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Salman Hamza, A, Traef Ali, Q, Hadi Farman, R. (1402). The Healing Effect of Biodegradable Scaffolds Treated with Bone-Marrow Obtained Mesenchymal Stem Cells on Major Tendon Damage in the Dog as a Model. سامانه مدیریت نشریات علمی, 78(3), 889-898. doi: 10.22092/ari.2022.359751.2470
A Salman Hamza; Q Traef Ali; R Hadi Farman. "The Healing Effect of Biodegradable Scaffolds Treated with Bone-Marrow Obtained Mesenchymal Stem Cells on Major Tendon Damage in the Dog as a Model". سامانه مدیریت نشریات علمی, 78, 3, 1402, 889-898. doi: 10.22092/ari.2022.359751.2470
Salman Hamza, A, Traef Ali, Q, Hadi Farman, R. (1402). 'The Healing Effect of Biodegradable Scaffolds Treated with Bone-Marrow Obtained Mesenchymal Stem Cells on Major Tendon Damage in the Dog as a Model', سامانه مدیریت نشریات علمی, 78(3), pp. 889-898. doi: 10.22092/ari.2022.359751.2470
Salman Hamza, A, Traef Ali, Q, Hadi Farman, R. The Healing Effect of Biodegradable Scaffolds Treated with Bone-Marrow Obtained Mesenchymal Stem Cells on Major Tendon Damage in the Dog as a Model. سامانه مدیریت نشریات علمی, 1402; 78(3): 889-898. doi: 10.22092/ari.2022.359751.2470

The Healing Effect of Biodegradable Scaffolds Treated with Bone-Marrow Obtained Mesenchymal Stem Cells on Major Tendon Damage in the Dog as a Model

Archives of Razi Institute
مقاله 14، دوره 78، شماره 3، مرداد و شهریور 2023، صفحه 889-898    اصل مقاله (951.98 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359751.2470
نویسندگان
A Salman Hamza؛ Q Traef Ali؛ R Hadi Farman
Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq
چکیده
The present study aimed to evaluate the implantation of decellularized small intestinal submucosa- extracellular matrix )SIS-ECM( seeded with bone marrow mesenchymal stem cells (BM-MSCs) to repair full-thickness Achilles tendon defect. For this purpose, 20 healthy adult stray dogs aged 8-12 months old (15±3 kg of weight) were enrolled in this study under an aseptic environment and general anesthesia. A 1.5 cm-long segment-based resection was performed in the mid-substance of the Achilles tendon in the control group (n=10) that did not receive treatment. While, in the experimental group (n=10), regarding the defect of the tendon, the stumps were bridged with decellularized SIS seeded with BM-MSCs (5×106) cells implanted. Afterward, the stumps of the tendon were sutured using the modified Kessler technique (4-0) polypropylene thread. The biomechanical observations of the tendon defect showed an increase in the tensile strength in the experimental group, compared to the control animals. It should be mentioned that this difference was significant (P≤0.05). Histopathological observations of biopsies harvested after the 4th, 8th, and 12th weeks revealed that the implanted graft had seeded with MSCs enhanced high-quality cellular infiltration and the host tissue healing was improved. Similar to the normal tendon, a dense organized collagenous tissue with high cellularity and vascularity was observed due to the presence of the remodeled ECM. However, the arrangement of collagen-fiber-derived connective tissue appeared to be more dominant than that in the experimental group, with less adhesion in the 12th week post-treatment. These findings suggest that the BM-MSCs inoculated with SIS can be employed to repair a damaged Achilles tendon due to the fact that this combination enhances the regeneration of the affected tendon.
کلیدواژه‌ها
Achilles tendon؛ Bone marrow؛ Dog؛ Implantation؛ Mesenchymal stem cells-Small intestinal submucosa (SIS)؛ Tendon-healing
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مراجع
  1. de Cássia Marqueti R, Almeida JA, Nakagaki WR, Guzzoni V, Boghi F, Renner A, et al. Resistance training minimizes the biomechanical effects of aging in three different rat tendons. J Biomech. 2017;53:29-35.
  2. Yang X, Meng H, Peng J, Xu L, Wang Y, Sun X, et al. Construction of microunits by adipose-derived mesenchymal stem cells laden with porous microcryogels for repairing an acute achilles tendon rupture in a rat model. Int J Nanomedicine. 2020;15:7155.
  3. Lantto I, Heikkinen J, Flinkkilä T, Ohtonen P, Leppilahti J. Epidemiology of A chilles tendon ruptures: Increasing incidence over a 33‐year period. Scand J Med Sci Sports. 2015;25(1):133-8.
  4. Aicale R, Tarantino D, Maffulli N. Overuse injuries in sport: a comprehensive overview. J Orthop Surg Res. 2018;13(1):1-11.
  5. Schäfer F-M, Stehr M. Tissue engineering in pediatric urology–a critical appraisal. Innov Surg Sci. 2018;3(2):107-18.
  6. Visser J, Gawlitta D, Benders KE, Toma SM, Pouran B, van Weeren PR, et al. Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles. Biomaterials. 2015;37:174-82.
  7. Qasim MT, Fenjan MN, Thijail HA. Molecular Identification of Cystoisospora Belli in Patients Infected With The Virus Human Immunodeficiency. Int J Drug Deliv Technol. 2022;12(2):701-4.
  8. Qasim M, Mahdi Mohammed Alakkam E, Mahdi Mohammed M, Hachim S, Sabah Jabr H, Emad Izzat S, et al. Ovine Pasteurellosis Vaccine: Assessment of the Protective Antibody Titer and Recognition of the Prevailing Serotypes. Arch Razi Inst. 2022;77(3):1207-10.
  9. Zadeh FA, Bokov DO, Salahdin OD, Abdelbasset WK, Jawad MA, Kadhim MM, et al. Cytotoxicity evaluation of environmentally friendly synthesis Copper/Zinc bimetallic nanoparticles on MCF-7 cancer cells. Rend Lincei Sci Fis Nat. 2022:1-7.
  10. Hafsan H, Bokov D, Abdelbasset WK, Kadhim MM, Suksatan W, Majdi HS, et al. Dietary Dracocephalum kotschyi essential oil improved growth, haematology, immunity and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss). Aquac Res. 2022;53(8):3164-75.
  11. Hernigou P, Flouzat Lachaniette CH, Delambre J, Zilber S, Duffiet P, Chevallier N, et al. Biologic augmentation of rotator cuff repair with mesenchymal stem cells during arthroscopy improves healing and prevents further tears: a case-controlled study. Int Orthop. 2014;38(9):1811-8.
  12. Butler DL, Juncosa‐Melvin N, Boivin GP, Galloway MT, Shearn JT, Gooch C, et al. Functional tissue engineering for tendon repair: A multidisciplinary strategy using mesenchymal stem cells, bioscaffolds, and mechanical stimulation. J Orthop Res. 2008;26(1):1-9.
  13. Tilley J, Chaudhury S, Hakimi O, Carr A, Czernuszka J. Tenocyte proliferation on collagen scaffolds protects against degradation and improves scaffold properties. J Mater Sci Mater Med. 2012;23(3):823-33.
  14. Kamishina H, Deng J, Oji T, Cheeseman JA, Clemmons RM. Expression of neural markers on bone marrow–derived canine mesenchymal stem cells. Am J Vet Res. 2006;67(11):1921-8.
  15. Hara S, Tanigawa T. Statistical Analysis System (SAS), SAS User's Guide: SAS/STAT Software Version 6 Statistical Analysis System (SAS), SAS User's Guide: SAS/STAT Software Version 6, 569-603, 1995. Anim Sci J. 2010;81(2):187-93.
  16. Gadupudi GS, Klaren WD, Olivier AK, Klingelhutz AJ, Robertson LW. PCB126-induced disruption in gluconeogenesis and fatty acid oxidation precedes fatty liver in male rats. Toxicol Sci. 2016;149(1):98-110.
  17. Ferrucci L, Fabbri E. Inflammageing: chronic inflammation in ageing, cardiovascular disease, and frailty. Nat Rev Cardiol. 2018;15(9):505-22.
  18. Sarrafian TL, Wang H, Hackett ES, Yao JQ, Shih M-S, Ramsay HL, et al. Comparison of Achilles tendon repair techniques in a sheep model using a cross-linked acellular porcine dermal patch and platelet-rich plasma fibrin matrix for augmentation. J Foot Ankle Surg. 2010;49(2):128-34.
  19. M Dohan Ehrenfest D, Bielecki T, Mishra A, Borzini P, Inchingolo F, Sammartino G, et al. In search of a consensus terminology in the field of platelet concentrates for surgical use: platelet-rich plasma (PRP), platelet-rich fibrin (PRF), fibrin gel polymerization and leukocytes. Curr Pharm Biotechnol. 2012;13(7):1131-7.
  20. Walden G, Liao X, Donell S, Raxworthy MJ, Riley GP, Saeed A. A clinical, biological, and biomaterials perspective into tendon injuries and regeneration. Tissue Eng Part B Rev. 2017;23(1):44-58.
  21. Müller SA, Dürselen L, Heisterbach P, Evans C, Majewski M. Effect of a simple collagen type I sponge for Achilles tendon repair in a rat model. Am J Sports Med. 2016;44(8):1998-2004.
  22. Meimandi-Parizi A, Oryan A, Moshiri A. Tendon tissue engineering and its role on healing of the experimentally induced large tendon defect model in rabbits: a comprehensive in vivo study. PloS One. 2013;8(9):e73016.
  23. Chae S, Sun Y, Choi Y-J, Ha D-H, Jeon I, Cho D-W. 3D cell-printing of tendon-bone interface using tissue-derived extracellular matrix bioinks for chronic rotator cuff repair. Biofabrication. 2021;13(3):035005.
  1. Moshiri A, Oryan A, Meimandiparizi A, Maffulli N. Collagen implants in experimental tendon injury in rabbits: a clinical, ultra-structural and biomechanical investigation. J Biol Regul Homeost Agents. 2014;28:381-97.
  2. Aamodt JM, Grainger DW. Extracellular matrix-based biomaterial scaffolds and the host response. Biomaterials. 2016;86:68-82.
  3. Docheva D, Müller SA, Majewski M, Evans CH. Biologics for tendon repair. Adv Drug Deliv Rev. 2015;84:222-39.
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