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Study of humoral and cellular immunity and histopathology of target tissues following Newcastle Clone12IR vaccine administration in SPF chickens | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 11 تیر 1402 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2023.362500.2748 | ||
| نویسندگان | ||
| Mohammad Majid Ebrahimi* ؛ Shahla Shahsavandi؛ Mohammad Eslampanah؛ Ali Reza Yousefi | ||
| Razi Vaccine and Serum Research Institute | ||
| چکیده | ||
| Newcastle disease (ND) is a highly contagious viral infection affecting the poultry production in many countries. Strict biosecurity and administration of live attenuated vaccines against ND virus (NDV) are the main implements in the controlling programs. This study evaluated the efficacy and potency of the Razi Clone12IR Newcastle vaccine in specific pathogen-free (SPF) chickens. Chickens were vaccinated with either the Razi Clone12IR vaccine (group A1; n=20) or an imported Clone vaccine (B1; n=20) in the first week of life and boosted in the second weeks via eye drop, while negative control chickens received PBS (C1; n=20). Half of the birds in each group were challenged with the virulent NDV strain in the 3rd post-vaccination week (A2, B2, and C2 groups). Specific antibody response was determined on the collected sera by hemagglutination inhibition (HI) assay up to 8 weeks. Cell-mediated immunity (CMI) was determined by lymphocyte proliferation assay at 3 and 6 weeks after the second vaccination. Sections of the tissues and organs including trachea, lung, cecal tonsile, spleen, bursa of Fabricius, liver, and small intestine were subjected to histopathology. The immunized groups A1 and B1 showed significantly higher HI antibody titers before challenge compared to the control group. The lymphocyte proliferation response in the peripheral blood of the vaccinated groups was significantly increased. After challenge, A2 and B2 groups conferred good protection and drastically reduced virus shedding. No main lesions were noted in the vaccinated group's tissues or organs in histopathology. In a few cases, mild microscopic lesions including the infiltration of inflammatory cells, which were related to the effect of the vaccine virus, were observed. These results indicate that the Razi Clone12IR vaccine is safe and can be an efficient tool for NDV infections by inducing protective humoral and CMI responses. | ||
| کلیدواژه‌ها | ||
| Clone 12IR strain؛ Histopathology؛ Immune responses؛ Newcastle disease vaccine؛ Protection | ||
| عنوان مقاله [English] | ||
| - | ||
| چکیده [English] | ||
| - | ||
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آمار تعداد مشاهده مقاله: 36 |
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