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بررسی اثر پوترِسین و تنش شوری بر برخی از شاخص‌های جوانه‌زنی بذور حنا (توده بومی جنوب کرمان) | ||
| علوم و فناوری بذر ایران | ||
| مقاله 3، دوره 13، شماره 3 - شماره پیاپی 35، مهر 1403، صفحه 39-52 اصل مقاله (669.7 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2023.362439.1485 | ||
| نویسنده | ||
| ابوالقاسم حمیدی مقدم* | ||
| گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه جیرفت، جیرفت، ایران. | ||
| چکیده | ||
| حنا (Lawsonia inermis L.) درختچه‌ای زینتی و دارویی، حاوی ماده موثره لاوسون بوده که در برخی از مناطق جنوب و جنوب شرق ایران کشت می‌شود. تنش شوری یکی از عمده‌ترین تنش‌های محیطی غیر زیستی است که تمام مراحل رشد و نمو گیاه را تحت تاثیر قرار می‌دهد. پلی‌آمین‌ها از جمله پوترسین می‌توانند نقش مهمی در رشد و نمو گیاه و پاسخ به تنش ایفا کنند. به منظور بررسی تاثیر پوترسین و تنش شوری NaCl بر برخی از شاخص‌های جوانه‌زنی، یک آزمایش فاکتوریل و در قالب طرح کاملاً تصادفی در چهار تکرار در ژرمیناتور به مدت 11 روز اجرا شد. فاکتور اول سطوح پوترسین (صفر و 75/0 میلی‌مولار) و فاکتور دوم شامل چهار سطح تنش شوری (صفر، 50، 100 و 150 میلی‌مولار) بود. نتایج نشان داد میان تیمارهای پوترسین اختلاف معنی‌داری در سطح شوری شاهد بر شاخص‌های جوانه‌زنی بذور حنا به غیر از شاخص بنیه بذر وجود ندارد. اگرچه برهمکنش پوترسین در شوری سبب کاهش آثار منفی تنش شوری 50 و 100 میلی‌مولار بر سرعت جوانه‌زنی شد، ولی سبب تشدید آثار منفی تنش شوری 150 میلی‌مولار بر درصد جوانه‌زنی، طول ریشه‌چه، طول ساقه‌چه، وزن گیاهچه، شاخص بنیه بذر و فعالیت آلفاآمیلاز در مقابسه با شاهد شد. نتایج نشان داد که جوانه‌زنی بذور حنا حساس به تنش شوری است و تنها قادر به تحمل سطوح پایین تنش شوری (50 میلی‌مولار) می‌باشند. با توجه به همبستگی مثبت و معنی‌داری بین فعالیت آنزیم آلفاآمیلاز و درصد جوانه‌زنی (r=0.98)، می‌توان استنباط کرد که احتمالاً تغییر در فعالیت آنزیم آلفاآمیلاز سبب کاهش درصد جوانه‌زنی شده است. | ||
| کلیدواژه‌ها | ||
| بنیه بذر؛ پلی‌آمین؛ تحمل به شوری؛ درصد جوانه‌زنی | ||
| عنوان مقاله [English] | ||
| Investigating the effect of putrescine and salinity stress on some germination indices of Henna seeds (South Kerman's native) | ||
| نویسندگان [English] | ||
| Abolghasem Hamidi Moghaddam | ||
| Department of Horticultural Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran. | ||
| چکیده [English] | ||
| Henna (Lawsonia inermis L.) is an ornamental and medicinal plant containing Lawson’s active substance, which is cultivated in some regions of the south and southeast of Iran. Salinity is one of the major abiotic environmental stress that affects almost all stages of plant development. Polyamines such as putrescine can play important roles in plant growth and stress responses. In order to investigate the effect of putrescine and NaCl salinity stress on some germination indices, a factorial experiment was conducted with two levels of putrescine (0 and 0.75 mM) and four salinity levels (0, 50, 100 and 150 mM) based on completely randomized design with four replications in germinator for 11 days. The results showed that at control salinity level, there was no significant difference between putrescine treatments on Henna seed germination indices except the seed vigor index. The interaction between putrescine and salinity stress showed that pre-treatment with putrescine alleviated the negative effects of 50 and 100 mM salinity stress on the germination rate. However, it increased the negative effects of 150 mM salinity stress on germination percentage, radicle length, plumule length, seedling fresh weight, seed vigor index and α-amylase activity in comparison to control. The results showed that germination of Henna seeds is sensitive to salinity stress and they are only able to tolerate low levels of salinity (50 mM). According to a significant correlation between α-amylase activity with germination percentage (r= 0/98), it can be concluded that probably the change in α-amylase activity decreased the germination percentage. | ||
| کلیدواژه‌ها [English] | ||
| Germination percentage, Polyamine, Salinity tolerance, Seed vigor index | ||
| مراجع | ||
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Abdul‐Baki, A. A., & Anderson, J. D. (1973). Vigor determination in soybean seed by multiple criteria 1. Crop Science, 13(6), 630-633. https://doi.org/10.2135/cropsci1973.0011183X001300060013x Adda, A., Regagba, Z., Latigui, A., & Merah, O. (2014). Effect of salt stress on α-amylase activity, sugars mobilization & osmotic potential of Phaseolus vulgaris L. seeds var. 'Cocorose' and 'Djadida' during germination. Journal of Biological Sciences, 14(5), 370-375. https://doi.org/10.3923/jbs.2014.370.375 Alcázar, R., Bueno, M., & Tiburcio, A. F. (2020). Polyamines: Small amines with large effects on plant abiotic stress tolerance. Cells, 9(11), 2373. https://doi.org/10.3390/cells9112373 Ali, R. M. (2000). Role of putrescine in salt tolerance of Atropa belladonna plant. Plant Science, 152(2), 173-179. https://doi.org/10.1016/S0168-9452(99)00227-7 Ali, R. M., Abbas, H. M., & Kamal, R. K. (2009). The effects of treatment with polyamines on dry matter and some metabolites in salinity–stressed chamomile and sweet majoram seedlings. Plant, Soil and Environment, 55(11), 477-483. Ambika, S., Sujatha, K., & Balakrishnan, K. (2019). Seed priming treatments on seedling quality of henna (Lawsonia inermis L.) seeds. Acta Horticulture, 1241, 375-380. https://doi.org/10.17660/ActaHortic.2019.1241.54 Bahrasemani, S., Seyedi, A., Fathi, S., & Jowkar, M. (2023). The seed priming using putrescine improves germination indices and seedlings morphobiochemical responses of indigo (Indigofera tinctoria) under salinity stress. Journal of Medicinal Plants and By-Products, 13(1), 179-188. https://doi.org/10.22034/jmpb.2023.128870 Borromeo, I., Domenici, F., Del Gallo, M., & Forni, C. (2023). Role of polyamines in the response to salt stress of tomato. Plants, 12(9), 1855. https://doi.org/10.3390/plants12091855 Chaudhary, G., Goyal, S., & Poonia, P. (2010). Lawsonia inermis Linnaeus: A phytopharmacological review. Int J Pharm Sci Drug Res, 2(2), 91-98. Chen, D., Shao, Q., Yin, L., Younis, A., & Zheng, B. (2019). Polyamine function in plants: Metabolism, regulation on development, and roles in abiotic stress responses. Frontiers in Plant Science, 9, 1945. https://doi.org/10.3389/fpls.2018.01945 Dalziell, E. L., Lewandrowski, W., & Merritt, D. J. (2020). Increased salinity reduces seed germination and impacts upon seedling development in Nymphaea L. (Nymphaeaceae) from northern Australia’s freshwater wetlands. Aquatic Botany, 165, 103235. https://doi.org/10.1016/j.aquabot.2020.103235 Enneb, H., & Mohammad Ayaz, A. (2016). Germination behaviour of Lawsonia inermis L. as influenced by polyethylene glycol (PEG). Dialogo, 3(1), 173-179. Enneb, H., Belkadhi, A., & Ferchichi, A. (2016). Physiological adaptations of henna plant (Lawsonia inermis L.) to different irrigation conditions in Tunisian arid region. JAPS: Journal of Animal & Plant Sciences, 26(4), 1026-1033. URL: http://www.thejaps.org.pk/docs/v-26-04/18.pdf Farahbakhsh, H., Pasandi Pour, A., & Reiahi, N. (2017). Physiological response of henna (Lawsonia inermis L.) to salicylic acid and salinity. Plant Production Science, 20(2), 237-247. https://doi.org/10.1080/1343943X.2017.1299581 Farsaraei, S., Mehdizadeh, L., & Moghaddam, M. (2021). Seed priming with putrescine alleviated salinity stress during germination and seedling growth of medicinal pumpkin. Journal of Soil Science and Plant Nutrition, 21(3), 1782-1792. https://doi.org/10.1007/s42729-021-00479-z Fernández-García, N., Olmos, E., Bardisi, E., García-De la Garma, J., López-Berenguer, C., & Rubio-Asensio, J. S. (2014). Intrinsic water use efficiency controls the adaptation to high salinity in a semi-arid adapted plant, henna (Lawsonia inermis L.). Journal of Plant Physiology, 171(5), 64-75. https://doi.org/10.1016/j.jplph.2013.11.004 Hamidi Moghaddam, A. (2022). Effect of mechanical and chemical treatments on germination characteristic, total phenolic compound and enzyme activity of henna seeds (Lawsonia inermis L.). Iranian Journal of Seed Science and Research, 8(4), 396-410. https://doi.org/10.22124/jms.2021.5288 Hammami, H., Saadatian, B., & Hosseini, S. A. H. (2020). Geographical variation in seed germination and biochemical response of milk thistle (Silybum marianum) ecotypes exposed to osmotic and salinity stresses. Industrial Crops and Products, 152, 112507. https://doi.org/10.1016/j.indcrop.2020.112507 Hartman, H., D. Kester, & Davis, F. (1990). Plant propagation: Principles and practices. Prentice Hall International Editions. Ibrahim, E. A. (2016). Seed priming to alleviate salinity stress in germinating seeds. Journal of Plant Physiology, 192, 38-46. https://doi.org/10.1016/j.jplph.2015.12.011 ISTA. (1979). The germination test. International Seed Testing Association. Seed Science and Technology, 4, 23-28. Khan, H. A., Ziaf, K., Amjad, M., & Iqbal, Q. (2012). Exogenous application of polyamines improves germination and early seedling growth of hot pepper. Chilean Journal of Agricultural Research, 72(3), 429-433. Krishnamurthy, L., Ito, O., Johansen, C., & Saxena, N. P. (1998). Length to weight ratio of chickpea roots under progressively receding soil moisture conditions in a Vertisol. Field Crops Research, 58(3), 177-185. https://doi.org/10.1016/S0378-4290(98)00093-8 Lal, G., Roy, P. K., & Singh, Y. V. (2007). Effect of different treatments on germination behaviour of henna (Lawsonia inermis L.) seeds. SAARC Journal of Agriculture, 5(2), 67-74. http://www.saarcagri.net Li, Z., Peng, Y., Zhang, X. Q., Ma, X., Huang, L. K., & Yan, Y. H. (2014). Exogenous spermidine improves seed germination of white clover under water stress via involvement in starch metabolism, antioxidant defenses and relevant gene expression. Molecules, 19(11), 18003-18024. https://doi.org/10.3390/molecules191118003 Liu, L., Xia, W., Li, H., Zeng, H., Wei, B., Han, S., & Yin, C. (2018). Salinity inhibits rice seed germination by reducing α-amylase activity via decreased bioactive gibberellin content. Frontiers in Plant Science, 9, 275. https://doi.org/10.3389/fpls.2018.00275 Marzougui, N., Sabbahi, S., Guasmi, F., Hammami, A., Haddad, M., & Rejeb, S. (2018). Effects of wastewater quality on Henna (Lawsonia inermis L.) germination and seedling growth: A case study, Tunisia. International Journal of Environment, Agriculture and Biotechnology, 3(1), 147-157. https://doi.org/10.22161/ijeab/3.1.19 Mohamadi, F., Bagheri, N., Kiani, G., & Babaeian Jelodar, N. (2018). Evaluation of reaction of some rice genotypes to salinity stress at germination stage. Journal of Crop Breeding, 10(27), 20-30. https://doi.org/10.29252/jcb.10.27.20 Mustafavi, S. H., Naghdi Badi, H., Sękara, A., Mehrafarin, A., Janda, T., Ghorbanpour, M., & Rafiee, H. (2018). Polyamines and their possible mechanisms involved in plant physiological processes and elicitation of secondary metabolites. Acta Physiologiae Plantarum, 40, 1-19. https://doi.org/10.1007/s11738-018-2671-2 Najar, B., Pistelli, L., Marchioni, I., Pistelli, L., Muscatello, B., De Leo, M., & Scartazza, A. (2020). Salinity-induced changes of photosynthetic performance, lawsone, VOCs, and antioxidant metabolism in Lawsonia inermis L. Plants, 9(12), 1797. https://doi.org/10.3390/plants9121797 Noroozisharaf, A., Kaviai, M., & Rasouli, M. (2021). Effect of brassinosteroid on morphological and physiological traits of garden thyme (Thymus vulgaris) in salinity stress. Iranian Journal of Seed Sciences and Research, 8(1), 63-76. https://doi.org/10.22124/jms.2021.5203 Orhan, E., Uzundumlu, F., Yiğider, E., & Aydin, M. (2020). The effect of putrescine on DNA methylation on cabbage plant under salt stress conditions. Turkish Journal of Agriculture and Forestry, 44(3), 301-311. https://doi.org/10.3906/tar-1911-38 Parida, A. K., Das, A. B., Mittra, B., & Mohanty, P. (2004). Salt-stress induced alterations in protein profile and protease activity in the mangrove Bruguiera parviflora. Zeitschrift für Naturforschung C, 59(5-6), 408-414. https://doi.org/10.1515/znc-2004-5-622 Parihar, S. S., Dadlani, M., Mukhopadhyay, D., & Lal, S. K. (2016). Seed dormancy, germination and seed storage in henna (Lawsonia inermis). The Indian Journal of Agricultural Sciences, 86(9), 1201-1207. https://doi.org/10.56093/ijas.v86i9.61520 Reis, R. S., de Moura Vale, E., Heringer, A. S., Santa-Catarina, C., & Silveira, V. (2016). Putrescine induces somatic embryo development and proteomic changes in embryogenic callus of sugarcane. Journal of Proteomics, 130, 170-179. https://doi.org/10.1016/j.jprot.2015.09.029 Sarhadi, H., Daneshian, J., Valadabadi, S. A., & Heidari Sharafabad, H. (2014). Study of irrigation deficit and N fertilizer effect on reproductive components of henna ecotypes in Jiroft, Iran. Biological Forum: An International Journal, 8(1), 80-87. Serraj, R., & Sinclair, T. R. (2002). Osmolyte accumulation: Can it really help increase crop yield under drought conditions? Plant, Cell & Environment, 25(2), 333-341. https://doi.org/10.1046/j.1365-3040.2002.00754.x Shuba, A. C., Channnakeshava, B. C., Bhanuprakash, K., & Kumar, A. (2018). Study on seed quality performance and enzymatic activity after dormancy breaking in henna. Journal of Pharmacognosy and Phytochemistry, 7(1), 105-108. Singh, D. K., Luqman, S., & Mathur, A. K. (2015). Lawsonia inermis L.–A commercially important primaeval dying and medicinal plant with diverse pharmacological activity: A review. Industrial Crops and Products, 65, 269-286. https://doi.org/10.1016/j.indcrop.2014.11.025 Srivastava, A. K., Lokhande, V. H., Patade, V. Y., Suprasanna, P., Sjahril, R., & D’Souza, S. F. (2010). Comparative evaluation of hydro-, chemo-, and hormonal-priming methods for imparting salt and PEG stress tolerance in Indian mustard (Brassica juncea L.). Acta Physiologiae Plantarum, 32, 1135-1144. https://doi.org/10.1007/s11738-010-0505-y Suchak, H., & Pandya, R. V. (2020). Effect of spermine and putrescine on germination and growth of Vigna radiata (L.) R. Wilczek seeds. In Proceedings of the National Conference on Innovations in Biological Sciences (NCIBS). http://dx.doi.org/10.2139/ssrn.3585124 Varghese, K. J., Silvipriya, K. S., Resmi, S., & Jolly, C. I. (2010). Lawsonia inermis (henna): A natural dye of various therapeutic uses—a review. Inventi Impact: Cosmeceuticals. Varner, J. E. (1964). Gibberellic acid controlled synthesis of α-amylase in barley endosperm. Plant Physiology, 39(3), 413–415. https://doi.org/10.1104/pp.39.3.413 Yang, L. I. U., Hong, X. U., WEN, X. X., & LIAO, Y. C. (2016). Effect of polyamine on seed germination of wheat under drought stress is related to changes in hormones and carbohydrates. Journal of Integrative Agriculture, 15(12), 2759-2774. https://doi.org/10.1016/S2095-3119(16)61366-7 | ||
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