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تاثیر همزمان نانوسید، قارچکش و قارچ تریکودرما بر گیاهچههای کلزا مبتلا به پوسیدگی سفید ساقه ناشی از Sclerotinia sclerotiorum | ||
| مهار زیستی در گیاهپزشکی | ||
| دوره 10، شماره 2، اسفند 1401، صفحه 109-124 اصل مقاله (654.31 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2023.363789.350 | ||
| نویسندگان | ||
| فاطمه سلطانی نژاد* 1؛ کامران رهنما2؛ سید جواد صانعی3 | ||
| 1دانشجوی دکتری، گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران. | ||
| 2دانشیار،گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران. | ||
| 3استادیار، گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران. | ||
| چکیده | ||
| در این بررسی، اثر بازدارندگی گونه های تریکودرما به همراه نانوسید و قارچ کش برای مبارزه تلفیقی با قارچ بیمارگر Sclerotinia sclerotiorum مورد مطالعه قرار گرفته است. بدین منظور، اثر جداگانه سه جدایه Trichoderma harzianum، یک جدایهT. virens، یک جدایه T. atroviride و یک جدایهT. koningii بر سه جدایه عامل بیمارگر (SS1، SS2 و SS3) در محیط کشت PDA دربردارنده 5 سطح از نانوسید(0، 30، 60، 100 و 130 پی پی ام)، به روش کشت متقابل در آزمایشگاه بررسی شد. سپس اثر T. harzianum (1211)، T. koningii (iso2) و T. virens (6011) به همراه نانوسید (سطوح 10 و 30 پی پی ام) و قارچ کش ایپرودیون–کاربندازیم (سطوح 2 و 5 پیپیام) بر یک جدایه عامل بیمارگر درشرایط گلخانه در قالب طرح کاملاً تصادفی با چهار تکرار، مورد ارزیابی قرار گرفت. در کشت متقابل، کمترین درصد بازدارندگی از رشد قارچ اسکلروتینیا، 6 درصد بود که متعلق به تیمارS. sclerotiorum (SS2) × Trichoderma virens (6011) در سطح 10 پی پی ام نانوسید بود. در گلخانه، تریکودرما به دو روش پوشش سطحی بذر و نیز مخلوط با خاک، بکار برده شد و صفات مورد ارزیابی شامل ارتفاع بوته و وزن تر و خشک گیاه بود. در آزمایش اول به روش پوشش سطحی بذر با سوسپانسیون گونه های تریکودرما، بیشترین میزان وزن تر گیاه به تیمارT. harzianum (1211) و بیشترین ارتفاع نیز به تیمار T. koningii (iso2) مربوط بود. در آزمایش دوم به روش کاربرد گونه های تریکودرما به صورت مخلوط با خاک، بیشترین وزن تر و خشک گیاه در تیمار T. harzianum (1211) + 30 پی پی ام نانوسید و بیشترین ارتفاع در تیمار T. harzianum (1211) + 2 پی پی ام قارچ(P≤0.01) مشاهده شد. در مجموع، تیمارهایی که گونه های تریکودرما به تنهایی به کار رفته بود با تیمارهایی که نانوسید و قارچ کش هم اضافه شده بود، تفاوت معنی داری را در وزن تر، خشک و ارتفاع گیاه نشان نداد. به علاوه کاربرد گونه های تریکودرما به تنهایی نسبت به تلفیق آن با نانوسید و قارچ کش، تاثیر بهتری بر صفات رویشی گیاهچه های کلزای مبتلا به این بیماری داشت. | ||
| کلیدواژهها | ||
| قارچکش ایپرودیون-کاربندازیم؛ گونههای تریکودرما؛ مدیریت تلفیقی؛ نانوسید؛ Sclerotinia sclerotiorum | ||
| عنوان مقاله [English] | ||
| Simultaneous effects of Nanocide, Fungicide and Trichoderma fungi on the Canola Seedlings Affected by Stem Rot Pathogen, Sclerotinia sclerotiorum | ||
| نویسندگان [English] | ||
| fatemeh soltani nezhad1؛ kamran rahnama2؛ seyed javad sanei3 | ||
| 11.,Ph.D. Student,, Department of Plant Protection Faculty of Plant production, Agricultural Sciences and Natural Resources of Gorgan University, Iran. | ||
| 2Associated Professor, Department of Plant Protection Faculty of Plant production, Agricultural Sciences and Natural Resources of Gorgan University, Iran. | ||
| 3Assistant Professor, Department of Plant Protection Faculty of Plant production, Agricultural Sciences and Natural Resources of Gorgan University, Iran. | ||
| چکیده [English] | ||
| In this research, the compatibility of Trichoderma species along with Nanocide and fungicide for the integrated management of this pathogenic fungi Sclerotinia sclerotiorum, has been studied. For this purpose, the effect of three isolates of Trichoderma harzianum, one isolate of T. virens, one isolate of T. atroviride and one isolate of T. koningii on the three isolates of pathogenic agent (SS1, SS2, SS3) in PDA medium containing 5 levels of Nanocide (0,30,60,100,130) by dual culture technique in the laboratory condition were studied. Then the effects of T. harzianum (1211), T koningii (iso2) and T. virens (6011) with two levels of Noniocid (10 & 30 ppm) and Iprodione+carbendazim fungicide (2 & 5 ppm) on one isolate of pathogenic agent in greenhouse condition, were evaluated. The lowest inhibition of mycelia growth of S. sclerotiorum in PDA medium with Nanocide, was 6%, that indicated for treatment S. sclerotiorum (SS2) × T. virens (6011) at level 10 ppm. In greenhouse condition, Trichoderma spp were tested in two stages, seed coated with Trichoderma conidia cell suspension and use Trichoderma mixed with soil. The evaluated parameters were height and wet and dry weight of the plant. The first experiment, was seed coated with Trichoderma conidia cell suspension, the most fresh weight was for T. harzianum (1211) as control and the most height was for T. koningii (iso2) alone. The second experiment, was for Trichoderma mixed with soil showed a significant increase in the most fresh and dry weight were for T. harzianum (1211) + 30 ppm Nanocide treatment and the most height of seedlings was also for T. harzianum (1211) + 2 ppm Iprodione–carbendazim (P≤0.01(. the statistical grouping showed that the treatments in which Trichoderma species were used alone showed no significant difference with treatments with Nanocid and fungicide in the wet and dry weight and height plant. Beside, using Trichoderma alone rather than the combination of Nanocid and fungicide, showed the better effect on growth parameters of the infected canola. | ||
| کلیدواژهها [English] | ||
| Integrated management, Sclerotinia sclerotiorum, Trichoderma, Nanocid, Iprodione+carbendazim fungicide | ||
| مراجع | ||
|
Alamdarlou, M.R., Zaman Mirabadi, A., Esmailifar, A. & Foroozan, K. 2009. Study on the effect of number of sprayings with fungicides on rapeseed Sclerotinia stem rot control. The 17th Australian plant pathology society conference 225–226, Newcastle, Australia. Alamdarlou, M.R., Salari, M., Aghajani, M.A., Panjekeh, N. & Sabbagh, S.K. 2018. Effect of Some Fungicides on Causal Agent of Sclerotinia Stem Rot Disease of Rapeseed in Mazandaran Province. Journal of Applied Research in plant protection, 7(3): 103–115. (In Persian with English Summary). Alkooranee, J.T., Aledan, T.R., Ali, A.K., Lu, G., Zhang, X., Wu, J., Fu, C. & Li, M. 2017. Detecting the hormonal pathways in oilseed rape behind induced systemic resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum, 12(1). Ashrafi, S.J., Falahati Rastegar, M., Jafarpour, B., Tahmasebi, N. & Anil Kumar, S. 2010. Study on the effectiveness of silver Nano particles in controlling Fusarium wilting of lentil. 19th Iranian plant protection congress 7–8, Tehran, Iran. Chaparro, A.P., Carvajal, L.H. & Orduz, S. 2011. Fungicide tolerance of Trichoderma asperelloides and T. harzianum strains. Agricultural Sciences, 2(3): 301–307. Cho, K.H., Park, J.E., Osaka, T. & Park, S.G. 2005. The study of antimicrobial activity and preservative effects of Nanosilver ingredient. Electrochimica Acta, 51(5): 956– 960. Clarkson, J.P., Phelps, K., Whipps, J.M., Young, C.S., Smith, J.A. & Watling, M. 2004. Forecasting Sclerotinia disease on lettuce: Toward developing a prediction model for carpogenic germination of Sclerotinia. Phytopathology, 94(3): 268–279. Clement, J.L. & Jarret, P.S. 1994. Antimicrobial silver. Metal–Based Drugs, 1(5–6): 467–482. Dalili, A., Bakhtiari, S., Barari, H. & Aldaghi, M. 2015. Effect of some fungicides against the growth inhibition of Sclerotinia sclerotiorum mycelial compatibility groups. Journal of Plant Protection Research, 55(4): 354–361. (In Persian with English Summary). Dehghan, M.A. 2010. Investigation and comparison effect of Nanosilver and Defenconazole fungicide in control seed treatment of wheat covered smut. Proceedings 19th Iranian plant protection congress. Tehran, Iran, 836–837. Derbyshire, M.C. & Denton Giles, M. 2016. The control of sclerotinia stem rot on oilseed rape (Brassica napus): Current practices and future opportunities. Plant Pathology, 65: 859–877. Elad, Y., Katan, J. & Chet, I. 1980. Physical, biological and chemical control integrated for soilborne disease in potato. Phytopathology, 70(5): 418–442. Expert, J.M. & Digat, B. 1995. Biocontrol of Sclerotinia wilt of sunflower by Pseudomonas putida strains. Canadian Journal of Microbiology, 41(8): 685–691. Fernando, W.G.D., Nekkeeran, S. & Zhang, Y. 2004. Ecofriendly methods in combating Sclerotinia sclerotiorum(lib.) de Bary. Developmental and Environmental Biology, (1): 329–347. Gajbhiye, M., Kesharwani, J., Ingle, A., Gade, A. & Rai, M. 2009. Fungus–mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomedicine, 5(4): 382–386. Glick, BR. 2020. Biocontrol of Bacteria and Fungi, Beneficial Plant–Bacterial Interactions. Springer, 181–230. Habibi, R., Rahnama, K. & Taghinasab, M. 2015. Evaluating the effectiveness of native Trichoderma species in fproduction of extracellular enzymes during interaction with plant pathogenic fungus Fusarium oxysporum. Applied Research in Plant Protection, 4(2): 73–85. (In Persian with English Summary). Harman, G.E. 1996. Trichoderma for biocontrol of plant pathogens: from basic research to commercialized products. Proceedings of Cornell community conference on biological control April 11–13. http://www.nysaes.cornell.edu/ent/bcconf/talks /harman.html (verified August 12, 2011) 52. Harman, G.E., Taylor, A.G Harman, G.E., Howell, C.R., Vibterbo, A., Chet, I. & Lorito, M. 2004. Trichoderma species opportunistic, avirulent plant symbionts. Microbiology, 2(1): 43–56. Hornby, D. 1997. Biological control of Soil–borne Plant Pathogens. CAB International Publishing Division, 826 pp. Howell, C.R., Devay, J.E., Garber, R.H. & Batson, W.E. 1997. Field control of cotton seedling diseases with Trichoderma virens in combination with fungicide seed treatments. Cotton Science, 1(1): 15–20. Jabbari, H., Mansouri, N., Abdollahi, A., Chehrehei, M. & Naddafi K. 2009. Studying the effect of nanosilver painting on control of air–transmitted fungi. Health and environment, 2(1): 28–35. (In Persian with English Summary). Jambhulkar, P.P., Sharma, P., Manokaran, R., Lakshman, D.K., Rokadia, P. & Jambhulkar, N. 2018. Assessing synergism of combined applications of Trichoderma harzianum and Pseudomonas fluorescens to control blast and bacterial leaf blight of rice. European Journal of Plant Pathology, 152: 747–757 Lehner, M.S., De Paula Junior, T.J., Del Ponte, E.M., Mizubuti, E.S.G. & Pethybridge, S.J. 2017. Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure. PLoS ONE, 12(3). e0173915. Li, T., Huang, W. & Yu, H. 2022. Synergetic Antimicrobial Effect of Silver Nanoparticles Conjugated with Iprodione against Valsa mali. Materials, 15(15): 5147. Kia, S. & Rahnama, K. 2016. Study on the efficiency of Trichoderma isolates in controlling charcoal rot disease of soybean caused by Macrophomina phaseolina under greenhouse conditions. Biocontrol in Plant Protection 4(1): 1–10. (In Persian with English Summary). Lederer, W., Lorenz, K.H. & Seemuller, E. 1992. Studies on Antagonistic effects of Trichoderma isolates against Phytophthora cactorum. Phytopathology, 136(2): 154–164. Lipovsky, A., Nitzan, Y., Gendanken, A. & Lubart, R. 2011. Antifungal activity of ZNO nanoparticles–the role of ROS mediated cell injury. Nanotechnology, 22(10): 101–105. Liu, C.Q., Du, D.Z. & Zou, C.S. 1990. Initial studies on tolerance to Sclerotinia sclerotiorum (Lib De Bary.) in Brassica napus L. Proceedings of Symposium of China International Rapeseed Sciences. Shanghai, China, 70–71. Mahdizadeh, V., Safaie, N. & Khelghatibana, F. 2015. Evaluation of antifungal activity of silver nanoparticles against some phytopathogenic fungi and Trichoderma harzianum. Journal of cop protection, 4(3): 291–300. Montazernia, B. 2008. Evaluation of integrated management potential to control of charcoal rot (Macrophomina phaseolina) in soybean by Trichoderma spp. M.sc. Thesis, Gorgan university of Agricultural sciences and Natural Resources. Mukherjee, P.K., Horwitz, B.A., Singh, U.S., Mukherjee, M. & Schmoll, M. 2013. Trichoderma: biology and applications, CABI 230–246. Naseripour, T., Nasrollah Nezhad, S., Shahbazi, S. & Rahnama, K. 2015. Using gamma–ray to increased exoglucanase activity in Trichoderma and improvement of Sclerotinia rot of canola biocontrol. Biological Forum, 7(2): 57–60. Rahnama, K., Vakili, Z. & Razavi, S.I. 2004. Distribution of canola stems white rot (Sclerotinia sclerotiorum) in Golestan province and its control. The Second Proceeding of National oil seeds festival. Gorgan, Iran, 34–35. Safarimotlagh, M.R. & Abolghasemi, S. 2019. Biological control of crown rot disease of canola by isolates of Trichoderma in vitro and under greenhouse conditions. Plant protection, 42(2): 1–18. Sharma, P., Meena, P.D., Verma, P.R., Saharan, G.S., Mehta, N., Singh, D. & Kumar, A. 2015. Sclerotinia sclerotiorum (Lib.) de Bary causing Sclerotinia rot in oilseed Brassicas: A. review. Journal of oilseed Brassicae 6: 1–44. Sharma, J., Singh, V.K., Kumar, A., Shankarayan, R. & Mallubhotla, S. 2018. Role of Silver Nanoparticles in treatment of plant disease. Microbial Biotechnology, 2: 435–454. Tian, B., Xie, J., Fu, Y., Cheng, J., Li, B.O., Chen, T., Zhao, Y., Gao, Z., Yang, P. & Barbetti, M.J. 2020. A cosmopolitan fungal pathogen of dicots adopts an endophytic lifestyle on cereal crops and protects them from major fungal diseases. The ISME Journal, 14: 3120–3135. Vakili Zarj, Z., Rahnama, K. & Rahnama, M. 2013. The comparative growth and determination of the isolates reaction of Sclerotinia sclerotiorum on oilseed rape cultivars. International journal of agronomy and plant production, 4(5): 928–935. | ||
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