| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شماره‌ها | 3,085 |
| تعداد مقالات | 34,432 |
| تعداد مشاهده مقاله | 48,786,485 |
| تعداد دریافت فایل اصل مقاله | 79,282,825 |
شناسایی کنههای ناقل Cercopithifilaria و Hepatozoon در سگهای مناطق مختلف ایران | ||
| نامه انجمن حشره شناسی ایران | ||
| دوره 43، شماره 1، اردیبهشت 1402، صفحه 53-61 اصل مقاله (1011.12 K) | ||
| نوع مقاله: مقاله کامل، فارسی | ||
| شناسه دیجیتال (DOI): 10.52547/JESI.43.1.6 | ||
| نویسندگان | ||
| زهرا بحیرایی1؛ علیرضا سازمند* 1؛ علیرضا نوریان1؛ دومنیکو اوترانتو2 | ||
| 1گروه آموزشی پاتوبیولوژی، دانشکده پیرادامپزشکی، دانشگاه بوعلی سینا، همدان، ایران | ||
| 2انستیتو انگل شناسی، دپارتمان دامپزشکی، دانشگاه باری آلدو مورو، ایتالیا | ||
| چکیده | ||
| پیرو یافتن تک‌یاختهی Hepatozoon canis و نماتود Cercopithifilaria bainae در سگهای ایران، این مطالعه با هدف تشخیص ناقلین بالقوهی این دو انگل در میان کنههای جمعآوری شده از سگهای ۵ استان ایران انجام شد. از مهر ماه 1397 تا خرداد ماه 1401 تعداد 222 قلاده سگ صاحبدار و 541 قلاده سگ ولگرد از استانهای همدان (305 قلاده)، کرمانشاه (227 قلاده)، یزد (80 قلاده)، خوزستان (71 قلاده) و مازندران (80 قلاده) جهت آلودگی به کنه مورد بررسی قرار گرفتند. کنههای جمع آوری شده (3339 عدد) از نظر ریختشناسی شناسایی شده و کنههای بالغ با استفاده از روشهای PCR معمولی (119 کنه)، کالبدگشایی (106 کنه) و بررسی میکروسکوپی اسلاید تهیه شده از مقاطع بافتی فرمالینه (102 کنه) آزمایش شدند. در مجموع 169 قلاده سگ (1/22%) به کنههای سخت آلودگی داشتند. فراوانی آلودگی در همدان 4/32%، کرمانشاه 3/27% و یزد 10% بود درحالیکه آلودگی در سگهای مورد بررسی در مازندران و خوزستان مشاهده نشد. اغلب کنههای نر جمعآوری شده متعلق به گونهRhipicephalus sanguineus (3/89%) و Rhipicephalus turanicus بودند اما یک قلاده سگ از همدان به ۲ کنهی Hyalomma asiaticum (۱ نر و ۱ ماده) و ۱ کنهی نر Hyalomma excavatumآلوده بود. طبق بررسی مولکولی فراوانی Cercopithifilaria،Hepatozoon و آلودگی همزمان به هر دو انگل در کنههای Rhipicephalusبه ترتیب 28/14%، 7/6% و 68/1% بود. انگلهای مورد مطالعه در کالبدگشایی و بررسی هیستولوژیک کنهها مشاهده نشدند. نتایج توالییابی و آنالیز فیلوژنتیکی مؤید حضور C. bainae و H. canis در کنههای گونهRh. sanguineus و Rh. turanicus بود. نتایج این مطالعه مؤید آن است که هر دو گونهی کنه در اپیدمیولوژی Cercopithifilaria و Hepatozoon در ایران نقش دارند. | ||
| کلیدواژه‌ها | ||
| پاتوژنهای منتقله از ناقل؛ ریپی سفالوس تورانیکوس؛ ریپی سفالوس سنگویینئوس؛ سرکوپیتی فیلاریا؛ هپاتوزوئون | ||
| عنوان مقاله [English] | ||
| Identification of Hepatozoon and Cercopithifilaria in ticks infesting dogs of different regions of Iran | ||
| نویسندگان [English] | ||
| Zahra Bahiraei1؛ Alireza Sazmand1؛ Alireza Nourian1؛ Domenico Otranto2 | ||
| 1Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran | ||
| 2Department of Veterinary Medicine, University of Bari, Bari, Italy | ||
| چکیده [English] | ||
| Following previous detection of Hepatozoon canis and Cercopithifilaria bainae in dogs from Iran, this study aimed to identify potential tick vectors collected from dogs from five provinces of Iran. From October 2018 to September 2021, a total of 222 owned and 541 sheltered dogs from Hamedan (n=305), Kermanshah (n = 227), Yazd (n =80), Khouzestan (n =71), and Mazandaran (n =80) were examined for ticks. Collected ticks (n =3339) were identified morphologically, and adults were screened for the detection of Hepatozoon and Cercopithifilaria either with conventional PCR (n=119 ticks), tick dissection (n=106), or microscopical examination after fixation in formalin (n =102). A total of 169 dogs (22.1%) were infested with ticks. Tick infestation rate varied in different provinces (i.e., 32.4% in Hamedan, 27.3% in Kermanshah, 10.0% in Yazd), but no ticks were collected in dogs from Mazandaran and Khouzestan. The majority of male ticks were identified as Rhipicephalus sanguineus sensu lato (89.3%) and Rhipicephalus turanicus, but one dog from Kermanshah was infested with Hyalomma asiaticum (1 male and 1 female) and Hyalomma excavatum (1 male). At the molecular examination, Cercopithifilaria was detected in 14.28%, Hepatozoon in 6.7% ticks, and both parasites in 1.68% of Rhipicephalus ticks. Parasites were not observed at tick dissection and histological examinations. Nucleotide sequencing and phylogenetic analyses revealed C. bainae and H. canis in R. sanguineus sensu lato and R. turanicus ticks. Data suggests that both tick species above may play a role in the epidemiology of Cercopithifilaria and Hepatozoon in Iran. | ||
| کلیدواژه‌ها [English] | ||
| Tick, Filarial nematode, Protozal parasite, Iran, Rhipicephalus, vector-borne pathogens | ||
| مراجع | ||
|
Amoli, A., Rahmani, A., Khoshnegah, J. & Razmi, Gh. R. (2012) A preliminary parasitological survey of Hepatozoon spp. infection in dogs in Mashhad, Iran. Iranian Journal of Parasitology 7(4), 99-103. Angelou, A., Latrofa, M. S., Annoscia, G., Symeonidou, I., Theodoridis, A., Polizopoulou, Z. S., Otranto, D. & Papadopoulos, E. (2020) Cercopithifilaria species in dogs and ticks from Greece. Parasitology Research 119(10), 3391-3400. doi: https://doi.org/10.1007/s00436-020-06784-3 Bahrami, A. M., Doosti, A. & Ahmady Asbchin, S. (2012) Cat and dogs ectoparasite infestations in Iran and Iraq boarder line area. World Applied Sciences Journal 18(7), 884-889.https://doi.org/10.5829/idosi.wasj.2012.18.07.1376 Baneth, G., Samish, M., Aleksefv, E., Aroch, I. & Shkap, V. (2001) Transmission of Hepatozoon canis to dogs by naturally-fed or percutaneously-injected Rhipicephalus sanguineus ticks. Journal of Parasitology 87(3), 606-611. https://doi.org/10.1645/0022-3395(2001)087[0606:TOHCTD]2.0.CO;2 Baneth, G., Samish, M. & Shkap, V. (2007) Life cycle of Hepatozoon canis (Apicomplexa: Adeleorina: Hepatozoidae) in the tick Rhipicephalus sanguineus and domestic dog (Canis familiaris). Journal of Parasitology 93(2), 283-299. https://doi.org/10.1645/GE-494R.1 Bezerra-Santos, M. A., de Macedo, L. O., Nguyen, V. L., Manoj, R. R. S., Laidoudi, Y., Latrofa, M. S., Beugnet, F. & Otranto, D. (2022) Cercopithifilaria spp. in ticks of companion animals from Asia: new putative hosts and vectors. Ticks and Tick-borne Diseases 13(4), 101957. https://doi.org//doi.org/10.1016/j.ttbdis.2022.101957 Brianti, E., Otranto, D., Dantas-Torres, F., Weigl, S., Latrofa, M. S., Dantas-Torres, F., Giannelli, A., Gaglio, G., Napoli, E., Brucato, G., Cauquil, L. & Giannetto, S. (2012) Rhipicephalus sanguineus (Ixodida, Ixodidae) as intermediate host of a canine neglected filarial species with dermal microfilariae. Veterinary Parasitology 183(3-4), 330-337. https://doi.org/10.1016/j.vetpar.2011.07.031 Casiraghi, M., Anderson, T. J. C., Bandi, C., Bazzocchi, C. & Genchi, C. (2001) A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts. Parasitology 122(1), 93-103. https://doi.org/10.1017/s0031182000007149 Dantas-Torres, F. (2008) The brown dog tick, Rhipicephalus sanguineus (Latreille, 1806)(Acari: Ixodidae): from taxonomy to control. Veterinary Parasitology 152(3-4), 173-185. https://doi.org/10.1016/j.pt.2022.08.011 Dantas-Torres, F. (2010) Biology and ecology of the brown dog tick, Rhipicephalus sanguineus. Parasites and Vectors 3(1), 26. https://doi.org/doi.org/10.1186/1756-3305-3-26 Dantas-Torres, F., Latrofa, M. S., Weigl, S., Tarallo, V. D., Lia, R. P. & Otranto, D. (2011) Hepatozoon canis infection in ticks during spring and summer in Italy. Journal of Parasitology 93(2), 283-299. https://doi.org/10.1007/s00436-011-2544-8 Dantas-Torres, F. & Otranto, D. (2022) Rhipicephalus sanguineus (Brown dog tick). Trends in Parasitology 38(11), 993-994. https://doi.org//doi.org/10.1016/j.pt.2022.08.011 De Miranda, R. L., de Castro, J. R., Olegário, M. M. M., Beletti, M. E., Mundim, A. V., O’Dwyer, L. H., Eyal, O., Talmi-Frank, D., Cury, M. C. & Baneth, G. (2011) Oocysts of Hepatozoon canis in Rhipicephalus (Boophilus) microplus collected from a naturally infected dog. Veterinary Parasitology 177(3-4), 392-396. https://doi.org/10.1016/j.vetpar.2011.01.044 Ebrahimzade, E., Fattahi, R. & Ahoo, M. B. (2016) Ectoparasites of stray dogs in Mazandaran, Gilan and Qazvin Provinces, North and center of Iran. Journal of Arthropod-Borne Diseases 10(3), 366-371. Estrada-Peña, A., Mihalca, A. D. & Petney, T. N. (2017) Ticks of Europe and North Africa: A Guide to Species Identification (A. Estrada-Peña, Mihalca, A. D., Petney, T. N., Ed. 1st ed. ed.). Springer. 10.1007/978-3-319-63760-0 Gabrielli, S., Giannelli, A., Brianti, E., Dantas-Torres, F., Bufalini, M., Fraulo, M., La-Torre Ramos, F. R. A. N., Cantacessi, C., Latrofa, M. S., Cancrini, G. & Otranto, D. (2014) Chronic polyarthritis associated to Cercopithifilaria bainae infection in a dog. Veterinary Parasitology 205(1-2), 401-404. https://doi.org/10.1016/j.vetpar.2014.06.027 Giannelli, A., Lia, R. P., Annoscia, G., Buonavoglia, C., Lorusso, E., Dantas-Torres, F., Baneth, G. & Otranto, D. (2017) Rhipicephalus turanicus, a new vector of Hepatozoon canis. Parasitology 144(6), 730-737. Hodžić, A., Alić, A., Fuehrer, H. P., Harl, J., Wille-Piazzai, W. & Duscher, G. G. (2015) A molecular survey of vector-borne pathogens in red foxes (Vulpes vulpes) from Bosnia and Herzegovina. Parasites & Vectors 8(1), 88-88. https://doi.org/10.1186/s13071-015-0692-x Hosseini-Chegeni, A., Tavakoli, M. & Telmadarraiy, Z. (2019) The updated list of ticks (Acari: Ixodidae & Argasidae) occurring in Iran with a key to the identification of species. Systematic and Applied Acarology 24(11), 2133-2166. https://doi.org/10.11158/saa.24.11.8 Iatta, R., Sazmand, A., Nguyen, V. L., Nemati, F., Bahiraei, Z., Mazhar Ayaz, M., Giannico, A., Dantas-Torres, F. & Otranto, D. (2021) Vector-borne pathogens in dogs of different regions of Iran and Pakistan. Parasitology Research 120(12), 4219-4228. https://doi.org/10.1007/s00436-020-06992-x Ionică, A. M., D’Amico, G., Mitková, B., Kalmár, Z., Annoscia, G., Otranto, D., Modri, D. & Mihalca, A. D. (2014) First report of Cercopithifilaria spp. in dogs from Eastern Europe with an overview of their geographic distribution in Europe. Parasitology Research 113(7), 2761-2764. https://doi.org//doi.org/10.1007/s00436-014-3931-8 Jafari-Shoorijeh, S., Rowshan-Ghasrodashti, A., Tamadon, A., Moghaddar, N. & Behzadi, M. A. (2008) Seasonal frequency of ectoparasite infestation in dogs from Shiraz, Southern Iran. Turkish Journal of Veterinary and Animal Sciences 32(4), 309-313. Jamshidi, S., Maazi, N., Ranjbar-Bahadori, S., Rezaei, M., Morakabsaz, P. & Hosseininejad, M. (2012) A survey of ectoparasite infestation in dogs in Tehran, Iran. Revista Brasileira de Parasitologia Veterinária 21(3), 326. https://doi.org/10.1590/S1984-29612012000300030 Khazeni, A., Telmadarraiy, Z., Oshaghi, M. A., Mohebali, M., Zarei, Z. & Abtahi, S. M. (2013) Molecular detection of Ehrlichia canis in ticks population collected on dogs in Meshkin-Shahr, Ardebil Province, Iran. Journal of Biomedical Science and Engineering 6, 1-5. http://dx.doi.org/10.4236/jbise.2013.67A3001 Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33(7), 1870-1874. https://doi.org/10.1093/molbev/msw054 Lade, B., Patil, A. & Paikrao, H. (2014) Efficient genomic DNA extraction protocol from medicinal rich Passiflora foetida containing high level of polysaccharide and polyphenol. SpringerPlus 3(1), 1-7. https://doi.org/10.1186/2193-1801-3-457 Latrofa, M. S., Dantas-Torres, F., Giannelli, A. & Otranto, D. (2014) Molecular detection of tick-borne pathogens in Rhipicephalus sanguineus group ticks. Ticks and Tick-borne Diseases 5(6), 943-946. https://doi.org/10.1016/j.actatropica.2022.106582 Latrofa, M. S., Weigl, S., Dantas-Torres, F., Annoscia, G., Traversa, D., Brianti, E. & Otranto, D. (2012) A multiplex PCR for the simultaneous detection of species of filarioids infesting dogs. Acta Tropica 122(1), 150-154. https://doi.org/10.1016/j.actatropica.2012.01.006 Maia, C., Casero, M., Annoscia, G., Latrofa, M. S., Colella, V., Pereira, A., Azevedo, F. & Otranto, D. (2017) Cercopithifilaria sp. II in Vulpes vulpes: new host affiliation for an enigmatic canine filarioid. Parasitology Research 116(1), 441-443. https://doi.org/10.1007/s00436-016-5308-7 Manoj, R. R. S., Iatta, R., Latrofa, M. S., Capozzi, L., Raman, M., Colella, V. & Otranto, D. (2020) Canine vector-borne pathogens from dogs and ticks from Tamil Nadu, India. Acta Tropica 203, 105308. https://doi.org/10.1016/j.actatropica.2019.105308 Minabaji, A., Moshaverinia, A. & Khoshnegah, J. (2020) Frequency of ectoparasite infestation in dogs in Mashhad, Northeast Iran. Journal of Veterinary Research 75(3), 280-287. https://doi.org/10.22059/jvr.2019.274542.2894 Mirani, F., Yakhchali, M. & Naem, S. (2017) A study on ectoparasites fauna of dogs in suburbs of Ghilanegharb, Kermanshah province, Iran. Journal of Faculty of Veterinary Medicine University of Tehran 72(1), 7-14 [Article in Persian with English abstract]. https://doi.org/10.22059/jvr.2017.61285 Mosallanejad, B., Alborzi, A. R. & Katvandi, N. (2012) A Survey on ectoparasite infestations in companion dogs of Ahvaz District, South-west of Iran. Jornal of Arthropod-Borne Diseases 6(1), 70-78. Murata, T., Inoue, M., Taura, Y., Nakama, S., Abe, H. & Fujisaki, K. (1995) Detection of Hepatozoon canis oocyst from ticks collected from the infected dogs. Journal of Veterinary Medical Science 57(1), 111-112. https://doi.org/10.22092/ari.2017.110293.1125 Nava, S., Beati, L., Venzal, J. M., Labruna, M. B., Szabó, M. P., Petney, T., Saracho-Bottero, M. N., Tarragona, E. L., Dantas-Torres, F. & Silva, M. M. S. (2018) Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization. Ticks and Tick-borne Diseases 9(6), 1573-1585. https://doi.org//doi.org/ 10.1016/j.ttbdis.2018.08.001 O’Dwyer, L. H. (2011) Brazilian canine hepatozoonosis. Revista Brasileira de Parasitologia Veterinária 20(3) 181-193. https://doi.org/10.1590/s1984-29612011000300002 Otranto, D., Brianti, E., Abramo, F., Gaglio, G., Napoli, E., Latrofa, M. S., Ramos, R. A., Dantas-Torres, F. & Bain, O. (2012) Cutaneous distribution and localization of Cercopithifilaria sp. microfilariae in dogs. Veterinary Parasitology 190(1-2), 143-150. https://doi.org/10.1016/j.vetpar.2012.05.016 Otranto, D., Brianti, E., Dantas-Torres, F., Miro, G., Latrofa, M. S., Mutafchiev, Y. & Bain, O. (2013) Species diversity of dermal microfilariae of the genus Cercopithifilaria infesting dogs in the Mediterranean region. Parasitology 140(1) 99-108. https://doi.org/10.1017/S0031182012001357 Otranto, D., Brianti, E., Latrofa, M. S., Dantas-Torres, F., Giannelli, A., Annoscia, G., Weigl, S., Lia, R. P., Gaglio, G., Napoli, E., Giannetto, S. & Papadopoulos, E. (2012) On a Cercopithifilaria sp. transmitted by Rhipicephalus sanguineus: a neglected, but widespread filarioid of dogs. Parasites & Vectors 5(1), 1. https://doi.org/10.1186/1756-3305-5-1 Otranto, D., Huchet, J. B., Giannelli, A., Callou, C. & Dantas-Torres, F. (2014) The enigma of the dog mummy from Ancient Egypt and the origin of ‘Rhipicephalus sanguineus’. Parasites & Vectors 7, 2. https://doi.org/10.1186/1756-3305-7-2 Otranto, D., Varcasia, A., Solinas, C., Scala, A., Brianti, E., Dantas-Torres, F., Annoscia, G., Martin, C., Mutafchiev, Y. & Bain, O. (2013) Redescription of Cercopithifilaria bainae Almeida & Vicente, 1984 (Spirurida, Onchocercidae) from a dog in Sardinia, Italy. Parasites and Vectors 6(1), 132. https://doi.org/doi.org/10.1186/1756-3305-6-132 Petit, G., Bain, O., Cassone, J. & Seureau, C. (1988) La filaire Cercopithifilaria roussilhonichez la tique vectrice. Annales de Parasitologie Humaine et Comparée 63(4), 296-302. https://doi.org/doi:10.1051/parasite/1988634296 Ramos, R. A. N., Giannelli, A., Lia, R. P., Brianti, E., Tarallo, V. D., Breitshwerdt, E. B., Dantas-Torres, F., Stanneck, D. & Otranto, D. (2014) Incidence of Cercopithifilaria bainae in dogs and probability of co-infection with other tick-borne pathogens. PLoS One 9(2), e88198. http://dx.doi.org/10.1371/journal.pone.0088198 Ramos, R. A. N., Giannelli, A., Brianti, E., Annoscia, G., Cantacessi, C., Dantas-Torres, F. & Otranto, D. (2013) Tick vectors of Cercopithifilaria bainae in dogs: Rhipicephalus sanguineus sensu lato versus Ixodes ricinus. Parasitology Research 112(8), 3013-3017. https://doi.org/10.1007/s00436-013-3474-4 Santos, M. A. B., de Macedo, L. O., Otranto, D., do Nascimento Ramos, C. A., do Rêgo, A. G. d. O., Giannelli, A., Alves, L. C., de Carvalho, G. A. & Ramos, R. A. N. (2018) Screening of Cercopithifilaria bainae and Hepatozoon canis in ticks collected from dogs of Northeastern Brazil. Acta Parasitologica 63(3), 605-608. https://doi.org/10.1515/ap-2018-0069 Sazmand, A., Bahiraei, Z., Nemati, F., Annoscia, G., Bezerra-Santos, M. A., Nayebzadeh, H., Salemi, A. M., Mousavi, S. M., Sajjadi, S. M. & Otranto, D. (2022) Dermal microfilariae of dogs, jackals and cats in different regions of Iran. Parasites & Vectors 15, 28. https://doi.org/10.1186/s13071-021-05141-2 Shamshiri, Z., Goudarztalejerdi, A., Zolhavarieh, S. M., Kamalpour, M. & Sazmand, A. (2022) Molecular Identification of Bartonella species in dogs and arthropod vectors in Hamedan and Kermanshah, Iran. Iranian Veterinary Journal, In-Press. https://doi.org/10.22055/ivj.2022.325115.2436 Šlapeta, J., Chandra, S. & Halliday, B. (2021) The “tropical lineage” of the brown dog tick Rhipicephalus sanguineus sensu lato identified as Rhipicephalus linnaei. International Journal for Parasitology 51(6), 431-436. https://doi.org/10.1016/j.ijpara.2021.02.001 Šlapeta, J., Halliday, B., Chandra, S., Alanazi, A. D. & Abdel-Shafy, S. (2022) Rhipicephalus linnaei (Audouin, 1826) recognised as the “tropical lineage” of the brown dog tick Rhipicephalus sanguineus sensu lato: neotype designation, redescription, and establishment of morphological and molecular reference. Ticks and Tick-borne Diseases 102024. http://dx.doi.org/10.1016/j.ttbdis.2018.08.001 Šlapeta, J., Halliday, B., Dunlop, J. A., Nachum-Biala, Y., Salant, H., Ghodrati, S., Modrý, D. & Harrus, S. (2023) The “southeastern Europe” lineage of the brown dog tick Rhipicephalus sanguineus (sensu lato) identified as Rhipicephalus rutilus Koch, 1844: Comparison with holotype and generation of mitogenome reference from Israel. Current Research in Parasitology & Vector-borne Diseases, 3, 100118. https://doi.org/10.1016/j.crpvbd.2023.100118 Soltani, R. & Dalimi, A. (2018) A molecular study on Hepatozoon canis infection in dogs in Tehran (Iran). Archives of Razi Institute 73(4), 257-263. https://doi.org/10.22092/ari.2017.110293.1125 Uni, S., Bain, O., Fujita, H., Matsubayashi, M., Fukuda, M. & Takaoka, H. (2013) Infective larvae of Cercopithifilaria spp. (Nematoda: Onchocercidae) from hard ticks (Ixodidae) recovered from the Japanese serow (Bovidae). Parasite 20(1), 1-7. http://dx.doi.org/10.1051/parasite/2012001 Walker, J. B., Keirans, J. E. & Horak, I. G. (2000) The Genus Rhipicephalus (Acari, Ixodidae): A Guide to the Brown Ticks of the World Revised, Cambridge University Press. https://doi.org/doi.org/10.1017/CBO9780511661754 Zintl, A., Finnerty, E. J., Murphy, T. M., de Waal, T. & Gray, J. S. (2011) Babesias of red deer (Cervus elaphus) in Ireland. Veterinary Research 42(1), 7. https://doi.org/10.1186/1297-9716-42-7 | ||
|
آمار تعداد مشاهده مقاله: 836 تعداد دریافت فایل اصل مقاله: 724 |
||