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فعالیت ضد قارچی Beauveria bassiana در برابر قارچ عامل خشکیدگی شمشاد خزری (Calonectria pseudonaviculata) | ||
| مهار زیستی در گیاه‌پزشکی | ||
| مقاله 7، دوره 10، شماره 1، شهریور 1401، صفحه 127-142 اصل مقاله (977.71 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2023.362305.333 | ||
| نویسندگان | ||
| سیده معصومه زمانی* 1؛ سمیرا فراهانی2؛ ابراهیم زرقانی3؛ نرگس سپاسی4 | ||
| 1استادیار پژوهش، موسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 2استادیار پژوهش، مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران. | ||
| 3استادیار،باغ گیاه شناسی نوشهر، موسسه تحقیقات جنگل ها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی،نوشهر، ا یران | ||
| 4پژوهشگر، مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| چکیده | ||
| شمشاد خزری از ارزشمندترین درختان پهن‌برگ همیشه‌سبز جنگل‌های هیرکانی است و متاسفانه در دهه اخیر به بیماری بلایت ناشی از قارچ Cylindrocladium buxicola مبتلا شده است که خطر جدی برای بقا و تولید شمشاد می‌باشد. با توجه به وجود درختان شمشاد در اکوسیستم‌های جنگل و فضای سبز مناطق شهری، باید از ترکیبات ایمن و دوستدار محیط‌زیست برای کنترل بیماری استفاده شود. Beauveria bassiana قارچ اندوفیت گیاهی با توانایی بیماریزایی در حشرات است که خواص آن در افزایش رشد گیاهان، کنترل زیستی آفات و محدود کردن فعالیت قارچ‌های بیماری‌زای گیاهی در چندین گیاه میزبان ثابت شده است. هدف از این مطالعه بررسی پتانسیل برخی از جدایه‌های B. bassiana بدست آمده از آفت برگخوار شمشاد (Cydalima perspectalis) در کنترل قارچ C. buxicola بود. فعالیت آنتاگونیستی چهار جدایه از B. bassiana با استفاده از کشت دوگانه در شرایط آزمایشگاهی و همچنین گیاهان با تلقیح قارچ اندوفیت در بذور گیاهان و تلقیح قارچ پاتوژن در برگ‌ها ارزیابی شد. هر چهار جدایه قادر به برقراری ارتباط اندوفیتی روی گیاهچه‌های شمشاد بودند. همه جدایه‌ها در شرایط آزمایشگاهی فعالیت ضدقارچی قابل توجهی در برابر C. buxicola (53–88%) نشان دادند. در نتیجه تلقیح قارچ اندوفیت به گیاه، اثر حفاظتی بالایی در برابر قارچ بلایت مشاهده شد و درصد سطح خسارت برگی در گیاهان شمشاد تلقیح شده با قارچ‌های اندوفیت به‌طور معنی‌داری کمتر از گیاهانی بود که فقط توسط پاتوژن تلقیح شده بودند. نتایج این تحقیق مسیر را برای استفاده موثرتر از B. bassiana در مدیریت تلفیقی و همزمان آفت برگخوار شمشاد خزری و قارچ عامل بلایت این گیاهان و همچنین افزایش رشد گیاه میزبان هموار می‌کند. | ||
| کلیدواژه‌ها | ||
| قارچ‌های بیمارگر حشرات؛ کنترل بیولوژیک؛ میکروارگانیسم‌های مفید؛ فعالیت ضد قارچی؛ Cylindrocladium buxicola؛ Cydalima perspectalis | ||
| عنوان مقاله [English] | ||
| Antifungal Activity of Beauveria bassiana Endophyte against Boxwood Blight Pathogen (Calonectria pseudonaviculata) | ||
| نویسندگان [English] | ||
| Seyedeh Masoomeh Zamani1؛ Samira Farahani2؛ Ebrahim Zarghani3؛ Narges Sepasi4 | ||
| 1Assistant Prof., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO),Tehran, Iran | ||
| 2Assistant Prof., Research Institute of Forests and Rangeland, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran. | ||
| 3Botanical Garden of Noshahr, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Nowshahr, Iran | ||
| 4Research Institute of Forests and Rangeland, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran. | ||
| چکیده [English] | ||
| Caspian boxwood is one of the most valuable broadleaf evergreen trees in Hyrcanian forests and unfortunately, in the last decade, it has been affected by blight disease caused by the fungus Cylindrocladium buxicola, which is a serious threat to the survival and production of boxwood. Due to the presence of boxwood trees in the forest ecosystems and green spaces of urban areas, safe and environmentally friendly compounds should be used to control the disease. Beauveria bassiana is a plant endophytic fungus with pathogenic ability in insects, whose properties have been proven to increase plant growth, biocontrol pests and limit the activity of phytopathogenic fungi in several host plants. The aim of this study was to investigate the potential of some B. bassiana isolates obtained from the Cydalima perspectalis in controlling the fungus C. buxicola. Antagonistic activity of four isolates of B. bassiana was evaluated using double culture in laboratory conditions and also in the plants by inoculation of endophyte fungus in seeds of plants and inoculation of pathogen fungus in leaves. All four isolates were able to establish endophytic relationship on boxwood seedlings. All isolates showed significant antifungal activity against C. buxicola (53–88%) in vitro. As a result of inoculating the plant with endophytic fungi, a high protective effect against blight fungus was observed and the percentage of leaf damage in boxwood plants inoculated with endophytic fungi was significantly lower than the plants that were inoculated only by the pathogen. The results of this research pave the way for a more effective use of B. bassiana in the integrated and simultaneous management of the leaf–eating pest of Caspian boxwood and the blight fungus of these plants, as well as increasing the growth of the host plant. . | ||
| کلیدواژه‌ها [English] | ||
| Insect pathogenic fungi, biological control, beneficial microorganisms, antifungal activity, Cylindrocladium buxicola, Cydalima perspectalis | ||
| مراجع | ||
|
Akutse, K.S., Fiaboe, K.K.M., Van Den Berg, J., Ekesi, S. & Maniania, N.K. 2014. Effects of endophyte colonization of Vicia faba (Fabaceae) plants on the life–history of leafminer parasitoids Phaedrotoma scabriventris (Hymenoptera: Braconidae) and Diglyphus isaea (Hymenoptera: Eulophidae). PLOS One, 9: 1–11. Aly, A.H., Debbab, A., Kjer, J. & Proksch, P. 2010. Fungal endophytes from higher plants: A prolific source of phytochemicals and other bioactive natural products. Fungal Diversity, 41: 1–16. Anees, M., Tronsmo, A., Edel–Hermann, V., Hjeljord, L.G., Héraud, C. & Steinberg, C. 2010. Characterization of field isolates of Trichoderma antagonistic against Rhizoctonia solani. Fungal Biology, 114: 691–701. Arnold, A.E. & Lewis, L.C. 2005. Ecology and Evolution of Fungal Endophytes, and Their Roles against Insects. Insect–Fungal Associations: Ecology and Evolution, Oxford University Press: New York, NY, USA, 74–96. Bailey, K.L., Boyetchko, S.M. & Langle, T. 2010. Social and economic drivers shaping the future of biological control: A Canadian perspective on the factors affecting the development and use of microbial biopesticides. Biological Control, 52: 221–229. Barelli, L., Moonjely, S., Behie, S.W. & Bidochka, M.J. 2016. Fungi with multifunctional lifestyles: Endophytic insectpathogenic fungi. Plant Molecular Biology, 90: 657–664. Bark, Y.G., Lee, D.G., Kang, S.C., Kim, Y.H. 1996. Antibiotic properties of an entomopathogenic fungus, Beauveria bassiana, on Fusarium oxysporum and Botrytis cinerea. Korean Journal of Plant Pathology, 12: 245–250. Barnett, H.L. & Hunter, B.B. 1998. Illustrated genera of imperfect fungi. 3a. ed. American Phytopathological Society, Saint Paul, Minnesota, U.S.A., 240 p. Barra–Bucarei, L., France, A. & Millas, P. 2019. Crossing frontiers: Endophytic entomopathogenic fungi for biological control of plant diseases. In Endophytes for a Growing World, Cambridge University Press: Cambridge, UK, p. 67. Barra–Bucarei, L. & Gerding, M. 2020. Antifungal Activity of Beauveria bassiana Endophyte against Botrytis cinerea in Two SolanaceaeCrops. Microorganisms, 8: 65. Behie, S.W., Jones, S.J. & Bidochka, M.J. 2015. Plant tissue localization of the endophytic insect pathogenic fungi Metarhizium and Beauveria. Fungal Ecology, 13: 112–119. Behie, S.W., Zelisko, P.M. & Bidochka, M.J. 2012. Endophytic insect–parasitic fungi translocate nitrogen directly from insects to plants. Science, 336: 1576–1577. Card, S., Johnson, L., Teasdale, S. & Caradus, J. 2016. Deciphering endophyte behaviour: The link between endophyte biology and efficacious biological control agents. FEMS Microbiology Ecology, 92. Crous, P.W. 2002. Taxonomy and pathology of Cylindrocladium (Calonectria) and allied genera. APS Press, St. Paul, Minnesota, U.S.A. Culebro–Ricaldi, J.M., Ruiz–Valdiviezo, V.M., Rodrguez–Mendiola, M.A., avila–Miranda, M.E., Gutiérrez– Miceli, F.A., CruzRodriguez, R.I., Dendooven, L. & Montes–Molina, J.A. 2017. Antifungal properties of Beauveria bassiana strains against Fusarium oxysporum f. sp. lycopersici race 3 in tomato crop. Journal of Environmental Biology, 38: 821–827. Dara, S.K. 2019. Non–entomopathogenic roles of entomopathogenic fungi in promoting plant health and growth. Insects, 10: 277p. El Kichaoui, A., Elnabris, K., Shafie, A., Fayyad, N., Arafa, M. & El Hindi, M. 2017. Development of Beauveria bassiana–Based Bio–Fungicide Against FusariumWilt Pathogens for Capsicum annuum, a Promising Approach Toward Vital Biocontrol Industry in Gaza Strip. IUG Journal of Natural Studies, 25: 183–190. Gathage, J.W., Lagat, Z.O., Fiaboe, K.K.M., Akutse, K.S., Ekesi, S. & Maniania, N.K. 2016. Prospects of fungal endophytes in the control of Liriomyza leafminer flies in common bean Phaseolus vulgaris under field conditions. BioControl, 61: 741–753. Glare, T., Caradus, J., Gelernter, W., Jackson, T., Keyhani, N., Köhl, J., Marrone, P., Morin, L. & Stewart, A. 2012. Have Biopesticides come of age? Trends Biotechnology, 30: 250–258. Gonzalez–Mas, N., Cuenca–Medina, M., Gutiérrez–Snchez, F. & QuesadaMoraga, E. 2019a. Bottom–up effects of endophytic Beauveria bassiana on multitrophic interactions between the cotton aphid, Aphis gossypii, and its natural enemies in melon. Journal of Pest Science, 92: 1271–1281. Gonzalez–Mas, N., Sanchez–Ortiz, A., Valverde–Garcca, P., QuesadaMoraga, E. 2019b. Effects of endophytic entomopathogenic ascomycetes on the life–history traits of Aphis gossypii Glover. Insects, 10: 165. Greenfield M., Gómez–Jiménez M.I., Ortiz V., Vega F.E., Kramer M. & Parsa S. 2016. Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation. Biological Control, 95: 40–48. Henricot, B. & Culham, A., 2002. Cylindrocladium buxicola, a new species affecting Buxus spp, and its phylogenetic status. Mycologia, 94(6): 980–997. Henricot, B., Pérez Sierra, A. & Prior, C., 2000. A new blight disease on Buxus in the UK caused by the fungus Cylindrocladium. Plant Pathology, 49: 805 p. Herre, E.A., Mejia, L.C., Kyllo, D.A., Rojas, E., Maynard, Z., Butler, A. & Van Bael, S.A. 2007. Ecological implications of anti–pathogen e_ects of tropical fungal endophytes and mycorrhizae. Ecology, 88: 550–558. Humber, R.A. 1997. Fungi: Identification. In L.A. Lacey (ed.) Manual of Techniques in Insect Pathology. Academic Press. New York, USA, 153– 185. Jaber, L.R. & Araj, S.E. 2018. Interactions among endophytic fungal entomopathogens (Ascomycota: Hypocreales), the green peach aphid Myzus persicae Sulzer (Homoptera: Aphididae), and the aphid endoparasitoid Aphidius colemani Viereck (Hymenoptera: Braconidae). Biological Control, 116: 53–61. Jaber, L.R. & Enkerli, J. 2016. Effect of seed treatment duration on growth and colonization of Vicia faba by endophytic Beauveria bassiana and Metarhizium brunneum. Biological Control, 103: 187–195 Jaber, L.R. & Ownley, B.H. 2018. Can we use entomopathogenic fungi as endophytes for dual biological control of insect pests and plant pathogens? Biological Control, 116: 36–45. Jaber, L.R. & Salem, N.M. 2014. Endophytic colonisation of squash by the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales) for managing Zucchini yellow mosaic virus in cucurbits. Biocontrol Science and Technology, 24: 1096–1109. Jalili, A. & Jamzad, Z. 1999. Red Data Book of Iran: A Preliminary Survey of Endemic, Rare and Endangered Plant Species in Iran. Research Institute of Forests and Rangelands Publications, Tehran, 748p. Kauppinen, M., Saikkonen, K., Helander, M., Pirttilä, A.M. & Wäli, P.R. 2016. Epichloë grass endophytes in sustainable agriculture. Nature Plants, 2: 15224. Khare, E., Mishra, J. & Arora, N.K. 2018. Multifaceted interactions between endophytes and plant: developments and Prospects. Front. Microbiology, 9: 2732. Kim, J.–C., Choi, G.J., Park, J.–H., Kim, H.T. & Cho, K.Y. 2001. Activity against plant pathogenic fungi of phomalactone isolated from Nigrospora sphaerica. Pest Manag. Sci. Former. Journal of Pesticide Science, 57: 554–559. Lareen, A., Burton, F. & Schäfer, P. 2016. Plant root–microbe communication in shaping root microbiomes. Plant Molecular Biology, 90: 575–587. Lobo, C. B., Tomás, M. S. J., Viruel, E., Ferrero, M. A. & Lucca, M. E. 2019. Development of low–cost formulations of plant growth–promoting bacteria to be used as inoculants in beneficial agricultural technologies. Microbiological Research, 219: 12–25. Lozano–Tovar, M.D., Garrido–Jurado, I., Quesada–Moraga, E., Raya–Ortega, M.C. & Trapero–Casas, A. 2017. Metarhizium brunneum and Beauveria Bassiana release secondary metabolites with antagonistic activity against Verticillium Dahliae and Phytophthora Megasperma olive pathogens. Crop Protection, 100: 186–195. Lozano–Tovar, M.D., Ortiz–Urquiza, A., Garrido–Jurado, I., Trapero–Casas, A. & Quesada–Moraga, E. 2013. Assessment of entomopathogenic fungi and their extracts against a soil–dwelling pest and soil–borne pathogens of olive. Biol.ogical Control, 67: 409–420. Martin–Hernandez, A.M., Dufresne, M., Hugouvieux, V., Melton, R. & Osbourn, A. 2000. Effects of targeted replacement of the tomatinase gene on the interaction of Septoria lycopersici with tomato plants. Molecular Plant–Microbe Interactions, 13: 1301–1311. Mirabolfathy, M., Ahangaran, Y., Lombard, L. & Crous, P.W. 2013. Leaf blight of Buxus sempervirens in northern forests of Iran caused by Calonectria pseudonaviculata, Plant Disease, 97(8): 1121 p. Moore, D., Bateman, R.P., Carey, M. & Prior, C. 1995. Long–term storage of Metarhizium flavoviride conidia in oil formulations for the control of locusts and grasshoppers. Biocontrol Science and Technology, 5: 193–200. Nagaoka, T., Nakata, K. & Kouno, K. 2004. Antifungal activity of oosporein from an antagonistic fungus against Phytophthora infestans. Zeitschrift für Naturforschung C., 59: 302–304. Orole, O.O. & Adejumo, T.O. 2009. Activity of fungal endophytes against four maize wilt pathogens. African Journal of Microbiology Research, 3: 969–973. Ownley, B.H., Griffin, M.R., Klingeman, W.E., Gwinn, K.D., Moulton, J.K. & Pereira, R.M. 2008. Beauveria bassiana: endophytic colonization and plant disease control. Journal of Invertebrate Pathology, 3: 267–270 Ownley, B.H., Gwinn, K.D. & Vega, F.E. 2010. Endophytic fungal entomopathogens with activity against plant pathogens: Ecology and evolution. In The Ecology of Fungal Entomopathogens, Springer: Dordrecht, The Netherlands, ISBN 9789048139651. Ownley, B.H., Pereira, R.M., Klingeman, W.E., Quigley, N.B. & Leckie, B.M. 2004. Beauveria bassiana, a dual-purpose biological control with activity against insect pests and plant pathogens. Emerg. Concepts Plant Health Manag, 255–269. Quesada–Moraga, E., Landa, B.B., Muaoz–Ledesma, J., Jiménez–Dnaz, R.M. & Santiago–alvarez, C .2006. Endophytic colonization of opium poppy, Papaver somniferum, by an entomopathogenic Beauveria bassiana strain. Mycopathologia, 161: 323–329 Quesada–Moraga, E., Munoz–Ledesma, F.J. & Santiago–Alvarez, C. 2009. Systemic protection of Papaver somniferum L. against Iraella luteipes (Hymenoptera: Cynipidae) by an endophytic strain of Beauveria bassiana (Ascomycota: Hypocreales). Environmental Entomology, 38: 723–730. Renwick, A., Campbell, R. & Coe, S. 1991. Assessment of in vivo screening systems for potential biocontrol agents of Gaeumannomyces graminis. Plant Pathology, 40: 524–532. Sinno, M., Ranesi, M., Di, Lelio, I., Iacomino, G., Becchimanzi, A., Barra, E., Molisso, D., Pennacchio, F., Digilio, M.C., Vitale, S., Turrà, D., Harizanova, V., Lorito, M. & Woo, S.L. 2021. Selection of Endophytic Beauveria bassiana as a Dual Biocontrol Agent of Tomato Pathogens and Pests. Pathogens, 10(10): 1242. Sword, G.A., Tessnow, A. & Ek–Ramos, M. J. 2017. Endophytic fungi alter sucking bug responses to cotton reproductive structures. Insect Science, 24: 1003–1014. Tefera, T., Vidal, S. 2009. Effect of Inoculation Method and Plant Growth Medium on Endophytic Colonization of Sorghum by the Entomopathogenic Fungus Beauveria bassiana. Biocontrol, 54: 663–669. Vega, F. E. 2018. The use of fungal entomopathogens as endophytes in biological control: a review. Mycologia 110, 4–30. doi: 10.1080/00275514.2017.1418578 Vega, F.E., Goettel, M.S., Chandler, D., Jackson, M.A., Keller, S., Koike, M., Maniania, N.K., Monzon, A., Ownley, B.H., Pell, J.K., et al. 2009. Fungal entomopathogens: new insights on their ecology. Fungal Ecol. 2: 149–159. Wagner, B.L. & Lewis, L.C. 2000. Colonization of corn, Zea mays, by the entomopathogenic fungus Beauveria bassiana. Applied and Environmental Microbiology, 66: 3468–3473. Wang, Q. & Xu, L. 2012. Beauvericin, a bioactive compound produced by fungi: A short review. Molecules, 17: 2367–2377. Watanabe, T. 2010. Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species, CRC press: Boca Raton, FL, USA. Woo, S.L. & Pepe, O. 2018. Microbial consortia: Promising probiotics as plant biostimulants for sustainable agriculture. Front. Plant Science, 9: 7–12. Wraight, S.P., Carruthers, R.I., Jaronski, S.T., Bradley, C.A., Garza, C.J. & Galaini–Wraight, S. 2000. Evaluation of the entomopathogenic fungi Beauveria bassiana and Paecilomyces fumosoroseus for microbial control of the silverleaf whitefly, Bemisia argentifolii. Biological Control, 17: 203–217. Zamani, S.M., Gholami Ghavamabad, R. & Kazerani, F. 2023. Efficacy of indigenous isolates of entomopathogenic fungi, Beauveria bassiana against the box tree moth, Cydalima perspectalis, invasive pest in Iranian forests. Bulletin of Insectology, 76(1): 117–125. | ||
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