Farjami, A., Soleimanzadeh, A., Ayen, E. (2024). Evaluation of co-supplementation of Rutin in Simmental bull semen extender: Evaluation of kinetic parameters, sperm quality. , 37(1), 79-88. doi: 10.22092/vj.2023.362454.2072
Amir Farjami; Ali Soleimanzadeh; Esmail Ayen. "Evaluation of co-supplementation of Rutin in Simmental bull semen extender: Evaluation of kinetic parameters, sperm quality". , 37, 1, 2024, 79-88. doi: 10.22092/vj.2023.362454.2072
Farjami, A., Soleimanzadeh, A., Ayen, E. (2024). 'Evaluation of co-supplementation of Rutin in Simmental bull semen extender: Evaluation of kinetic parameters, sperm quality', , 37(1), pp. 79-88. doi: 10.22092/vj.2023.362454.2072
Farjami, A., Soleimanzadeh, A., Ayen, E. Evaluation of co-supplementation of Rutin in Simmental bull semen extender: Evaluation of kinetic parameters, sperm quality. , 2024; 37(1): 79-88. doi: 10.22092/vj.2023.362454.2072

Evaluation of co-supplementation of Rutin in Simmental bull semen extender: Evaluation of kinetic parameters, sperm quality

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 10, Volume 37, Issue 1, March 2024, Pages 79-88    PDF (903.34 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2023.362454.2072
Authors
Amir Farjami1; Ali Soleimanzadeh* 2; Esmail Ayen1
1Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
2Associate Professor, Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University
Abstract
This study investigates the effects of cryopreservation and supplementation of Simmental bull’s semen with Rutin. Therefore, this study aimed to assess motility parameters, sperm viability, oxidative stress parameters, and DNA damage to detect the optimum concentrations of Rutin for Simmental bull semen cryopreservation. Five concentrations of Rutin with 0.2, 0.4, 0.6, 0.8, and 1.0 mM were added to the freezing extender of Simmental bull sperm, respectively. The obtained results showed that the addition of 0.4 and 0.6 mM of rutin significantly improves total motility, progressive motility, plasma membrane integrity, total antioxidant capacity, reducing damage to sperm DNA, sperm viability and motility characteristics, except STR, compared to the control group. In conclusion, supplementation with Rutin in cryopreservation medium protects Simmental bull sperm against ROS attack by enhancing the antioxidative defense. Therefore, we conclude that the addition of 0.4 and 0.6 mM rutin to the Simmental bovine sperm cryopreservation extender can improve post-freezing sperm quality and may improve conception rates after artificial insemination with frozen sperm.
Keywords
Rutin; kinetic parameters; sperm quality; Simmental bull
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