1Department of Agriculture, Payam Noor University, Tehran, Iran
2Department of Biology, Faculty of Science, Arak University, P.O.Box: 879, Arak, Iran
3Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
4Gene Bank Department, Research Institute of Forests and Rangelands, Tehran, Iran
Iran has different environmental conditions due to specific geographical. Therefore, exploring essential oil and flavonoids components among different medicinal may be caused discoveries about valuable components. The present study aimed to study flavonoids profile and essential oils composition of four Lamiaceae species i.e. Stachys lavandulifolia, Teucrium polium, Thymus daenensis, and Ziziphora clinopodioides from Oshtorankoh located on Zagros Mountains, Iran. The plants were collected during 2014-2015 and were identified using available references. A chemical study using two-dimensional paper chromatography (2-DPC) and thin-layer chromatography (TLC) showed that the four studied species contained flavone C and C-/O glucosides and flavonoid sulfates. The result of the present study revealed that T. polium had high levels of many flavonoids such as apigenin, genistein, isorhamnetin, kaempferol, quercetin, rutin, andvitexin compared to the other studied plants. According to the results of GC/MS analysis, S. lavandulifolia was rich in α‑pinene (21.16%) and 1,8‑cineol (16.95%), T. polium in α‑pinene (12.21%) and sabinene (9.55%), T. daenensis in γ‑Terpinene (36.98%) and p‑Cymene (26.87%), and Z.clinopodioides in piperitone (16.84%) and 1,8‑cineol (16.11%) components.