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Evaluation of Molecular Epidemiology of IAPV in Several Regions of Iran | ||
| Archives of Razi Institute | ||
| مقاله 18، دوره 78، شماره 5، آذر و دی 2023، صفحه 1572-1578 اصل مقاله (439.97 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.32592/ARI.2023.78.5.1572 | ||
| نویسندگان | ||
| mojtaba moharrami* 1؛ Hossein Modirrousta2؛ Masoumeh Bagheri1 | ||
| 1Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, 31976-19751, Iran. | ||
| 2Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, 31976-19751, Iran.y | ||
| چکیده | ||
| In this study, the Israeli acute paralysis virus (IAPV), a single-stranded RNA virus, was investigated in honey bee colonies, which had a history of mortality, population decline, and parasitic diseases. Samples (adult honey bees) were collected from 328 apiaries from three provinces (Tehran, Alborz, and Mazandaran) of Iran to detect IAPV. After sample preparation, RNA was extracted and cDNA was synthesized to perform the reverse transcription polymerase chain reaction (RT-PCR) method using a PCR primer pair, and a 185 bp fragment was amplified. The results showed that out of 328 samples, 103 (31.4%) samples were positive, which were from Mazandaran (14.33%), Tehran (8.84%), and Alborz (8.23%) provinces. Subsequently, some of the positive samples were sequenced and a phylogenetic tree was drawn. The phylogenetic tree showed that the virus isolates were divided into two distinct groups, including one group that had a high similarity to the European acute bee paralysis virus (ABPV) and one group that had a high similarity to the Kashmir bee virus. In addition, the sequences of the samples in three regions were separated in a node from the strains of ABPV from Eastern Europe. Since the length of the branch between the Iranian sequences and the different strains of ABPV from Eastern Europe was short, it can be assumed that the sequences from Iran have a common ancestor with the mentioned strains of ABPV from Eastern Europe. | ||
| کلیدواژهها | ||
| Honey bee؛ IAPV؛ Phylogenetic tree؛ RT-PCR | ||
| عنوان مقاله [English] | ||
| اپیدمیولوژی مولکولی IAPV در زنبورستانهای سه استان ایران | ||
| چکیده [English] | ||
| در این مطالعه، ویروس فلجی اسرائیلی به عنوان یک ویروس RNA تک رشتهای، در کلنیهای زنبور عسل که سابقه مرگ و میر، کاهش جمعیت و بیماریهای انگلی داشتند، مورد بررسی قرار گرفت. در مجموع 328 نمونه زنبورستان بالغ از سه استان ایران (تهران، البرز و مازندران) برای تشخیص IAPV جمع آوری شد. پس از آمادهسازی نمونهها، RNA استخراج و cDNA برای انجام روش RT-PCR سنتز شد و قطعه 185 جفت باز تکثیر شد. نتایج نشان داد که از مجموع 328 نمونه، 103 نمونه (4/31%) مثبت ارزیابی شدند که سهم هریک از استانهای تهران معادل 84/8%، البرز معادل 23/8% و مازندران معادل 33/14% بود. سپس تعدادی از نمونه های مثبت توالی یابی شده و درخت فیلوژنتیک ترسیم شد. درخت فیلوژنتیک نشان داد که جدایههای ویروس به دو گروه مختلف تقسیم شدند، گروهی که شباهت زیادی به ویروس فلجی حاد زنبور عسل اروپایی داشتند و گروهی که شباهت زیادی با ویروس زنبور عسل کشمیر داشتند. همچنین، توالیهایی از نمونهها در سه منطقه در یک گره از سویههای ABPV از اروپای شرقی جدا شدند. با توجه به اینکه طول شاخه بین توالیهای ایرانی و سویههای مختلف ABPV از اروپای شرقی کوتاه بود، میتوان نتیجه گرفت که توالیهای ایران جد مشترکی با سویههای مذکور در ABPV از اروپای شرقی دارند. | ||
| کلیدواژهها [English] | ||
| زنبور عسل, IAPV RT-PCR درخت فیلوژنی | ||
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| مراجع | ||
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