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Mirzaei, Habibollah, Azaran, Azarakhsh, Mahmoudvand, Shahab, Shokri, Somayeh, Bagheri, Shahram, Ramezani, Ali, Jalilian, Shahram. (1402). Prevalence of Epstein-barr Virus (EBV) among Patients with Oral Squamous Cell Carcinoma from Ahvaz, Iran: A Case-Control Study. سامانه مدیریت نشریات علمی, 78(5), 1495-1502. doi: 10.32592/ARI.2023.78.5.1495
Habibollah Mirzaei; Azarakhsh Azaran; Shahab Mahmoudvand; Somayeh Shokri; Shahram Bagheri; Ali Ramezani; Shahram Jalilian. "Prevalence of Epstein-barr Virus (EBV) among Patients with Oral Squamous Cell Carcinoma from Ahvaz, Iran: A Case-Control Study". سامانه مدیریت نشریات علمی, 78, 5, 1402, 1495-1502. doi: 10.32592/ARI.2023.78.5.1495
Mirzaei, Habibollah, Azaran, Azarakhsh, Mahmoudvand, Shahab, Shokri, Somayeh, Bagheri, Shahram, Ramezani, Ali, Jalilian, Shahram. (1402). 'Prevalence of Epstein-barr Virus (EBV) among Patients with Oral Squamous Cell Carcinoma from Ahvaz, Iran: A Case-Control Study', سامانه مدیریت نشریات علمی, 78(5), pp. 1495-1502. doi: 10.32592/ARI.2023.78.5.1495
Mirzaei, Habibollah, Azaran, Azarakhsh, Mahmoudvand, Shahab, Shokri, Somayeh, Bagheri, Shahram, Ramezani, Ali, Jalilian, Shahram. Prevalence of Epstein-barr Virus (EBV) among Patients with Oral Squamous Cell Carcinoma from Ahvaz, Iran: A Case-Control Study. سامانه مدیریت نشریات علمی, 1402; 78(5): 1495-1502. doi: 10.32592/ARI.2023.78.5.1495

Prevalence of Epstein-barr Virus (EBV) among Patients with Oral Squamous Cell Carcinoma from Ahvaz, Iran: A Case-Control Study

Archives of Razi Institute
مقاله 12، دوره 78، شماره 5، آذر و دی 2023، صفحه 1495-1502    اصل مقاله (280.47 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2023.78.5.1495
نویسندگان
Habibollah Mirzaei1؛ Azarakhsh Azaran1؛ Shahab Mahmoudvand1؛ Somayeh Shokri1؛ Shahram Bagheri2؛ Ali Ramezani1؛ Shahram Jalilian* 1
1Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2Department of Pathology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Epstein-Barr virus (EBV), one of the most significant causes of lymphoid and epithelial cancers, has been linked to oral carcinogenesis; however, this etiological association remains controversial. To investigate this association, the present study aimed to determine the prevalence of EBV in cancerous and non-cancerous oral tissues from Ahvaz, Iran. In total, 164 blocks of formalin-fixed paraffin-embedded tissues from oral squamous cell carcinoma (OSCC), including 76 tongue squamous cell carcinomas and 88 non-cancerous tongue tissues, were collected from Ahvaz Imam Khomeini Hospital, Ahvaz, Iran, from December 2014 to March 2019, for this case-control study. The tissues were cut into 15-μm-thick sections, and DNA was extracted using a solution of Phenol, Chloroform, and Isoamyl Alcohol. The EBV detection and typing were performed using nested polymerase chain reaction. The EBV was detected in 9 (5.48%) out of the 164 samples studied, including 4 (5.26%) of the 76 SCC cases and 5 (5.68%) of the 88 samples in the control group (P>0.05). The EBV was positive in 2.40% of the 83 male and 8.6% of the 81 female samples (P>0.05). In terms of the histological grades of the case group, 3 (3/57) and 1 (1/13) of the EBV-positive samples were well and moderately differentiated, respectively (P>0.05). For EBV typing, the 9 EBV-positive samples were tested, and it was found that 2 and 7 of the cases were EBV type I and II, respectively. Results of the current study demonstrated the low frequency of EBV in Iranian patients with OSCC, with EBV type II predominating. Further studies are required to clarify the association between EBV and OSCC.
کلیدواژه‌ها
Epstein-barr virus؛ Oral squamous cell carcinoma؛ Nested Polymerase Chain Reaction؛ Prevalence
عنوان مقاله [English]
شیوع ویروس اپشتین بار (EBV) در بیماران مبتلا به کارسینوم سلول سنگفرشی دهان در اهواز، ایران : مطالعه مورد شاهدی
چکیده [English]
ویروس اپشتین بار (EBV)، یکی از مهم‌ترین علل سرطان‌های لنفوئیدی و اپیتلیال، با سرطان‌زایی دهان مرتبط است، اما این ارتباط علت‌شناختی همچنان بحث‌برانگیز است. برای بررسی این ارتباط، مطالعه حاضر با هدف تعیین شیوع EBV در بافت‌های سرطانی و غیرسرطانی دهان شهر اهواز انجام شد. در مجموع 164 بلوک از بافت پارافینی فیکس شده با فرمالین (FFPE) از کارسینوم سلول سنگفرشی دهان (OSCC) شامل 76 کارسینوم سلول سنگفرشی زبان و 88 بافت غیر سرطانی زبان از بیمارستان امام خمینی اهواز جمع‌آوری شد. ، از دسامبر 2014 تا مارس 2019، برای این مطالعه مورد-شاهدی. بافت ها به بخش هایی به ضخامت 15 میکرومتر برش داده شدند و DNA با استفاده از محلول فنل، کلروفرم و ایزوآمیل الکل استخراج شد. تشخیص و تایپ EBV با استفاده از nested-PCR انجام شد. EBV در 9 مورد (5.48%) از 164 نمونه مورد مطالعه، شامل 4 (5.26%) از 76 مورد SCC و 5 (5.68%) از 88 گروه کنترل شناسایی شد (05/0P>). EBV در 2.40 درصد از 83 نمونه مرد و 8.6 درصد از 81 نمونه زن مثبت بود (P>0.05). از نظر درجات بافت شناسی گروه مورد، 3 (3/57) و 1 (1/13) از نمونه های EBV مثبت به ترتیب خوب و متوسط تمایز یافتند (05/0 P>). برای تایپ ، 9 نمونه EBV مثبت مورد آزمایش قرار گرفتند که 2 و 7 مورد به ترتیب EBV نوع I و II بودند. مطالعه حاضر فراوانی پایین EBV را در بیماران ایرانی مبتلا به OSCC نشان داد که EBV نوع II غالب بود. مطالعات بیشتری برای روشن شدن ارتباط بین EBV و OSCC مورد نیاز است.
کلیدواژه‌ها [English]
ویروس اپشتین بار (ایی. بی . وی), کارسینوم سلول سنگفرشی دهان (او.اس.سی .سی), نستذ پی سی آر, شیوع
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مراجع
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