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بررسی تاثیر پیش‌تیمار اسید سالیسیلیک بر جوانه‌زنی بذرهای زوال یافته و آنزیم‌های آنتی‌اکسیدانی گیاه کدوی پوست کاغذی تحت تنش خشکی | ||
| علوم و فناوری بذر ایران | ||
| مقاله 3، دوره 13، شماره 2 - شماره پیاپی 34، تیر 1403، صفحه 35-49 اصل مقاله (586.49 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2023.362518.1491 | ||
| نویسنده | ||
| نساء قره باغلی* | ||
| استادیار، بخش تحقیقات گیاهان زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع‌طبیعی استان همدان، سازمان تحقیقات، آموزش و ترویج کشاورزی، همدان، ایران. | ||
| چکیده | ||
| با هدف ارزیابی اثر اسید سالیسیلیک در بهبود کارآیی بذرهای زوال یافته کدوی پوست کاغذی در شرایط تنش خشکی، آزمایشی به شکل فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار به اجرا درآمد. پیش‌تیمار غلظت‌های مختلف اسید سالیسیلیک شامل صفر (پیش تیمار شده با آب)، 1 و 5/1 میلی‌مولار در سطوح مختلف خشکی صفر، 2/0-، 4/0- و 6/0- مگاپاسکال مورد بررسی قرار گرفت. شاخص های متوسط زمان جوانه‌زنی، درصد جوانه‌زنی، طول گیاهچه، بنیه بذر، نشت الکترولیتی غشاء، قندهای محلول، پروتئین‌های محلول، مالون دی‌آلدهید، فعالیت آسکوربات پراکسیداز، سوپراکسید دیسموتاز و آنزیم‌های کاتالاز مورد ارزیابی قرار گرفتند. نتایج نشان داد پیش‌تیمار بذرها با غلظت‌های مختلف اسید سالیسیلیک، از کاهش معنی‌دار شاخص‌های جوانه‌زنی بذرهای زوال یافته کدوی پوست کاغذی تحت تنش خشکی جلوگیری نمود. به‌طوری‌که در پتانسیل 6/0- مگاپاسکال، پیش‌تیمار بذرها با 5/1 میلی‌مولار اسید سالیسیلیک درصد جوانه‌زنی، شاخص بنیه بذر، قندها و پروتئین‌های محلول را به‌ترتیب 2/80، 4/169، 6/51، 4/93 درصد و برای فعالیت آسکوربات پراکسیداز، سوپراکسید دیسموتاز و آنزیم‌های کاتالاز را به‌ترتیب 2/41، 12 و 8/11 درصد نسبت به شاهد افزایش داد. بر اساس یافته‌های تحقیق پیش‌تیمار اسید سالیسیلیک با غلظت 5/1 میلی‌مولار به منظور کاهش تنش اکسیداتیو ناشی از خشکی برای بذر های زوال یافته کدوی پوست کاغذی توصیه می‌شود. | ||
| کلیدواژه‌ها | ||
| اسید سالیسیلیک؛ پروتئین‌های محلول؛ تنش اکسیداتیو؛ درصد جوانه‌زنی | ||
| عنوان مقاله [English] | ||
| Investigating the effect of pretreatment of salicylic acid on the germination of aged pumpkin (Cucurbita pepo L.) seeds and antioxidant enzymes of plant under drought stress | ||
| نویسندگان [English] | ||
| Nesa Gharehbaghli | ||
| Assistant professor, Horticulture Crops Research Department, Hamedan Agriculture and Natural Resources Research and Education Center (AREEO), Hamedan, Iran. | ||
| چکیده [English] | ||
| With the aim of evaluating the effect of salicylic acid in improving the efficiency of the aged pumpkin seeds under drought stress conditions, a factorial experiment was carried out in the form of a completely randomized design with three replications. Pretreatment of different concentrations of salicylic acid including zero (pretreated with water), 1 and 1.5 mM at different drought levels of zero, -0.2, -0.4 and -0.6 MPa were investigated. Indicators of mean germination time, germination percentage, seedling length, seed vigor, membrane electrolyte leakage, soluble carbohydrates, soluble proteins, malondialdehyde, ascorbate peroxidase activity, superoxide dismutase and catalase enzymes were evaluated. The results showed that the pretreatment of seeds with different concentrations of salicylic acid prevented the significant decrease in the germination indices of the deteriorated pumpkin seeds under drought stress. So that at the potential of -0.6 MPa, the treatment of seeds with 1.5 mM salicylic acid increased the percentage of germination, seed germination index, soluble carbohydrates and soluble proteins respectively 80.2, 169.4, 51.6, 93.4% and for the activity of ascorbate peroxidase, superoxide dismutase and catalase enzymes, 41.2%, 12% and 11.8%, respectively, compared to the control. Based on the research findings, pretreatment of salicylic acid (1.5 mM) is recommended in order to reduce the oxidative stress caused by drought for pumpkin. | ||
| کلیدواژه‌ها [English] | ||
| germination percentage, oxidative stress, salicylic acid, soluble proteins | ||
| مراجع | ||
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