Jafari, O., Abdollahzadeh, E., Hassanzadeh Saber, M., Baradaran Noveiri, S. (2023). Cryopreservation of stellate sturgeon, Acipenser Stellatus spermatozoa using MT extender. , 7(7), 43-49.
Omid Jafari; Esmail Abdollahzadeh; Mohammad Hassanzadeh Saber; Shahrouz Baradaran Noveiri. "Cryopreservation of stellate sturgeon, Acipenser Stellatus spermatozoa using MT extender". , 7, 7, 2023, 43-49.
Jafari, O., Abdollahzadeh, E., Hassanzadeh Saber, M., Baradaran Noveiri, S. (2023). 'Cryopreservation of stellate sturgeon, Acipenser Stellatus spermatozoa using MT extender', , 7(7), pp. 43-49.
Jafari, O., Abdollahzadeh, E., Hassanzadeh Saber, M., Baradaran Noveiri, S. Cryopreservation of stellate sturgeon, Acipenser Stellatus spermatozoa using MT extender. , 2023; 7(7): 43-49.

Cryopreservation of stellate sturgeon, Acipenser Stellatus spermatozoa using MT extender

علوم آبزی پروری پیشرفته
Volume 7, Issue 7, March 2023, Pages 43-49    PDF (670.75 K)
Document Type: article
Authors
Omid Jafari* 1; Esmail Abdollahzadeh2; Mohammad Hassanzadeh Saber3; Shahrouz Baradaran Noveiri4
1Department of genetics and Biotechnology, International Sturgeon Research Institute, Rasht, Iran.
2International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Rasht, Iran
3AREEO
4International Sturgeon Research Institute
Abstract
Using cryopreservation techniques are so important in conducting brood stock selection programs, species protection, and accessibility to the spermatozoa throughout the year. Normally, in the conventional method in Iran, the formulation based on egg yolk and DMSO has been employed to freeze the sturgeon sperm. In the present study, the MT extender (Modified Tsvetkova’s) which consists of the main components of methanol, sucrose, potassium chloride, and Tris has been used for the preservation of Acipenser stellatus spermatozoa, without using chemical substance DMSO and egg yolk. Our results demonstrated that the frozen sperm had at least 30% motility after one year of storage in nitrogen tank. The results of this research show that this extender could be used to preserve Acipenser stellatus spermatozoa. Since the sperm’s concentration of sturgeon species are similar, the usage of the MT extender can be suggested for other species of the sturgeons.
Keywords
Acipenser stellatus; Cryopreservation; Extender; Methanol
References
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