگان
N. Salamat* 1؛ N. Derakhshesh1؛ N. Shiry2؛ S.J. Alavinia3
1Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
2Iran Fisheries Organization, Administration of Khozestan Province, Abadan, Iran
3Department of Fisheries, Faculty of Natural Resource, University of Tehran, Karaj, Iran
چکیده
The resistance of cancerous cells to anti-cancer drugs is one of the most common problems in medicine and therefore, finding new anti-cancer compounds with the least side effects seems to be necessary. The present study was performed to investigate the anti-cancer potential of Padina gymnospora and Acanthophora spicifera, two native algae species of the Persian Gulf, in vitro. In this regard, methanol, chloroform, n-hexane, and ethyl acetate extracts of both algae species were added to cultivated MCF-7 cells at different concentrations (125, 250, 500, and 1000 μg/mL). 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay was used to determine the toxicity effects of algae extracts on MCF-7 cells. DNA isolation and agarose gel electrophoresis were also performed to assess DNA fragmentation induced by these two algae species. Based on the MTT results, the sensitivity of cultivated MCF-7 cells to P. gymnospora and A. spicifera extracts was increased in a dose-dependent manner. The highest concentration of methanolic extract of both algae species significantly affected the MCF-7 cells and led to the highest cell death. Moreover, the IC50 of P. gymnospora and A. spicifera methanolic extracts for the MCF-7 cells were equal to 557.78 and 910.61 μg/ml, respectively, which indicates P. gymnospora has more cytotoxic activity and anti-tumor potency. The lowest concentration of all types of algae extracts was not considerably cytotoxic to cultivated MCF-7 cells. The DNA fragmentation of MCF-7 cells was increased with increasing the concentration of the algal extracts. The highest amount of DNA fragmentation caused by 1000 μg/mL of P. gymnospora and A. spicifera extract; however, methanolic extract of P. gymnospora caused more DNA fragmentation in MCF-7 cells than A. spicifera.
کلیدواژه‌ها
Cytotoxic activity؛ Secondary metabolites Algae؛ MTT assay؛ MCF-7
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