اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها3,006
تعداد مقالات33,642
تعداد مشاهده مقاله44,579,016
تعداد دریافت فایل اصل مقاله31,683,627
Nadaf, Rubeen, Nadaf, Parveen, Toragall, Makhadumsab, C T, Shivasharana. (1403). Response surface methodology for optimization of media components for production of lipase from Bacillus Subtilis KUBT4. سامانه مدیریت نشریات علمی, 79(3), 659-668. doi: 10.32592/ARI.2024.79.3.659
Rubeen Dadakalandar Nadaf; Parveen Nadaf; Makhadumsab Toragall; Shivasharana C T. "Response surface methodology for optimization of media components for production of lipase from Bacillus Subtilis KUBT4". سامانه مدیریت نشریات علمی, 79, 3, 1403, 659-668. doi: 10.32592/ARI.2024.79.3.659
Nadaf, Rubeen, Nadaf, Parveen, Toragall, Makhadumsab, C T, Shivasharana. (1403). 'Response surface methodology for optimization of media components for production of lipase from Bacillus Subtilis KUBT4', سامانه مدیریت نشریات علمی, 79(3), pp. 659-668. doi: 10.32592/ARI.2024.79.3.659
Nadaf, Rubeen, Nadaf, Parveen, Toragall, Makhadumsab, C T, Shivasharana. Response surface methodology for optimization of media components for production of lipase from Bacillus Subtilis KUBT4. سامانه مدیریت نشریات علمی, 1403; 79(3): 659-668. doi: 10.32592/ARI.2024.79.3.659

Response surface methodology for optimization of media components for production of lipase from Bacillus Subtilis KUBT4

Archives of Razi Institute
مقاله 28، دوره 79، شماره 3، مرداد و شهریور 2024، صفحه 659-668    اصل مقاله (961.1 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32592/ARI.2024.79.3.659
نویسندگان
Rubeen Dadakalandar Nadaf1؛ Parveen Nadaf2؛ Makhadumsab Toragall3؛ Shivasharana C T* 4
1KAHER’s Dr. Prabhakar Kore Basic Science Research Centre, Belagavi-590010, Karnataka, India.
2Shahin Independent PU College, Old P.B Road, Nipani-591237. Karnataka, India.
3Department of Applied Genetics, Karnatak University, Dharwad, Karnataka, India.
4Department of Biotechnology and Microbiology, Karnatak University, Dharwad-580003, Karnataka, India.
چکیده
Lipases belong to a class of triacylglycerol hydrolases possessing a lot of potential applications due to their various physical properties. Most of the lipase producers are extracellular in nature are produced using solid-state fermentation and submerged fermentation methods. The fungal, mycelial, and the yeast lipases are produced using various solid substrates through solid-state fermentation method. This method is cost effective and hence widely used in industries to produce lipase using fungi. Whereas lipases from bacteria, were produced using submerged fermentation. The optimization of media is a main requirement for increasing the quantitative yield by overproduction of enzymes. The optimization of media is a main requirement for increasing the quantitative yield by overproduction of enzymes. Different parameters such as pH, temperature, agitation speed, inoculum size, incubation time, carbon and nitrogen sources have been of great importance for the researchers to design economical media. The OFAT is a one-dimensional approach that is laborious and time-consuming and does not consider interactions between the factors. The limitations of OFAT method can be reduced by employing techniques such as Plackett-Burman design (PBD) and response surface methodology (RSM). The PBD is a method to screen the variables that influence the production and remove the non-significant factors to attain a lesser and manageable set of factors. Subsequently, the chosen significant factors are optimized by RSM that assists to study the interactions of different factors. The RSM comprises of central composite design (CCD) to fit a second-order polynomial equation. In this study, the effect of temperature, tryptone, inoculum size and incubation time on the lipase production were analysed by Plackett-Burman design screening experiments. The experiments were designed using four variable Central composite design of RSM using Design Expert software. This model predicted optimal activity of lipase of 58.53 U/mL at 1.5% tryptone, 10 mL inoculum size, incubated for 48 hrs at 34℃. This experiment was further validated and optimal activity of lipase of 57.85 U/mL was observed. Thus, RSM model enhanced the production of lipase and can be applied for the maximum yield of lipase.
کلیدواژه‌ها
Bacillus subtilis KUBT4؛ Lipase؛ Plackett-Burman design؛ Response Surface Methodology
سایر فایل های مرتبط با مقاله
مراجع
  1. Treichel H, De Oliveira D, Mazutti MA, Di Luccio M, Oliveira JV. A review on microbial lipases production. Food Bioproc Tech. 2010;3:182-96.
  2. Colla LM, Primaz AL, Benedetti S, Loss RA, Lima MD, Reinehr CO, Bertolin TE, Costa JA. Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi. Braz J Microbiol. 2016;47:461-7.
  3. Nadaf RD, Thimmappa DS. Optimization of the media components by one factor at a time methodology to enhance lipase production by Bacillus Substilis KUBT4. Int J Sci Technol Res. 2020;9(01).
  4. Palaniyappan M, Vijayagopal V, Viswanathan R, Viruthagiri T. Screening of natural substrates and optimization of operating variables on the production of pectinase by submerged fermentation using Aspergillus niger MTCC 281. Afr J Biotechnol. 2009;8(4).
  5. Montgomery DC, Keats JB, Perry LA, Thompson JR, Messina WS. Using statistically designed experiments for process development and improvement: an application in electronics manufacturing. Robot Comput Integr Manuf. 2000;16(1):55-63.
  6. Ghaffari-Moghaddam M, Yekke-Ghasemi Z, Khajeh M, Rakhshanipour M, Yasin Y. Application of response surface methodology in enzymatic synthesis: A review. Russ J Bioorg Chem. 2014;40:252-62.
  7. Joshi R, Kuila A. Lipase and their different industrial applications: A review. Braz J Bio Sci. 2018;5(10):237-47.
  8. Coradi GV, da Visitação VL, de Lima EA, Saito LY, Palmieri DA, Takita MA, de Oliva Neto P, de Lima VM. Comparing submerged and solid-state fermentation of agro-industrial residues for the production and characterization of lipase by Trichoderma harzianum. Ann Microbio. 2013;63:533-40.
  9. Abdel-Fattah YR. Optimization of thermostable lipase production from a thermophilic Geobacillus using Box-Behnken experimental design. Biotechnol Lett. 2002;24(14):1217-22.
  10. Kalil SJ, Maugeri F, Rodrigues MI. Response surface analysis and simulation as a tool for bioprocess design and optimization. Process Biochem. 2000;35(6):539-50.
  11. Haaland PD. Experimental design in biotechnology. 1st ed. CRC Press Inc; Boca Raton: Florida; 1989.
  12. Kiran KR, Manohar B, Karanth NG, Divakar S. Response surface methodological study of esterification of lactic acid with palmitic acid catalyzed by immobilized lipases from Mucor miehei and porcine pancreas. Eur Food Res Technol. 2000;211:130-5.
  13. Bidin H, Basri M, Radzi SM, Ariff A, Rahman RN, Salleh AB. Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM). Ind Crops Prod. 2009;30(2):206-11.
  14. Osorio NM, Ferreira-Dias S, Gusmão JH, Da Fonseca MM. Response surface modelling of the production of ω-3 polyunsaturated fatty acids-enriched fats by a commercial immobilized lipase. J Mol Catal B Enzym. 2001;11(4-6):677-86.
  15. Nadaf, R. D., & Shivasharana, C. T. Extracellular lipase from bacillus subtilis kubt4: isolation, identification and characterization. International Journal of Pharmaceutical Sciences and Research. 2019 May 1;10(5):2545-2549.
  16. Soo EL, Salleh AB, Basri M, Rahman RA, Kamaruddin K. Response surface methodological study on lipase-catalyzed synthesis of amino acid surfactants. Process Biochem. 2004;39(11):1511-8.
  17. Yadav GD, Lathi PS. Kinetics and mechanism of synthesis of butyl isobutyrate over immobilised lipases. Biochem Eng J. 2003;16(3):245-52.
  18. Shieh CJ, Liao HF, Lee CC. Optimization of lipase-catalyzed biodiesel by response surface methodology. Bioresour Techno. 2003;88(2):103-6.
  19. Jahantigh M. Characteristics of some digestive enzymes in sobaity, Sparidentex hasta. Iran J Vet Med. 2015;9(3).
  20. Ali Anvar SA, Nowruzi B, Afshari G. A Review of the Application of Nanoparticles Biosynthesized by Microalgae and Cyanobacteria in Medical and Veterinary Sciences. Iran J Vet Med. 2023;17(1).
  21. Peidaei F, Ahari H, Anvar A, Ataei M. Nanotechnology in Food Packaging and Storage: A Review. Iran J Vet Med. 2021;15(2).
آمار
تعداد مشاهده مقاله: 11,916
تعداد دریافت فایل اصل مقاله: 366


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب