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بررسی برخی از اسانسهای گیاهی بر روی رشد و تولید بیوفیلم باکتری‌های Pectobacterium carotovorum و Pectobacterium atrosepticum | ||
| مهار زیستی در گیاه‌پزشکی | ||
| دوره 9، شماره 2 - شماره پیاپی 18، اسفند 1400، صفحه 135-151 اصل مقاله (769.01 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2023.360551.324 | ||
| نویسندگان | ||
| میلاد آئینی* 1؛ حسین میرزایی نجفقلی2؛ فاطمه دریکوند3؛ میترا خادمی4؛ ندا روحانی5 | ||
| 1استادیار، گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| 2استادیار، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرم‌آباد، ایران | ||
| 3دانشجوی دکتری، گروه گیاه پزشکی ، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران | ||
| 4دانش آموخته دکتری، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان،خرم آباد، ایران | ||
| 5دانش آموخته دکتری، گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران | ||
| چکیده | ||
| این تحقیق با استفاده از برخی ترکیبات طبیعی برای مدیریت بیماری‌های پوسیدگی نرم باکتریایی با عامل sp. Pectobacterium انجام شد. اسانس گیاهان به‌لیمو (Lippia citriodora)، پونۀ کوهی (Origanum vulgare)، گوش بره (Phlomis fruticose)، مرزه (Satureja spicigera) و میخک (Syzygium aromaticum) با روش تقطیر با آب، استخراج و اثر بازدارندگی آنها در برابر بیمارگرهای باکتریایی عامل پوسیدگی نرم Pectobacterium carotovorum و Pectobacterium atrosepticum در شرایط آزمایشگاهی بررسی شد. اثرات ضدمیکروبی اسانسهای گیاهان دارویی به روش نشت در آگار نشان داد که تمامی اسانسهای مورد مطالعه دارای اثرات بازدارندگی معنیداری (P≤0.01) در جلوگیری از رشد بیمارگرهای باکتریایی بودند. اثر حداقل غلظت مهارکنندگی رشد (MIC) اسانسها بر تشکیل بیوفیلم دو عامل بیمارگر، نشان داد که در غلظت بالای MIC اسانس، یعنی در غلظت 125 پی‌پی‌ام بیوفیلم تشکیل نشده ، اما در غلظت‌های کمتر مثل 62/15 پی پی ام، بیوفیلم تشکیل شد. محاسبه غلظت بازدارنده افتراقی (شاخص (FIC حاکی از این بود که ترکیبهای اسانس مرزه با به‌لیمو علیه باکتری P. carotovorum فاقد اثر بودند. تجزیه و تحلیل عناصر سازندۀ اسانسها با کروماتوگرافی گازی–طیف‌سنجی جرمی (GC–MS)نشان داد که سیس–توجون (47/17%)، ایزوژرانیول (23%)، سیس–پیپریتنون (5/29%)، کارواکرول (5/88%) و اوژنول (7/98%) به ترتیب ترکیبات فعال اصلی در اسانسهای فرار گوش بره، به‌لیمو، پونۀ کوهی، مرزه و میخک بودند. نتیجه این که اسانسهای مورد مطالعه دارای اثرات ضدمیکروبی بر باکتری‌های مذکور بوده و از بین اسانسهای مورد بررسی اسانسهای به‌لیمو و گوش بره قوی‌تر بودند. براساس تحقیقات حاضر به نظر می‌رسد مادۀ مؤثره این دو اسانس در کنترل بیماری باکتریایی مورد نظر قابل استفاده باشد. همچنین توصیه می‌شود تاثیر این دو اسانس برای کنترل باکتری های بیماری‌زای دیگر بررسی گردد. | ||
| کلیدواژه‌ها | ||
| گیاهان دارویی؛ بازدارندگی؛ به‌لیمو؛ پونۀ کوهی؛ گوش بره؛ مرزه؛ میخک | ||
| عنوان مقاله [English] | ||
| Effects of some essential oils against growth and biofilm production of two plant pathogenic bacteria, Pectobaterium carotovorum and Pectobacterium atrosepticum | ||
| نویسندگان [English] | ||
| Milad Aeini1؛ Hossein Mirzaei Najafgholi2؛ Fatemeh Derikvand3؛ Mitra Khademi4؛ Neda Rouhani5 | ||
| 1Plant protection department, Faculty of agriculrure, Shahid Chamran university of Ahvaz | ||
| 2Assistant Professor, Plant Protection Department, Faculty of Agriculture, Lorestan university, Khorram Abad, Iran | ||
| 3Plant Protection Department, Faculty of Agriculture, Lorestan university, Khorram Abad, Iran | ||
| 4Production engineering and plant genetics department, Faculty of Agriculture, Lorestan university, Khorram Abad, Iran | ||
| 5Plant Protection department, Faculty of Agriculture, Lorestan university, Khorram Abad, Iran | ||
| چکیده [English] | ||
| Plant–derived essential oils (EOs) are a major group of environments–friendly compounds with antibacterial properties that have been used during the last decade. This study aimed to identify the chemical composition and evaluate the antimicrobial activity of EOs from Lippia citriodora, Origanum vulgare, Phlomis fruticose, Satureja spicigera, and Syzygium aromaticum against two soft rot– including bacteria, Pectobacterium carotovorum and Pectobacterium atrosepticum. Plants were collected from Khuzestan province, and their essential oil composition was determined from gas–chromatography–mass spectrometry (GC–MS) analysis. The antibacterial effect of Eos was measured by the disk diffusion method. The effect of EOs on biofilm formation was also assayed. In addition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were calculated accordingly. The interaction among the EOs was calculated according to the fractional inhibitory concentration (FIC) index using the Modified Dilution Checkboard method. The result showed that all Eos inhibited bacterial growth. The highest MIC (31/25) was attributed to P. fruticosa against P. carotovorum while the lowest MIC (125) belongs to L. citriodora and S. spicigera against P. carotovorum. FIC index showed that L. citriodora and S. spicigera are neutral to P. carotovorum. Analysis of GC–MS chromatogram indicated that the major constituents of P. fruticosa, L. citriodora, O. vulgare, S. spicigera, and S. aromaticum were cis–Thujone (17.47%), Isogeraniol (23%), cis Piperitone oxide (29.5%), Carvacrol (88.5%), Eugenol (98.7%), respectively. According to the results, in high MIC of Eos (125 ppm), bacteria were not able to form biofilm. Our results indicate that these five plant species have antimicrobial activity against Pectinbacterium species. Among all Eos used, L. citriodora and P. fruticose showed the highest activity which, therefore, can be recommended for further applications. | ||
| کلیدواژه‌ها [English] | ||
| Disk diffusion, medicinal plant, inhibition, pathogenesis | ||
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